canine kobuvirus infections in korean investigate canine kobuvirus (cakov) infection, fecal samples (n = 59) were collected from dogs with or without diarrhea (n = 21 and 38, respectively) in the republic of korea (rok) in 2012. cakov infection was detected in four diarrheic samples (19.0 %) and five non-diarrheic samples (13.2 %). all cakov-positive dogs with diarrhea were found to be infected in mixed infections with canine distemper virus and canine parvovirus or canine adenovirus. there was no significant difference in the pr ...201424906525
co-circulation of the rare cpv-2c with unique gln370arg substitution, new cpv-2b with unique thr440ala substitution, and new cpv-2a with high prevalence and variation in heilongjiang province, northeast trace evolution of canine parvovirus-2 (cpv-2), a total of 201 stool samples were collected from dogs with diarrhea in heilongjiang province of northeast china from may 2014 to april 2015. the presence of cpv-2 in the samples was determined by pcr amplification of the vp2 gene (568 bp) of cpv-2. the results revealed that 95 samples (47.26%) were positive for cpv-2, and they showed 98.8%-100% nucleotide identity and 97.6%-100% amino acid identity. of 95 cpv-2-positive samples, types new2a (ser ...201526348721
co-circulation of canine coronavirus i and iia/b with high prevalence and genetic diversity in heilongjiang province, northeast trace the evolution of canine coronavirus (ccov), 201 stool samples from diarrheic dogs in northeast china were subjected to reverse transcription-polymerase chain reactions (rt-pcrs) targeting the partial m and s genes of ccov, followed by an epidemiological analysis. m gene rt-pcrs showed that 28.36% (57/201) of the samples were positive for ccov; of the 57 positive samples, ccov-i and ccov-ii accounted for 15.79% (9/57) and 84.21% (48/57), respectively. a sequence comparison of the partial ...201626771312
phylogeny and prevalence of kobuviruses in dogs and cats in the uk.the kobuviruses represent an emerging genus in the picornaviridae. here we have used next generation sequencing and conventional approaches to identify the first canine kobuvirus (cakov) from outside the usa. phylogenetic analysis suggests that a single lineage genotype of cakov now exists in europe and the usa with 94% nucleotide similarity in the coding region. cakov was only identified in a single case from a case-control study of canine diarrhoea, suggesting this virus was not a frequent cau ...201323490561
detection and genetic characterization of feline order to survey for feline kobuviruses infection, fecal samples (n = 39) of cats with diarrhea were collected during 2011-2012. six (14.5%) of the fecal samples tested were positive for feline kobuviruses. the partial nucleotide sequences of feline kobuviruses based on the rna-dependent rna polymerase gene were compared to those of other species. feline kobuviruses were most closely related to canine kobuvirus in terms of their amino acid and nucleotide levels. in a phylogenetic tree, feline ...201323963764
molecular detection and nucleotide sequence analysis of a new aichi virus closely related to canine kobuvirus in sewage samples.between 2001 and 2005, 207 raw sewage samples were collected at the inflow of a sewage treatment plant in aichi prefecture, japan. of the 207 sewage samples, 137 (66.2 %) were found to be positive for amplification of aichi virus (aiv) nucleotide using reverse transcription (rt)-pcr with 10 forward and 10 reverse primers in the 3d region corresponding to the nucleotide sequence of all kobuviruses. aiv genotype a sequences were detected in all 137 samples. new sequences of aiv were detected in ni ...201424523156
genetic characteristics of the complete feline kobuvirus genome.we sequenced the complete genome of a feline kobuvirus and determined relationships with other kobuviruses. this kobuvirus has an 8,269-nucleotide-long rna genome, excluding the poly(a) tail. the genome contains a 7,311-bp open reading frame (orf) encoding a putative polyprotein precursor of 2,437 amino acids, a 717-bp 5'-untranslated region (utr), and a 241-bp 3'-utr. the l protein sequence was found to be the most variable region in the feline kobuvirus genome. interestingly, the 5'-utr b and ...201525404141
epidemiology of human and animal kobuviruses.kobuviruses are member of the family picornaviridae. initially, members in kobuvirus genus were named according to the basis of their host species. the viruses found in humans called "aichi virus", the viruses from cattle called "bovine kobuvirus", and the viruses isolated from pigs called "porcine kobuvirus". currently, taxonomy of kobuviruses has been proposed and the virus species have been renamed. the "aichi virus" has been renamed as "aichivirus a", "bovine kobuvirus" has been renamed as " ...201425674585
novel picornavirus in domestic rabbits (oryctolagus cuniculus var. domestica).picornaviruses (family picornaviridae) are small, non-enveloped viruses with positive sense, single-stranded rna genomes. the numbers of the novel picornavirus species and genera are continuously increasing. picornaviruses infect numerous vertebrate species from fish to mammals, but have not been identified in a member of the lagomorpha order (pikas, hares and rabbits). in this study, a novel picornavirus was identified in 16 (28.6%) out of 56 faecal samples collected from clinically healthy rab ...201626588888
