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identification of brazilian flaviviruses by a simplified reverse transcription-polymerase chain reaction method using flavivirus universal primers.we report a simplified reverse transcription-polymerase chain reaction (rt-pcr) method for identification of brazilian flaviviruses based on the patterns of electrophoretic separation of the amplicons. the rt-pcr was done on the culture fluids of aedes albopictus c6/36 cells infected with brazilian flaviviruses, without previous extraction of viral rna, using flavivirus universal primers that anneal to highly conserved sequences within the nonstructural protein 5 and 3'- non translated region of ...19989749625
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