replication of mayaro virus in aedes albopictus cells: an electron microscopic study.the replication of mayaro virus in aedes albopictus cells, was studied by electron microscopy at various times post-infection. in infected cells we observed the presence of cytoplasmic vesicles containing viral nucleocapsids and mature virus particles but at no time did we detect virus budding into such vacuoles. budding of virus through plasma membrane was rarely observed. our results are discussed considering the possibility of the release of virus particles to the extracellular space by exocy ...19892539797
studies on the replication of mayaro virus grown in interferon treated cells.mayaro virus grown in interferon treated infected cells has been characterized with regard to its ability to replicate in vertebrate (tc7) and invertebrate (aedes albopictus) cells. virus purified from interferon treated tc7 cells adsorbs and penetrates to the same extent as the control virus. during infection, these virus particles caused inhibition of host protein synthesis and synthesized the same spectrum of viral proteins as normal virus. this population however, was apparently more sensiti ...19948524064
basic surface properties of aedes albopictus cells: effect of mayaro virus infection on electrostatic charge and surface tension.aedes albopictus cells possess a negative cell surface charge of -12.7 mv with an isoelectrophoretic point (iep) located between ph 3.0 and 4.0. infection with mayaro virus rendered the surface of a. albopictus cells less negative reaching a zeta-potential value of -9.7 mv after 100 h of infection. concomitantly, the iep of the infected cells were also altered from 3.0-4.0 to 4.0-5.0. furthermore, the contact angle measurements clearly showed qualitative alterations in the cell surface of infect ...19979722418
effect of brefeldin a on mayaro virus replication in aedes albopictus and vero cells.brefeldin a (bfa), a fungal metabolite that blocks transport of newly synthesized proteins from the endoplasmic reticulum, was found to inhibit mayaro virus replication. at the concentration of 0.05 microgram/ml, the yield of the virus was inhibited by 94% in aedes albopictus cells and by 99.5% in vero cells. treatment of a. albopictus cells with bfa did not inhibit the virus protein synthesis. however, this compound drastically reduced viral protein synthesis in vero cells. the inhibitory effec ...199910825924
effect of monensin on mayaro virus replication in monkey kidney and aedes albopictus cells.the effect of a cationic ionophore, monensin, on the replication of mayaro virus in monkey kidney tc7 and aedes albopictus cells has been studied. treatment of these cells with 1 micromol/l monensin during infection did not affect the virus protein synthesis but inhibited severely the virus replication. electron microscopy of the cells infected with mayaro virus and treated with monensin revealed that the morphogenesis of mayaro virus was impaired in tc7 but not in a. albopictus cells.200314524478
[mayaro: a re-emerging arbovirus in venezuela and latin america].mayaro virus produces nonspecific, sublethal disease symptoms, often confused with dengue, but with symptoms of arthalgias that can cause incapacitating disability. outbreaks have been localized and sporadic in the pan-amazonia forest since its first isolation in 1954 (trinidad and tobago). the literature available is scarce, diverse and dispersed. mayaro virus is an alphavirus, phylogenetically related to the semliki forest antigenic complex. una and mayaro viruses are the only viruses of this ...201223242303
a case of mayaro virus infection imported from french guiana.emergence of arboviruses is a rising problem in several areas in the world. here we report a case of mayaro virus infection that was diagnosed in a french citizen presenting a dengue-like syndrome with prolonged arthralgia following a travel in french guiana. diagnosis was based on serological testing, a newly developed specific rt-pcr and sequencing. the real incidence of this viral infection among travelers is poorly known but this case is the first reported in a european area where aedes albo ...201626921736
distribution of mayaro virus rna in polysomes during heat shock.mayaro virus (alphavirus) infection of aedes albopictus cells results in inhibition of cell protein synthesis and viral proteins are preferably synthesized. when infected cells are heat shocked, however, there is also an inhibition of viral protein synthesis, and there is preferential synthesis of heat shock proteins. based on these observations, the distribution of mayaro viral rna in polysomes and the association of p34 (capsid protein) with ribosomal fractions of the cells under such conditio ...19979175251
identification of mayaro virus nucleocapsid protein in nucleus of aedes albopictus cells.the modifications in the pattern of nuclear proteins of aedes albopictus cells in response to mayaro virus infection were analysed early and late after infection. the viral capsid (c) protein of 34 kda (p34) could be detected in the nuclear compartment 4 h after infection, soon after its synthesis in the cytoplasm. in addition to p34, a group of high molecular weight proteins was also present in this compartment late after infection. the exposition of infected cells to supra optimal temperature ...19979037738
metabolic and morphological changes in a. albopictus cells infected with mayaro virus under heat-shock conditions.we addressed the question how temperature elevation inhibits mayaro virus replication in aedes albopictus infected cells. the morphology and macromolecular changes induced by temperature, infection and high serum concentration were investigated in these cells. cells incubated with 2 and 10% serum at 28 degrees c disclosed an intense vacuolization and inhibition of [35s]methionine incorporation in a time-dependent manner. 34 and 50 kda viral structural proteins were detected 24 h after infection. ...19938392316
