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identification and characterization of plasmids from the western aster yellows mycoplasmalike organism.supercoiled double-stranded dna molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (ay-mlo) by using cesium chloride-ethidium bromide density gradients. southern blot analysis, using plasmids from the severe strain of ay-mlo (say-mlo) as the probe, identified at least four plasmids in celery, aster, and periwinkle plants and in macrosteles severini leafhopper vectors infected with either the dwarf ay-mlo, tulela ...19902307660
association of beet leafhopper (hemiptera: cicadellidae) with a clover proliferation group phytoplasma in columbia basin of washington and oregon.at least 16 taxa of cicadellids and delphacids were tested by polymerase chain reaction (pcr) for the presence of a phytoplasma in the clover proliferation group, designated 16srvi. nucleic acid extracts from individual insects or groups of 5-10 were tested using pcr primers designed from the dna sequence of 16s-23s rrna or ribosomal protein genes of the pathogen. the beet leafhopper, circulifer tenellus (baker), was most often associated with the phytoplasma, with approximately 16% of the insec ...200515889714
incidence of the beet leafhopper-transmitted virescence agent phytoplasma in local populations of the beet leafhopper, circulifer tenellus, in washington state.phytoplasma diseases are increasingly becoming important in vegetable crops in the pacific northwest. recently, growers in the columbia basin and yakima valley experienced serious outbreaks of potato purple top disease that caused significant yield loss and a reduction in tuber processing quality. it was determined that the beet leafhopper-transmitted virescence agent (bltva) phytoplasma was the causal agent of the disease in the area and that this pathogen was transmitted by the beet leafhopper ...201020578882
comparative analysis of the plasmids from two isolates of "candidatus phytoplasma australiense".two plasmids from the plant-pathogenic mollicute "candidatus phytoplasma australiense" were completely sequenced from two isolates derived from different plant hosts. plasmid ppaph2 (3607bp) was obtained from phormium showing phormium yellow leaf symptoms and ppasb11 (3635bp) from strawberry showing strawberry lethal yellows symptoms. the plasmids varied in their copy number and nucleotide sequence yet contained the same four open reading frames (orfs). the deduced amino acid sequence derived fr ...200616620976
beet leafhopper (hemiptera: cicadellidae) settling behavior, survival, and reproduction on selected host plants.experiments were conducted to determine the settling behavior, survival, and reproduction of the beet leafhopper, circulifer tenellus (baker), when maintained on selected host plants. this leafhopper was recently identified in the columbia basin of washington and oregon as the probable vector of the beet leafhopper-transmitted virescence agent phytoplasma, causal agent of several vegetable crop diseases, including potato purple top. plants selected for study were sugar beet, beta vulgaris l.; ra ...200516539100
sequence analysis of two plasmids from the phytoplasma beet leafhopper-transmitted virescence agent.the complete nucleotide sequences of the two plasmids from the phytoplasma beet leafhopper-transmitted virescence agent (bltva) have been determined. the larger plasmid, pbltva-1, was 10 785 nt in length and contained 11 putative orfs, almost all of which were duplicated or triplicated on the plasmid due to the presence of large repeated regions. the sequence contained a series of tandem repeats, the largest of which was 338 nt long. the sequences of orfs 4 and 11 showed homology with the replic ...200415184567
beet leafhopper (hemiptera: cicadellidae) transmits the columbia basin potato purple top phytoplasma to potatoes, beets, and weeds.experiments were conducted to determine whether the beet leafhopper, circulifer tenellus (baker) (hemiptera: cicadellidae), transmits the purple top phytoplasma to potato, solanum tuberosum l.; beets, beta vulgaris l.; and selected weed hosts. the beet leafhopper-transmitted virescence agent (bltva) phytoplasma was identified as the causal agent of the potato purple top disease outbreaks that recently occurred in the columbia basin of washington and oregon. the phytoplasma previously was found t ...200616686123
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