[detection of plague microbe in the fleas by polymerase chain reaction by using magnetic immunosorbents].the polymerase chain reaction (pcr) with and without pre-treatment of the samples of the fleas citellophilus tesquorum with magnoimmunosorbents was used to detect the plague microbe in the samples containing 142, 168, 193 or more bacterial cells. pcr analysis reduced the time of tests of ectoparasites for plague to 6 hours. the use of a combination of pcr and magnoimmunosorbent simplified the most time-consuming and longest stage of isolation of plague microbial dna from the suspensions of the f ...200415042746
[relationships of parasitic triad co-members in a tuva natural plague focus: environmental and physiological aspects (experimental studies)].long-term experimental studies of the environmental and physiological aspects of relationships of parasitic triad co-members, performed in a tuva natural plague, by using citellophilus tesquorum altaicus fleas, their natural feeder--a long-tailed siberian souslik (citellus undulatus), and the yersinia pestis strain i-3226 that is typical of the focus were analyzed. a complex of biotic (the environmental features of a causative agent, avector, and a carrier) and abiotic (air temperature and moist ...200415689135
[conservation of yersinia pestis in winter by citellophilus tesquorum altaicus females and males].intersexual differences have been established in citellophilus tesquorum altaicus ioff (1936) from a tuva natural focus in the accumulation of yersinia pestis in fleas in autumn and its conservation during winter. the ectoparasites were infected and fed on the natural feeder - long-tailed siberian souslik (citellus undulates). small wild animals and insects were infected with the strain yersinia pestis 1-3226 typical of the focus. in winter fleas survived without the feeder under artificially cr ...200718277419
[relations of the causative agents of plague and listeriosis during their simultaneous stay in the flea citellophilus tesquorum at different environmental temperatures].experiments were carried out to determine the persistence of a plague microbe with varying praline labeling (isolated in the central caucasian mountain focus of plague in the fleas citellophilus tesquorum of a mountain subspecies) in the contamination of the mixed culture of the plague microbe and listeria. inoculation of fleas with a suspension of a plague microbe and listeriae demonstrates the manifest signs of antagonism between both species of microorganisms. this is evidenced by the reducti ...200818368716
[relationships of the plague pathogen and vector from different parasitic systems].the specific features of interaction of the strains of the plague microbe of the main subspecies, which circulate in the area of natural foci of mongolia and china, with citellophilus tesquorum sungaris fleas, the major vector of the plague pathogen in a transbaikalian natural focus, as well as with xenopsylla cheopis ones, the classical vector, were revealed. experiments used virulent yersinia pestis strains, such as i-3230 isolated from c.tesquorum in mongolia in 1998 and 2155 isolated from hu ...201323805481
[reservation forms of plague infectious agent in tuva natural focus].data characterizing the reservation forms of plague infectious agent in tuva natural focus are presented in the review. yersinia pestis was shown to persist most of the year in citellophilus tesquorum altaicus imago --the main carrier, getting into the animal organism only for a short time. an increased ability to aggregate in autumn and accumulate in clumps of c. tesquorum altaicus females that are more adapted to survive the cold season compared with males promote the persistence of the microo ...201223163049
[relationship of the flea citellophilus tesquorum altaicus (siphonaptera: ceratophyllidae) with the plague agent].some aspects of relationships of the flea citellophilus tesquorum altaicus and bacterium yersinia pestis of two strains isolated from different parts of the tuva natural plague focus were studied. peculiarities of elimination and blood meal activity of fleas infected with two strains of the plague agent were not revealed. differences in mortality and alimentary activity are considerably determined by the sex of insects. the ability of examined strains to form a proventriculus block was not ident ...200415174390
[the variability of yersinia pestis in the body of the flea].changes in the morphology and ultrastructure of y.pestis cells at different periods of their stay in the body of fleas (citellophilus tesquorum altaicus) have been studied. the study, carried out by means of optical and electron microscopy, as well as with the use of a culture medium for the isolation of l-forms, has revealed that in the body of fleas y.pestis cells undergo the effect of processes leading to their l-transformation. as the result of l-transformation, the infective agent may take ...19947941859
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