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transovarial transmission of st. louis encephalitis virus by culex pipiens complex mosquitoes.experiments were conducted to determine whether transovarial transmission (tot) of st. louis encephalitis (sle) virus occurs in culex pipiens complex mosquitoes, the principal vectors of sle virus in the central-eastern united states. in 1978, field-collected mosquitoes from memphis, tennessee, and mcleansboro, illinois, were used; during 1979, colonized mosquitoes from chicago, illinois, and memphis, tennessee, were used. mosquitoes were infected by feeding on viremic chicks inoculated with an ...19816266266
susceptibility of aedes albopictus c6/36 cells to viral infection.the susceptibility of the c6/36 clone of aedes albopictus monolayer cell cultures was determined with 46 prototype viruses passed through three subcultures. viral growth was confirmed by titration of the passage material in other susceptible host systems. nineteen viruses demonstrated good growth in c6/36 cells: coxsackievirus group a type 10 and group b types 2, 3, 4, and 5; enterovirus 69; mumps virus; poliovirus types 1 to 3; reovirus types 1 to 3; vaccinia virus; dengue virus type 2; eastern ...19873611315
establishment and characterization of st louis encephalitis virus persistent infections in aedes and culex mosquito cell lines.persistent infections with st louis encephalitis (sle) virus were established in three mosquito cell lines (aedes albopictus, a. dorsalis and culex tarsalis) and were maintained for over 2 years. all three persistently infected cell cultures shared two features: (i) no overt cytopathic effect and (ii) a relatively high proportion of cells infected (41 to 85%). the aedes persistently infected cultures were resistant to superinfection with the homologous virus but not heterologous viruses. two sig ...19882842432
vector competence of aedes albopictus from pine bluff, arkansas, for a st. louis encephalitis virus strain isolated during the 1991 epidemic.the vector competence of aedes albopictus from pine bluff, ar, was assessed for a st. louis encephalitis (sle) virus strain isolated during the 1991 epidemic. aedes albopictus were fed on hamsters with viremia levels of 10(4.6)-10(4.9) vero cell plaque-forming units (pfu)/ml. at 7 and 15 days postbloodfeeding, transmission trials were conducted using individual suckling mice. three of 313 ae. albopictus were determined to be infected with sle virus with titers of 10(6.3)-10(7.0) pfu/mosquito. at ...19947707054
phenotypes of st louis encephalitis virus mutants produced in persistently infected mosquito cell cultures.viral mutants that appeared during long-term persistent infections of mosquito cell cultures (aedes albopictus, a. dorsalis and culex tarsalis) with st louis encephalitis virus were characterized. evidence was obtained for the presence of temperature-sensitive mutants in the a. dorsalis and c. tarsalis persistently infected cultures, and small plaque mutants were predominant in all cultures except one of two cell cultures of c. tarsalis. virus from persistently infected a. albopictus cell cultur ...19882842433
nesting bird "host funnel" increases mosquito-bird contact rate.increases in vector-host contact rates can enhance arbovirus transmission intensity. we investigated weekly fluctuations in contact rates between mosquitoes and nesting birds using the recently described nest mosquito trap (nmt). the number of mosquitoes per nestling increased from < 1 mosquito per trap night to 36.2 in the final 2 wk of the nesting season. our evidence suggests the coincidence of the end of the avian nesting season and increasing mosquito abundances may have caused a "host funn ...023540137
effect of rearing temperature on transovarial transmission of st. louis encephalitis virus in mosquitoes.female aedes albopictus and aedes epactius infected by inoculation with st. louis encephalitis (sle) virus transmitted virus transovarially to their f1 progeny. with ae. epactius, minimal infection rates (mir) in f1 larvae were similar for progeny reared at 18 (+/- 1) degree c or 27 (+/- 1) degree c, whereas, mir's were significantly higher in f1 adults reared at 18 degrees c, than they were in f1 adults reared at 27 degrees c. use of different strains of sle virus appeared to influence transova ...19807435796
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