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three distinct mechanisms facilitate genetic isolation of sympatric wheat streak mosaic virus lineages.cross-protection and vector transmission bottlenecks have been proposed as mechanisms facilitating genetic isolation of sympatric viral lineages. molecular markers were used to monitor establishment and resolution of mixed infections with genetically defined strains of wheat streak mosaic virus (wsmv). two closely related wsmv strains from the u.s. (type and sidney 81) exhibited reciprocal cross-protection in wheat, confirming this classic phenomenon as a mechanism of genetic isolation. in contr ...200111289805
colony establishment and maintenance of the eriophyid wheat curl mite aceria tosichella for controlled transmission studies on a new virus-like pathogen.high plains disease (hpd) is of serious economic concern for wheat and corn production, but little is known about the virus-like causal agent. in the field, hpd is often associated with wheat streak mosaic virus (wsmv) and both pathogens are transmitted by the same eriophyid wheat curl mite, aceria tosichella keifer. the objective of this study was to develop methods for establishing and maintaining hpd-transmitting wheat curl mite colonies for their use in studies on hpd. towards this goal, mit ...200312565164
molecular cytogenetic discrimination and reaction to wheat streak mosaic virus and the wheat curl mite in zhong series of wheat--thinopyrum intermedium partial amphiploids.thinopyrum intermedium (2n = 6x = 42, jjjsjsss) is potentially a useful source of resistance to wheat streak mosaic virus (wsmv) and its vector, the wheat curl mite (wcm). five partial amphiploids, namely zhong 1, zhong 2, zhong 3, zhong 4, and zhong 5, derived from triticum aestivum x thinopyrum intermedium crosses produced in china, were screened for wsmv and wcm resistance. zhong 1 and zhong 2 had high levels of resistance to wsmv and wcm. the other three partial amphiploids, zhong 3, 4, and ...200312669806
assessment of aegilops tauschii for resistance to biotypes of wheat curl mite (acari: eriophyidae).aegilops tauschii, the wild diploid d-genome progenitor of wheat, triticum aestivum l., is an important source of resistance to several arthropod pests and pathogens. a total of 108 ae. tauschii accessions from different geographic regions were evaluated for resistance to biotypes of the wheat curl mite, aceria tosichella keifer, from kansas, nebraska, and montana. the wheat curl mite is the only vector known to transmit wheat streak mosaic virus. wheat curl mite resistance was detected in germp ...200314503608
influence of volunteer wheat plant condition on movement of the wheat curl mite, aceria tosichella, in winter wheat.the wheat curl mite (wcm), aceria tosichella keifer, is the vector of wheat streak mosaic virus and high plains virus which cause significant crop loss in winter wheat throughout the western great plains. volunteer wheat emerging before harvest, as a result of severe hail, is the primary source of mites and virus that infect fall-planted winter wheat. wind-borne movement of the wcm is of key importance in the spread and infection of the virus complex. significant movement of wcm from wheat has b ...200314974690
plant virus hc-pro is a determinant of eriophyid mite transmission.the eriophyid mite transmitted wheat streak mosaic virus (wsmv; genus tritimovirus, family potyviridae) shares a common genome organization with aphid transmitted species of the genus potyvirus. although both tritimoviruses and potyviruses encode helper component-proteinase (hc-pro) homologues (required for nonpersistent aphid transmission of potyviruses), sequence conservation is low (amino acid identity, approximately 16%), and a role for hc-pro in semipersistent transmission of wsmv by the wh ...200515994799
nested deletion analysis of wheat streak mosaic virus hc-pro: mapping of domains affecting polyprotein processing and eriophyid mite transmission.a series of in-frame and nested deletion mutations which progressively removed 5'-proximal sequences of the wheat streak mosaic virus (wsmv) hc-pro coding region (1152 nucleotides) was constructed and evaluated for pathogenicity to wheat. wsmv hc-pro mutants with 5'-proximal deletions of 12 to 720 nucleotides systemically infected wheat. boundary sequences flanking the deletions were stable and unaltered by passage through plants for all deletion mutants except hcd12 (lacking hc-pro codons 3-6) ...200616540139
isolation, transmission and purification of the high plains virus.the wheat curl mite (aceria tosichella keifer) often simultaneously transmits the high plains virus and wheat streak mosaic virus under field conditions, resulting in doubly infected plants. in this study, a pure culture of the high plains virus (isolate hpv95id), which was infected with both high plains virus and wheat streak mosaic virus, was mechanically transmitted from barley (hordeum vulgáre l.) to maize (zea mays l.) by vascular puncture inoculation. different water temperatures and durat ...200616672165
substitution of conserved cysteine residues in wheat streak mosaic virus hc-pro abolishes virus transmission by the wheat curl mite.substitutions in the amino-proximal region of wheat streak mosaic virus (wsmv) hc-pro were evaluated for effects on transmission by the wheat curl mite (aceria tosichella keifer). alanine substitution at cysteine residues 16, 46 and 49 abolished vector transmission. although alanine substitution at cys(20) had no effect, substitution with arginine reduced vector transmission efficiency. random substitutions at other positions (lys(7) to asn, asn(19) to ile, and arg(45) to lys) did not affect vec ...200717680324
