high prevalence of west nile virus: a continuing risk in acquiring infection from a mosquito bite.the prevalence of west nile virus (wnv) was evaluated by diplex real-time rt-pcr assay for the years 2001-2005 in culex species of mosquitoes, several species of dead birds, and clinically suspected mammals collected in kansas. the analysis was performed using a taqman-based diplex real-time rt-pcr assay targeted against two regions of the wnv genome, envelope glycoprotein gene and 3' untranslated region. the assay aided in the accurate detection of wnv in mosquitoes at high prevalence for the y ...200617187569
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