a distinct class of internal ribosomal entry site in members of the kobuvirus and proposed salivirus and paraturdivirus genera of the picornaviridae.the 5'-untranslated regions (5' utrs) of picornavirus genomes contain an internal ribosomal entry site (ires) that promotes the end-independent initiation of translation. picornavirus iress are classified into four structurally distinct groups, each with different initiation factor requirements. here, we identify a fifth ires class in members of kobuvirus, salivirus, and paraturdivirus genera of picornaviridae: aichi virus (av), bovine kobuvirus (bkv), canine kobuvirus (ckov), mouse kobuvirus (m ...201122114340
Characterization of a canine homolog of human Aichivirus.Many of our fatal "civilization" infectious diseases have arisen from domesticated animals. Although picornaviruses infect most mammals, infection of a companion animal is not known. Here we describe the identification and genomic characterization of the first canine picornavirus. Canine kobuvirus (CKoV), identified in stool samples from dogs with diarrhea, has a genomic organization typical of a picornavirus and encodes a 2,469-amino-acid polyprotein flanked by 5' and 3' untranslated regions. C ...201121880761
detection of kobuvirus rna in japanese domestic investigate whether kokuvirus is present in japanese dogs, we examined the fecal samples obtained from 94 diarrheal household dogs and 50 clinically healthy kenneled dogs by rt-pcr. the gene was detected in 37.2% and 48.0% in the former and the latter, respectively, suggesting that canine kobuvirus (cakov) is circulating among japanese dogs. from the result of the latter, however, cakov may not be a primary pathogen. furthermore, all gene-positive dogs were purebreds aged four months or young ...201627488907
molecular characterization of new described kobuvirus in dogs with diarrhea in china.canine kobuvirus (cakvs) was a newly described virus detected in dogs in the us and italy. to learn more about cakvs, 5 of 106 fecal samples from diarrhea dogs were positive with cakvs in china, and the full genome of cakvs strain ch-1 isolated from dog with diarrhea was sequenced. the genome consists of 8186 nucleotides, excluding the 3' poly (a) tail, and an open reading frame that maps between nucleotide positions 601 and 7943 which encodes a 2446 amino acid polyprotein. based on the complete ...201627995024
prevalence and genomic characteristics of canine kobuvirus in southwest investigate canine kobuvirus (cakov) infection in southwest china, 107 fecal samples were collected from dogs with obvious diarrhea in sichuan and chongqing regions, china. cakov infection was detected in 54 diarrheic samples (50.46%) by rt-pcr targeting a partial fragment (504 bp) of the 3d gene. comparison of these partial 3d gene sequences from 14 of these cakov-positive samples show 95.4%-99.0% nucleotide (nt) identity within this group, and nt identities ranging from 93.1% to 98.2% with ...201829143141
one-step triplex pcr/rt-pcr to detect canine distemper virus, canine parvovirus, and canine rapidly distinguish canine distemper virus (cdv), canine parvovirus (cpv), and canine kobuvirus (cakov) in practice, a one-step multiplex pcr/rt-pcr assay was developed, with detection limits of 102.1 tcid50 for cdv, 101.9 tcid50 for cpv and 103 copies for cakov. this method did not amplify nonspecific dna or rna from other canine viruses. therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of cdv, cpv and cakov in dogs.201829367517
viruses in diarrhetic dogs include novel kobuviruses and sapoviruses.the close interactions of dogs with humans and surrounding wild life provide frequent opportunities for cross-species virus transmissions. in order to initiate an unbiased characterization of the eukaryotic viruses in the gut of dogs we used deep sequencing of partially purified viral-capsid protected nucleic acids from the feces of eighteen diarrhetic dogs. we identified known canine parvoviruses, coronaviruses, and rotaviruses, and characterized the genomes of the first reported canine kobuvir ...201121775584
extra-intestinal detection of canine kobuvirus in a puppy from southern brazil.this study presents the pathological, immunohistochemical, and molecular findings associated with the extra-intestinal detection of canine kobuvirus (cakv) in a 5-month-old chihuahua puppy, that had a clinical history of bloody-tinged feces. principal pathological findings were interstitial pneumonia, necrotizing bronchitis, and parvovirus-induced enteritis. molecular diagnostic methods identified cakv within the cerebellum, cerebrum, lung, tonsil, and liver. cakv and rotavirus were not identifi ...201727888408
molecular characterization of canine kobuvirus in wild carnivores and the domestic dog in africa.knowledge of kobuvirus (family picornaviridae) infection in carnivores is limited and has not been described in domestic or wild carnivores in africa. to fill this gap in knowledge we used rt-pcr to screen fresh feces from several african carnivores. we detected kobuvirus rna in samples from domestic dog, golden jackal, side-striped jackal and spotted hyena. using next generation sequencing we obtained one complete kobuvirus genome sequence from each of these species. our phylogenetic analyses r ...201525667111
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