c-banding and fluorescence in situ hybridization studies of the wheat-alien hybrid 'agrotana'.'agrotana', a wheat-alien hybrid (2n = 56), is a potential source of resistance to common root rot, stem rust, wheat streak mosaic virus, and the wheat curl mite. however, the origin of 'agrotana', reported to be durum wheat x agropyron trichophorum (pubescent wheatgrass), is uncertain. the objective of this investigation was to determine the chromosome constitution of 'agrotana' using c-banding and fluorescence in situ hybridization techniques. the f1 hybrid of 'agrotana' x 'chinese spring' whe ...199418470093
fully biologically active in vitro transcripts of the eriophyid mite-transmitted wheat streak mosaic tritimovirus.abstract infectious rna of wheat streak mosaic virus (wsmv) has been produced using a full-length cdna clone as a template for in vitro transcription with sp6 rna polymerase. infectivity was dependent on the use of template plasmid dna that had not undergone spontaneous rearrangement during amplification in escherichia coli. the presence of wsmv in systemically infected wheat plants inoculated with in vitro transcripts was confirmed by reverse-transcription polymerase chain reaction of the wsmv ...199918944643
molecular markers indicate that the wheat curl mite, aceria tosichella keifer, may represent a species complex in australia.the wheat curl mite (wcm), aceria tosichella keifer, is an eriophyoid pest of cereals, and the vector responsible for transmitting wheat streak mosaic virus. several authors have suggested cryptic species of this mite identified through morphological variation, but this has never been conclusively demonstrated. here, we use the mitochondrial 16s rrna gene and two nuclear markers (internal transcribed spacer 1 and adenine nucleotide translocase) to show that wcm from australia consists of at leas ...200919224660
quantifying wheat yield across the field as a function of wheat streak mosaic intensity: a state space approach.wheat streak mosaic virus (wsmv), vectored by the wheat curl mite aceria tosichella, is one of the major limiting factors in wheat production in the texas panhandle. the mites are blown by wind into wheat fields from nearby volunteer wheat fields or fields supporting vegetation which harbor virulent mites. consequently, gradients of wheat streak severity are often observed stretching from the edges of wheat fields into the center or beyond. to describe the magnitude of the spatial relationships ...200919271985
thinopyrum ponticum and th. intermedium: the promising source of resistance to fungal and viral diseases of wheat.thinopyrum ponticum and th. intermedium provide superior resistance against various diseases in wheat (ttricum aestivum). because of their readily crossing with wheat, many genes for disease resistance have been introduced from the wheatgrasses into wheat. genes for resistance to leaf rust, stem rust, powdery mildew, barley yellow dwarf virus, wheat streak mosaic virus, and its vector, the wheat curl mite, have been transferred into wheat by producing chromosome translocations. these genes offer ...200919782957
wheat cultivar-specific disease synergism and alteration of virus accumulation during co-infection with wheat streak mosaic virus and triticum mosaic virus.triticum mosaic virus (trimv), the type member of the newly proposed poacevirus genus, and wheat streak mosaic virus (wsmv), the type member of tritimovirus genus of the family potyviridae, infect wheat naturally in the great plains and are transmitted by wheat curl mites. in this study, we examined the ability of these viruses to infect selected cereal hosts, and found several differential hosts between trimv and wsmv. additionally, we examined the interaction between wsmv and trimv in three wh ...201020128696
hairpin rna derived from viral nia gene confers immunity to wheat streak mosaic virus infection in transgenic wheat plants.wheat streak mosaic virus (wsmv), vectored by wheat curl mite, has been of great economic importance in the great plains of the united states and canada. recently, the virus has been identified in australia, where it has spread quickly to all major wheat growing areas. the difficulties in finding adequate natural resistance in wheat prompted us to develop transgenic resistance based on rna interference (rnai). an rnai construct was designed to target the nuclear inclusion protein 'a' (nia) gene ...201020374525
wheat curl mite resistance: interactions of mite feeding with wheat streak mosaic virus infection.the majority of plant viruses are dependent on arthropod vectors for spread between plants. wheat streak mosaic virus (family potyviridae, genus tritimovirus, wsmv) is transmitted by the wheat curl mite, aceria tosichella keifer, and this virus and vector cause extensive yield losses in most major wheat (triticum aestivum l.)-growing regions of the world. many cultivars in use are susceptible to this vector-virus complex, and yield losses of 10-99% have been documented. wheat curl mite resistanc ...201121882710
population genetics of the wheat curl mite (aceria tosichella keifer) in australia: implications for the management of wheat pathogens.the wheat curl mite (wcm), aceria tosichella keifer, is a polyphagous eriophyoid mite and the primary vector of wheat streak mosaic virus (wsmv) and five other viral pathogens in cereals. previous research using molecular markers and a series of laboratory experiments found a. tosichella in australia to consist of two genetically distinct lineages, which have broad overlapping distributions and differ in their ability to transmit wsmv under controlled conditions. this pattern of transmission als ...201122030277
