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plasmodium berghei ookinetes induce nitric oxide production in anopheles pseudopunctipennis midguts cultured in vitro.the anopheles pseudopunctipennis nitric oxide synthase gene (apnos) was identified and its partial sequence showed high homology with nos from a. stephensi, a. gambiae (putative sequence), and drosophila melanogaster. apnos was mainly expressed in male and female adult mosquitoes and was induced by a blood meal. nitric oxide (no) was produced by in vitro-cultured mosquito midguts inoculated by enema with plasmodium berghei ookinetes, saccharomyces cerevisiae, gram-positive bacteria (micrococcus ...200415350609
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