mayaro virus proteins.mayaro virus was grown in bhk-21 cells and purified by centrifugation in a potassium-tartrate gradient (5-50%). the electron microscopy analyses of the purified virus showed an homogeneous population of enveloped particles with 69 +/- 2.3 nm in diameter. three structural virus proteins were identified and designated p1, p2 and p3. their average molecular weight were p1, 54 kda; p2, 50 kda and p3, 34 kda. in mayaro virus infected aedes albopictus cells and in bhk-21 infected cells we detected six ...19938107591
effect of high temperature on aedes albopictus cells infected with mayaro virus.the multiplication of mayaro virus in aedes albopictus cells was drastically inhibited after incubation at 37 degrees c. the effect of short-term exposure of infected cells to high temperatures (heat shock) produced a preferential translation of the heat shock messengers when compared to the viral mrnas. when cells were shifted back to 28 degrees c (the optimum growth temperature for aedes albopictus cells), preferential translation of viral mrna occurred. although the infected cells were progra ...19872843253
prostaglandin a1 inhibits replication of mayaro virus in aedes albopictus cells.prostaglandin a1 (pga1) reduced mayaro virus replication in aedes albopictus (mosquito) cells in culture. the highest nontoxic dose of pga1, 7.5 microm, decreased virus production by 90%. in mayaro virus-infected cells, pga1 inhibited virus-specific protein synthesis. however, in mock-infected cells the presence of pga1 stimulated the synthesis of several proteins with molecular masses of 70, 57 and 23 kda, respectively. the data obtained from this study show that pga1 plays a role in the metabo ...19957581025
serum concentration and increased temperature alter mayaro virus rna and protein synthesis in aedes albopictus (mosquito)-infected cells.we have previously shown the inhibition of mayaro virus multiplication in aedes albopictus-infected cells maintained at a supraoptimal temperature for growth (37 degrees c) and a stimulation of virus production in response to high serum concentrations in the incubation medium. in the present study, we addressed the question of how the effect of continuous heat stress and high serum concentration soon after infection interfere with virus macromolecule synthesis. cells maintained at 28 degrees c i ...19957581023
present and future arboviral threats.arthropod-borne viruses (arboviruses) are important causes of human disease nearly worldwide. all arboviruses circulate among wild animals, and many cause disease after spillover transmission to humans and agriculturally important domestic animals that are incidental or dead-end hosts. viruses such as dengue (denv) and chikungunya (chikv) that have lost the requirement for enzootic amplification now produce extensive epidemics in tropical urban centers. many arboviruses recently have increased i ...201019857523
modulation in response to temperature of mayaro virus proteosynthesis in aedes albopictus cells.incubation of aedes albopictus cells infected with mayaro virus at 37 degrees c causes inhibition of virus replication. during the first hour post infection (p.i.) incubation at 37 degrees c inhibited cellular and virus proteosynthesis. a preferential translation of heat shock proteins 82 kd and 70 kd was observed. after incubations longer than 1 hr at 37 degrees c, a switch to normal pattern of cell protein synthesis occurred without recovery of virus proteosynthesis. in addition, preferential ...19892570510
ultrastructural study of mayaro virus replication in bhk-21 cells.the replication of mayaro virus in bhk-21 cells was studied by electron microscopy. the infected cells show an intense vacuolization and proliferation of membranous structures. at 5 h post-infection, precursor virus particles were seen in the cytoplasm of infected cells. later, mature virus particles were found outside the cells and budding from the plasma membrane. enveloped virus particles were also observed inside the vesicles and budding across their membrane. the release of virus particles ...19902173526
effect of heat shock on gene expression of aedes albopictus cells infected with mayaro virus.three major mayaro virus proteins of 62, 50 and 34 kda were detected in aedes albopictus cells after 48 h postinfection at 28 degrees c. when the infected cells were shifted from 28 to 37 degrees c for 90 min (heat shock conditions), the synthesis of two major heat shock proteins (hsp) 82 and 70 kda was induced concomitantly with strong inhibition of virus and normal protein synthesis. total cellular rna was isolated from mock and infected cells incubated at 28 degrees c or under heat shock. nor ...19911711236
effect of hypertonic medium on the protein synthesis in l-a9 and aedes albopictus cells infected with mayaro virus. 19911668789
inhibition of mayaro virus replication by cerulenin in aedes albopictus cells.the antibiotic cerulenin, an inhibitor of lipid synthesis, was shown to suppress mayaro virus replication in aedes albopictus cells at non-cytotoxic doses. cerulenin blocked the incorporation of [3h]glycerol into lipids when present at any time post infection (p.i.). cerulenin added at the beginning of infection inhibited the synthesis of virus proteins. however, when this antibiotic was added at later stages of infection, it had only a mild effect on the virus protein synthesis. the possibility ...199810358744
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