multiple categories of resistance to wheat curl mite (acari: eriophyidae) expressed in accessions of aegilops tauschii.the wheat curl mite, aceria tosichella keifer, is an important pest in the western plains of the united states as well as in most major wheat-growing regions of the world. this mite is a vector of the economically important diseases wheat streak mosaic virus (wsmv), triticum mosaic virus (trimv), and high plains virus (hpv). this study looked at seven accessions of aegilops tauschii (coss) schmal to determine if they exhibit antibiosis, tolerance, and/or antixenosis to the wheat curl mite using ...201223356085
population dynamics of the wheat curl mite (acari: eriophyidae) during the heading stages of winter wheat.the wheat curl mite (aceria tosichella keifer) is the only known vector of three viruses in wheat-wheat streak mosaic virus, wheat mosaic virus, and triticum mosaic virus. the economic impact of this disease complex is linked to the presence of suitable hosts prior to winter wheat maturing in early summer and the movement of wheat curl mite from wheat to oversummering hosts prior to wheat harvest. previous research has documented the prevalence and density of mite populations on maturing wheat h ...201728334133
wheat genotypes with combined resistance to wheat curl mite, wheat streak mosaic virus, wheat mosaic virus, and triticum mosaic virus.the wheat curl mite, aceria tosichella keifer, (wcm) is a global pest of bread wheat that reduces yields significantly. in addition, wcm carries wheat streak mosaic virus (wsmv, family potyviridae, genus tritimovirus), the most significant wheat virus in north america; high plains wheat mosaic virus (hpwmov, genus emaravirus, formerly high plains virus); and triticum mosaic virus (trimv, family potyviridae, genus poacevirus). viruses carried by wcm have reduced wheat yields throughout the u.s. g ...201728087646
the effect of temperature, relative humidity, and virus infection status on off-host survival of the wheat curl mite (acari: eriophyidae).the wheat curl mite, aceria tosichella keifer, is an eriophyid pest of wheat, although its primary economic impact on wheat is due to the transmission of wheat streak mosaic (wsmv), wheat mosaic (also known as high plains virus), and triticum mosaic (trimv) viruses. these viruses cause significant annual losses in winter wheat production throughout the western great plains. temperature and humidity are factors that often influence arthropod survival, especially during dispersal from their hosts, ...201526470294
in vitro transcripts of wild-type and fluorescent protein-tagged triticum mosaic virus (family potyviridae) are biologically active in wheat.triticum mosaic virus (trimv) (genus poacevirus, family potyviridae) is a recently described eriophyid mite-transmitted wheat virus. in vitro rna transcripts generated from full-length cdna clones of trimv proved infectious on wheat. wheat seedlings inoculated with in vitro transcripts elicited mosaic and mottling symptoms similar to the wild-type virus, and the progeny virus was efficiently transmitted by wheat curl mites, indicating that the cloned virus retained pathogenicity, movement, and w ...201526214124
wheat curl mite, aceria tosichella, and transmitted viruses: an expanding pest complex affecting cereal crops.the wheat curl mite (wcm), aceria tosichella, and the plant viruses it transmits represent an invasive mite-virus complex that has affected cereal crops worldwide. the main damage caused by wcm comes from its ability to transmit and spread multiple damaging viruses to cereal crops, with wheat streak mosaic virus (wsmv) and wheat mosaic virus (wmov) being the most important. although wcm and transmitted viruses have been of concern to cereal growers and researchers for at least six decades, they ...201323179064
genetic characterization of north american populations of the wheat curl mite and dry bulb mite.the wheat curl mite, aceria tosichella keifer, transmits at least three harmful viruses, wheat streak mosaic virus (wsmv), high plains virus (hpv), and triticum mosaic virus (trimv) to wheat (triticum aestivum l.) throughout the great plains. this virus complex is considered to be the most serious disease of winter wheat in the western great plains. one component of managing this disease has been developing mite resistance in wheat; however, identification of mite biotypes has complicated deploy ...201223156180
molecular characterization of haynaldia villosa chromatin in wheat lines carrying resistance to wheat curl mite colonization.wheat-haynaldia villosa (l.) schur, hybrid lines were tested as potential sources of resistance to colonization by the wheat curl mite, the vector of wheat streak mosaic virus. two lines, add 6v-1 and sub 6v-1, were found to be mite-resistant. fluorescence in situ hybridization using total genomic dna, from h. villosa in the presence of unlabelled wheat dna, confirmed that add 6v-1 is a disomic wheat-h. villosa chromosome addition line. sub 6v-1 turned out to be a homoeologous wheat-h. villosa c ...199624162394
development of pcr markers linked to resistance to wheat streak mosaic virus in wheat.wheat streak mosaic virus (wsmv), vectored by the wheat curl mite (acer tulipae), is an important disease of wheat (triticum aestivum l.) in the north american great plains. resistant varieties have not been developed for two primary reasons. first, useful sources of resistance have not been available, and second, field screening for virus resistance is laborious and beyond the scope of most breeding programs. the first problem may have been overcome by the development of resistance to both the ...199624162306
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