oviposition attractancy of bacterial culture filtrates: response of culex quinquefasciatus.oviposition attractants could be used for monitoring as well as controlling mosquitoes by attracting them to lay eggs at chosen sites. in the present study, culture filtrates of seven bacterial species were tested for their attractancy against gravid females of culex quinquefasciatus. when their oviposition active indices (oai) were studied, the culture filtrates of bacillus cereus and pseudomonas fluorescens exhibited oviposition attractancy (oai = > 0.3) at 100 ppm and the oai were respectivel ...200212048566
isolation of a pseudomonas fluorescens metabolite/exotoxin active against both larvae and pupae of vector mosquitoes.a formulation was developed from the metabolite(s) of a novel pseudomonas fluorescens migula strain (vcrc b426) and tested against 4th-instar larvae and pupae of three species of vector mosquitoes, anopheles stephensi liston, culex quinquefasciatus say and aedes aegypti (l). the larvae and pupae of an. stephensi were the most susceptible to the formulation, followed by those of c. quinquefasciatus and ae. aegypti, in that order, and the dosage requirement for pupal mortality was less than that r ...200312558096
di-rhamnolipid is a mosquito pupicidal metabolite from pseudomonas fluorescens (vcrc b426).pseudomonas fluorescens migula (vcrc b426) produces a secondary metabolite, which was found to be active against pupae of vector mosquitoes namely culex quinquefasciatus, anopheles stephensi and aedes aegypti. the mosquito pupicidal metabolite from p. fluoescens was mass produced and separated by ethyl acetate extraction and purified further by silica gel column chromatography, fplc, hplc and tlc. the purified metabolite was characterized by nmr, ft-ir, lc-ms and maldi-tof. the ft-ir, (1)h and ( ...201525912083
binding sites of mosquitocidal toxins of pseudomonas fluorescens and bacillus subtilis on pupae and larvae of culex quinquefasciatus.two of the potential bacterial isolates, viz., pseudomonas fluorescens (vcrc b-426) and bacillus subtilis (vcrc b-471) whose toxins kill the mosquito pupae/larvae have been identified at our center. as the mode of action of these bacteria are not known, an attempt was made to find out the binding sites of the toxic proteins through immunological methods. antibodies were raised in balb/c mice and egg yolk system of chicken layers against the mosquitocidal proteins. the antibodies showed specific ...201524624898
field evaluation of a formulation of pseudomonas fluorescens against culex quinquefasciatus larvae and pupae.vcrc b426, 0.09% emulsifiable concentrate (ec) formulation developed from a metabolite of pseudomonas fluorescens was tested for efficacy against culex quinquefasciatus larvae and pupae. at application rates of 100, 200, 300 ml/m2, the formulation caused 100% elimination of larvae and pupae at day 1 after treatments and >80% reduction in pupal density for periods of 7, 12 and 11 days in cesspits and 5, 9 and 10 days in u-shaped drains. in both the habitats, the efficacy of the formulation agains ...200312875927
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