structure and cell surface maturation of the attachment glycoprotein of human respiratory syncytial virus in a cell line deficient in o glycosylation.the synthesis of the extensively o-glycosylated attachment protein, g, of human respiratory syncytial virus and its expression on the cell surface were examined in a mutant chinese hamster ovary (cho) cell line, ldld, which has a defect in protein o glycosylation. these cells, used in conjunction with an inhibitor of n-linked oligosaccharide synthesis, can be used to establish conditions in which no carbohydrate addition occurs or in which either n-linked or o-linked carbohydrate addition occurs ...19892677404
a cowpox virus gene required for multiplication in chinese hamster ovary cells.cowpox virus, in contrast to vaccinia virus, can multiply in chinese hamster ovary cells. to study the genetic basis for this difference in host range, recombinants between vaccinia and cowpox viruses were isolated and their dna restriction patterns were examined. the ability to multiply in chinese hamster ovary cells could be correlated with the conservation of cowpox virus sequences mapping at the left end of the genome. this was further demonstrated by marker rescue of the host range phenotyp ...19882831390
delay of vaccinia virus-induced apoptosis in nonpermissive chinese hamster ovary cells by the cowpox virus chohr and adenovirus e1b 19k genes.the infection of vaccinia virus in chinese hamster ovary (cho) cells produces a rapid shutdown in protein synthesis, and the infection is abortive (r.r. drillien, d. spehner, and a. kirn, virology 111:488-499, 1978; d.e. hruby, d.l. lynn, r. condit, and j.r. kates, j. gen. virol. 47:485-488, 1980). cowpox virus, which can productively infect cho cells, had previously been shown to contain a host range gene, chohr, which confers on vaccinia virus the ability to replicate in cho cells (d. spehner, ...19957815529
restriction of vaccinia virus replication in cho cells occurs at the stage of viral intermediate protein synthesis.vaccina virus (vv) and cowpox virus (cpv) differ in their abilities to replicate in chinese hamster ovary (cho) cells because vv has a disrupted host range (hr) gene. to facilitate an examination of the molecular events associated with abortive infection of cho cells with vv, we constructed two sets of recombinant viruses that contain a viral early promoter regulating the cat gene encoding chloramphenicol acetyltransferase and viral intermediate or late promoters regulating the lacz gene encodin ...19957856109
a revised hindiii map and sequence analysis of a large 'left-hand' non-essential region of the rabbit poxvirus genome.sequence analysis of a 21.5 kb region of the left-hand non-essential region of rabbit poxvirus (rpv) was performed to analyze the structure and gene organization of this region. initial mapping and cloning studies revealed that the early published hindiii maps are incorrect in that the location of the terminal hindiii b and c fragments, as previously published, are reversed. the sequence of this region was compared to similar regions of several strains of vaccinia virus. the results indicate tha ...19938391191
recombinant protein synthesis in chinese hamster ovary cells using a vaccinia virus/bacteriophage t7 hybrid expression system.the vaccinia virus/bacteriophage t7 expression system was adapted to chinese hamster ovary (cho) cells. vaccinia virus undergoes abortive infection in cho cells, which is characterized by a sharp reduction in protein synthesis at the stage of viral intermediate gene expression. we determined that expression of a t7 promoter-regulated chloramphenicol acetyltransferase gene was at least 20 times more efficient in permissive bs-c-1 than in cho cells. the encephalomyocarditis virus 5'-untranslated r ...19968663285
isolation and characterization of a chinese hamster ovary mutant cell line with altered sensitivity to vaccinia virus killing.the chinese hamster ovary (cho) cell line is nonpermissive for vaccinia virus, and translation of viral intermediate genes was reported to be blocked (a. ramsey-ewing and b. moss, virology 206:984-993, 1995). however, cells are readily killed by vaccinia virus. a vaccinia virus-resistant cho mutant, vv5-4, was isolated by retroviral insertional mutagenesis. parental cho cells, upon infection with vaccinia virus, die within 2 to 3 days, whereas vv5-4 cells preferentially survive this cytotoxic ef ...19968676492
complementation of a vaccinia virus host-range k1l gene deletion by the nonhomologous cp77 gene.we investigated the host-range restriction of a vaccinia virus (vv) k1l deletion mutant in rabbit kidney rk13 cells and the ability of the nonhomologous cowpox virus cp77 gene to overcome this block. viral early mrnas were made by k1l- vv but early protein synthesis was arrested consistent with a translational block. replication of viral dna did not occur and neither intermediate nor late mrnas or proteins were detected. these results indicated that host-range restriction occurs earlier in rk13 ...19968806489
apoptosis induced by a postbinding step of vaccinia virus entry into chinese hamster ovary cells.unlike most cell types examined, nonpermissive chinese hamster ovary (cho) cells readily underwent apoptosis upon infection with vaccinia virus (vv). apoptosis was observed as early as 3 h postinfection with an electrophoretic assay of dna fragmentation and by 8 h using an in situ (tunel) assay. the cho hr gene from cowpox virus, which overcomes host range restriction of vv in cho cells, merely delayed the onset of apoptosis by approximately 3 h. intermediate and late viral protein synthesis wer ...19989501038
hepatitis c virus core protein does not inhibit apoptosis in human hepatoma cells.viral persistence is a major problem after infection with the hepatitis c virus. recently, it has been reported that hepatitis c virus core protein inhibits cis-platin induced apoptosis in human cervical carcinoma cells and apoptosis induced by overexpression of c-myc in chinese hamster ovary cells.199910583438
comparison and phylogenetic analysis of cowpox viruses isolated from cats and humans in fennoscandia.cowpox virus (cpxv), a member of the genus orthopoxvirus (opv), has reservoirs in small mammals and may cause disease in humans, felidae and other animals. in this study we compared cpxvs isolated from humans and cats in fennoscandia by restriction enzyme and dna sequence analysis. the hindiii restriction profiles clearly distinguished geographically distinct cpxv isolates, whereas only minor differences were found between the profiles of geographically linked isolates. the complete gene sequenc ...200919585075
generation and characterization of a cowpox virus mutant lacking host range factor cp77.cowpox virus (cpxv) host range factor cp77 was identified to be required for virus replication in chinese hamster ovary (cho) cells, but the underlying molecular mechanism by which cp77 modulates host range has remained unclear. therefore, a cpxvδcp77 deletion mutant was constructed by applying bacterial artificial chromosome (bac) technology. integrity of bac-derived viral dna was confirmed by whole genome sequencing. in vitro growth characteristics of cpxv wild type (wt), bac-derived vcpxv wt ...201222705200
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