| reconstitution of authentic nanovirus from multiple cloned dnas. | we describe a new plant single-stranded dna (ssdna) virus, a nanovirus isolate originating from the faba bean in ethiopia. we applied rolling circle amplification (rca) to extensively copy the individual circular dnas of the nanovirus genome. by sequence analyses of more than 208 individually cloned genome components, we obtained a representative sample of eight polymorphic swarms of circular dnas, each about 1 kb in size. from these heterogeneous dna populations after rca, we inferred consensus ... | 2009 | 19656882 |
| circulative nonpropagative aphid transmission of nanoviruses: an oversimplified view. | plant virus species of the family nanoviridae have segmented genomes with the highest known number of segments encapsidated individually. they thus likely represent the most extreme case of the so-called multipartite, or multicomponent, viruses. all species of the family are believed to be transmitted in a circulative nonpropagative manner by aphid vectors, meaning that the virus simply crosses cellular barriers within the aphid body, from the gut to the salivary glands, without replicating or e ... | 2015 | 26178991 |