| evagreen real-time pcr protocol for specific 'candidatus phytoplasma mali' detection and quantification in insects. | in this paper the validation and implementation of a real-time pcr protocol based on ribosomal protein genes has been carried out for sensitive and specific quantification of 'candidatus (ca.) phytoplasma mali' (apple proliferation phytoplasma, app) in insects. the method combines the use of evagreen(®) dye as chemistry detection system and the specific primer pair rpap15f-mod/rpap15r3, which amplifies a fragment of 238 bp of the ribosomal protein rplv (rpl22) gene of app. primers specificity wa ... | 2013 | 23474195 |