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serological survey for lyme disease in sika deer (cervus nippon yesoensis) by enzyme-linked immunosorbent assay (elisa).seventy-six wild sika deer (cervus nippon yesoensis) from areas endemic for borrelia burgdorferi during 1988 to 1989 had the igg antibody to the bacteria in enzyme-linked immunosorbent assay. the percentage of seropositive deer was 25.0% to strain ho14 and 22.4% to strain hp3, respectively. specific igg antibody titers were high in summer but low in winter. in summer, seropositive deer were 75.0%. similar results were obtained in serum specimens obtained monthly from 4 farmed deer. it was sugges ...19911808467
detection of lyme disease spirochetes in the skin of naturally infected wild sika deer (cervus nippon yesoensis) by pcr.we demonstrated the presence of borrelia burgdorferi sensu lato dna in the skin tissues of naturally infected wild sika deer, using pcr. the risk of transmission of b. burgdorferi sensu lato is recognized in sika deer.19957747979
complement-mediated killing of borrelia garinii--bactericidal activity of wild deer serum.the susceptibility of borrelia garinii to fresh wild deer sera was determined by incubating strain sika2 at 10% serum concentration for 1 hr at 37 c in an in vitro bactericidal assay. each serum showed bactericidal effects at various levels. the effect was dependent on the concentration of antibody to the spirochetes. complement was essential in the bactericidal assay because the inactivated deer serum showed greatly decreased activity. our results suggest that b. garinii is sensitive to deer se ...19947854216
borrelia burgdorferi sensu lato in an endemic environment: wild sika deer (cervus nippon yesoensis) with infected ticks and antibodies.ticks and blood samples were collected from wild sika deer (cervus nippon yesoensis) during a hunting season (august to october) of 1991 at a selected location in hokkaido, japan. ixodes persulcatus (adult and nymph) and i. ovatus (adult) were the common ticks on sika deer. spirochetes were detected in the midgut of the ticks by the indirect peroxidase-conjugated antibody staining method and by dark-field microscopy after cultivation. by the reactive pattern of monoclonal antibodies, isolates we ...19968871523
ticks and antibodies to borrelia burgdorferi from mammals at cape hatteras, nc and assateague island, md and va.results of a survey for ixodid ticks and/or serum antibodies to borrelia burgdorferi from 14 species of small to large mammals from eastern coastal areas of the united states are presented. most samples were obtained from july 1987 through june 1989 (excluding december-march) at 3 locales: assateague is. national seashore, worcester co., md., and accomack co., va. (approximately 38 degrees 05' n 75 degrees 10' w), and cape hatteras national seashore, dare co., nc (approximately 35 degrees 30' n ...199910534951
complement-mediated killing of borrelia burgdorferi by nonimmune sera from sika deer.various species of cervid deer are the preferred hosts for adult, black-legged ticks (ixodes scapularis and ixodes pacificus) in the united states. although frequently exposed to the agent of lyme disease (borrelia burgdorferi), these animals, for the most part, are incompetent as transmission reservoirs. we examined the borreliacidal activity of normal and b. burgdorferi-immune sera from sika deer (cervus nippon) maintained in a laboratory setting and compared it to that of similar sera from re ...200011191897
identification and molecular survey of borrelia burgdorferi sensu lato in sika deer (cervus nippon) from jilin province, north-eastern china.lyme disease caused by borrelia burgdorferi sensu lato (s.l.) is a common disease of domestic animals and wildlife worldwide. sika deer is first-grade state-protected wildlife animals in china and have economic consequences for humans. it is reported that sika deer may serve as an important reservoir host for several species of b. burgdorferi s.l. and may transmit these species to humans and animals. however, little is known about the presence of borrelia pathogens in sika deer in china. in this ...201727818123
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