simultaneous diagnosis of cetacean morbillivirus infection in dolphins stranded in the spanish mediterranean sea in 2011 using a novel universal probe library (upl) rt-pcr assay.a highly sensitive and specific real-time (rt) rt-pcr assay has been developed for rapid, simultaneous detection of three strains of cetacean morbillivirus (cemv). in this assay, two pcr primers and a hydrolysis probe from a commercially available universal probe library (upl) are used to amplify a highly conserved region within the fusion protein gene. rt-pcr is carried out on the same sample using two primer sets in parallel: one set detects the more virulent strains, dolphin morbillivirus (dm ...201323380457
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