cowdria ruminantium identified in amblyomma gemma using a dna probe pcs20.amblyomma gemma ticks were collected from wild animals on a 20,000 acre game ranch in a heartwater endemic area in kenya, close to nairobi. a. variegatum is the main vector of heartwater in kenya. e.a. lewis, 1947, in a one sentence report has implicated a. gemma to be a vector of heartwater without giving any details. adult a. gemma were collected from giraffe giraffa camelopardalis, hartebeest alcephalus busephalus, eland taurotragus oryx and ostrich struthio camelus during cropping exercises. ...19938134629
growth of cowdria ruminantium in tissue culture endothelial cell lines from wild african mammals.endothelial cell cultures were established from several wild african mammalian species. long-term cultures were established from three ruminants, stable antelope (hippotragus niger), buffalo (syncerus caffer), and eland (tragelaphus oryx), and from an omnivore, the bushpig (potamochoerus porcus). cowdria ruminanntium was isolated from plasma of clinically affected animals in these four cell lines and in bovine endothelial cells used routinely for c. ruminantium propagation. nineteen different st ...19989577776
demonstration of a carrier state for cowdria ruminantium in wild ruminants from africa.four wild african ruminants, eland (taurotragus oryx), giraffe (giraffa camelopardalis), kudu (tragephalus strepsiceros strepsiceros), and blue wildebeest (connochaetes taurinus), were experimentally infected with the rickettsia cowdria ruminantium, the tickborne agent causing heartwater in domestic ruminants. the infections were established, and c. ruminantium was transmitted to naive small ruminants by the vector amblyomma hebraeum when transmission attempts were made at days 128 (eland and wi ...19989706566
transmission of cowdria ruminantium by amblyomma gemma from infected african buffalo (syncerus caffer) and eland (taurotragus oryx) to sheep.two african buffalo (syncerus caffer), an eland (taurotragus oryx) and a waterbuck (kobus defassa) were intravenously inoculated with cowdria ruminantium (kiswani). amblyomma gemma nymphs were fed on the animals at 3 weekly intervals. jugular blood was also collected at 3 weekly intervals and inoculated into sheep. nymphal ticks that fed on one buffalo on days 16 and 37 and on the other buffalo on day 58 after infection transmitted the disease as adults to sheep. nymphs that were applied to the ...200111556617
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