inhibition of protease-resistant prion protein formation in a transformed deer cell line infected with chronic wasting disease.chronic wasting disease (cwd) is an emerging transmissible spongiform encephalopathy (prion disease) of north american cervids, i.e., mule deer, white-tailed deer, and elk (wapiti). to facilitate in vitro studies of cwd, we have developed a transformed deer cell line that is persistently infected with cwd. primary cultures derived from uninfected mule deer brain tissue were transformed by transfection with a plasmid containing the simian virus 40 genome. a transformed cell line (mdb) was exposed ...200616378962
prion protein genes in caribou from alaska.prion protein genes were sequenced in free-ranging alaska caribou (rangifer tarandus grantii). caribou prion alleles are identical or nearly so to those of wapiti, white-tailed deer, and mule deer. five single-nucleotide polymorphisms were detected with substitutions at residues 2 (v-->m), 129 (g-->s), 138 (s-->n), 146 (n-->n), and 169 (v-->m). the 138n codon had been previously reported only in prion pseudogenes of other cervids. in caribou, the 138s and 138n alleles are present at frequencies ...200717495306
prp genotypes of free-ranging wapiti (cervus elaphus nelsoni) with chronic wasting disease.variation in prp prion gene sequence appears to modulate susceptibility to chronic wasting disease (cwd), a naturally occurring prion disease affecting four north american species of the family cervidae. wapiti (cervus elaphus nelsoni) prp is polymorphic at codon 132 [methionine (m) or leucine (l)]. we genotyped 171 samples, collected between 2002 and 2005 from cwd-infected and uninfected wapiti from three free-ranging populations in colorado, usa, to study influences of prp polymorphisms on cwd ...200818420812
genetic variability of the prion protein gene (prnp) in wild ruminants from italy and scotland.the genetics of the prion protein gene (prnp) play a crucial role in determining the relative susceptibility to transmissible spongiform encephalopathies (tses) in several mammalian species. to determine the prnp gene variability in european red deer (cervus elaphus), roe deer (capreolus capreolus) and chamois (rupicapra rupicapra), the prnp open reading frame from 715 samples was analysed to reveal a total of ten single nucleotide polymorphisms (snps). in red deer, snps were found in codons 15, ...200919461206
targeting hunter distribution based on host resource selection and kill sites to manage disease risk.endemic and emerging diseases are rarely uniform in their spatial distribution or prevalence among cohorts of wildlife. spatial models that quantify risk-driven differences in resource selection and hunter mortality of animals at fine spatial scales can assist disease management by identifying high-risk areas and individuals. we used resource selection functions (rsfs) and selection ratios (srs) to quantify sex- and age-specific resource selection patterns of collared (n = 67) and hunter-killed ...201324324876
in vitro prion protein conversion suggests risk of bighorn sheep (ovis canadensis) to transmissible spongiform encephalopathies.transmissible spongiform encephalopathies (tses) affect both domestic sheep (scrapie) and captive and free-ranging cervids (chronic wasting disease; cwd). the geographical range of bighorn sheep (ovis canadensis; bhs) overlaps with states or provinces that have contained scrapie-positive sheep or goats and areas with present epizootics of cwd in cervids. no tses have been documented in bhs, but the susceptibility of this species to tses remains unknown.201323938169
detection of prion protein in the cerebrospinal fluid of elk (cervus canadensis nelsoni) with chronic wasting disease using protein misfolding cyclic amplification.cerebrospinal fluid (csf) has been examined as a possible source for preclinical diagnosis of prion diseases in hamsters and sheep. the present report describes the detection of chronic wasting disease (cwd) in the csf of elk and evaluates its usefulness as an antemortem test for cwd. the csf from 6 captive and 31 free-ranging adult elk was collected at necropsy and evaluated for the presence of the abnormal isoform of the prion protein that has been associated with cwd (prp(cwd)) via protein mi ...201222621952
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