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a one-step multiplex pcr to identify klebsiella pneumoniae, klebsiella variicola, and klebsiella quasipneumoniae in the clinical routine.klebsiella pneumoniae, klebsiella variicola and klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. we propose a multiplex pcr for blashv, blalen and blaokp and their flanking gene (deor). since this scheme focuses only on chromosomal genes, it will be feasible for klebsiella identification in the clinical routine.201728139276
genomic identification of nitrogen-fixing klebsiella variicola, k. pneumoniae and k. quasipneumoniae.it was difficult to differentiate klebsiella pneumoniae, k. quasipneumoniae and k. variicola by biochemical and phenotypic tests. genomics increase the resolution and credibility of taxonomy for closely-related species. here, we obtained the complete genome sequence of the k. variicola type strain dsm 15968(t) (=f2r9(t)). the genome of the type strain is a circular chromosome of 5,521,203 bp with 57.56% gc content. from 540 klebsiella strains whose genomes had been publicly available as at 3 mar ...201626471769
description of klebsiella quasipneumoniae sp. nov., isolated from human infections, with two subspecies, klebsiella quasipneumoniae subsp. quasipneumoniae subsp. nov. and klebsiella quasipneumoniae subsp. similipneumoniae subsp. nov., and demonstration that klebsiella singaporensis is a junior heterotypic synonym of klebsiella variicola.strains previously classified as members of klebsiella pneumoniae phylogroups kpi, kpii-a, kpii-b and kpiii were characterized by 16s rrna (rrs) gene sequencing, multilocus sequence analysis based on rpob, fusa, gapa, gyra and leus genes, average nucleotide identity and biochemical characteristics. phylogenetic analysis demonstrated that kpi and kpiii corresponded to k. pneumoniae and klebsiella variicola, respectively, whereas kpii-a and kpii-b formed two well-demarcated sequence clusters disti ...201424958762
whole-genome sequencing of human clinical klebsiella pneumoniae isolates reveals misidentification and misunderstandings of klebsiella pneumoniae, klebsiella variicola, and klebsiella quasipneumoniae.klebsiella pneumoniae is a major threat to public health, causing significant morbidity and mortality worldwide. the emergence of highly drug-resistant strains is particularly concerning. there has been a recognition and division of klebsiella pneumoniae into three distinct phylogenetic groups: klebsiella pneumoniae, klebsiella variicola, and klebsiella quasipneumoniae. k. variicola and k. quasipneumoniae have often been described as opportunistic pathogens that have less virulence in humans tha ...201728776045
draft genome sequence of ctx-m-type β-lactamase-producing klebsiella quasipneumoniae subsp. similipneumoniae isolated from a box turtle.klebsiella species has been regarded as a major pathogen that causes infections in humans and various animals. here, we report the draft genome sequence of a ctx-m-type β-lactamase-producing klebsiella quasipneumoniae subsp. similipneumoniae strain chkp0062 that was isolated from a yellow-margined box turtle.201729291945
klebsiella alba is a later heterotypic synonym of klebsiella quasipneumoniae subsp. similipneumoniae.the taxonomic position of klebsiella alba was re-examined. the reconstructed phylogenetic tree based on multilocus sequence analysis showed that klebsiella alba lmg 24441t (=kctc 12878t) was closely related to klebsiella quasipneumoniae subsp. similipneumoniae 07a044t, showing 99.5-100 % similarities for fusa, gapa, gyra, leus, pyrg, rpob, rpob2, atpd, gyrb and infb gene sequences and concatenated partial fusa, gapa, gyra, leus, pyrg, rpob2, atpd, gyrb and infb gene sequences. high sequence simi ...201627031705
colonization, infection, and the accessory genome of klebsiella pneumoniae.klebsiella pneumoniae is a gram-negative pathogen that has a large accessory genome of plasmids and chromosomal gene loci. this accessory genome divides k. pneumoniae strains into opportunistic, hypervirulent, and multidrug-resistant groups and separates k. pneumoniae from two closely related species, klebsiella variicola and klebsiella quasipneumoniae. some strains of k. pneumoniae act as opportunistic pathogens, infecting critically ill and immunocompromised patients. these k. pneumoniae are a ...201829404282
tigecycline susceptibility of klebsiella pneumoniae complex and escherichia coli isolates from companion animals: the prevalence of tigecycline-nonsusceptible k. pneumoniae complex, including internationally expanding human pathogenic lineages.transmission of tigecycline-nonsusceptible pathogenic enterobacteriaceae from companion animals to human should be a concern because tigecycline is a last-line drug for treating multidrug-resistant enterobacteriaceae in human medicine. however, tigecycline susceptibility of enterobacteriaceae isolated from companion animals has not been investigated. in this study, we investigated the tigecycline susceptibility of klebsiella pneumoniae complex and escherichia coli isolates from dogs and cats, an ...201729232167
characterization and antibiotic sensitivity profile of bacteria in orofacial abscesses of odontogenic origin.odontogenic infections range from peripheral abscess to superficial and deep infections leading to severe infections in head and neck region. this study was aimed to assess bacterial isolates responsible for orofacial infection of odontogenic origin and their drug susceptibility patterns so as to provide better perceptive for the management of odontogenic infections.201729038627
whole-genome sequences of two carbapenem-resistant klebsiella quasipneumoniae strains isolated from a tertiary hospital in johor, malaysia.we report the whole-genome sequences of two carbapenem-resistant clinical isolates of klebsiella quasipneumoniae subsp. similipneumoniae obtained from two different patients. both strains contained three different extended-spectrum β-lactamase genes and showed strikingly high pairwise average nucleotide identity of 99.99% despite being isolated 3 years apart from the same hospital.201728798179
draft genome of a hypervirulent klebsiella quasipneumoniae subsp. similipneumoniae with novel sequence type st2320 isolated from a chronic liver disease patient. 201728323229
genome sequence of klebsiella quasipneumoniae subsp. similipneumoniae mb373, an effective bioremediator.klebsiella quasipneumoniae subsp. similipneumoniae mb373 was isolated from effluent of the hattar industrial estate, haripur, pakistan. k. quasipneumoniae subsp. similipneumoniae has few cultivated/characterized members so far. whole-genome sequencing revealed its potential for metal and toxin resistance, which further elucidated various enzymatic processes for the degradation of xenobiotics, illuminating its bioremediation applications.201627688323
draft genome sequence of a hypermucoviscous extended-spectrum-β-lactamase-producing klebsiella quasipneumoniae subsp. similipneumoniae clinical isolate.a clinical isolate of extended-spectrum-β-lactamase-producing klebsiella quasipneumoniae subsp. similipneumoniae 06-219 with hypermucoviscosity phenotypes obtained from a urine culture of an adult patient was used for whole-genome sequencing. here, we report the draft genome sequences of this strain, consisting of 53 contigs with an ~5.6-mb genome size and an average g+c content of 57.36%. the annotation revealed 6,622 coding dna sequences and 77 trna genes.201627389261
complete genome sequence of klebsiella quasipneumoniae subsp. similipneumoniae strain atcc 700603.klebsiella quasipneumoniae subsp. similipneumoniae strain atcc 700603, formerly known as k. pneumoniae k6, is known for producing extended-spectrum β-lactamase (esbl) enzymes that can hydrolyze oxyimino-β-lactams, resulting in resistance to these drugs. we herein report the complete genome of strain atcc 700603 and show that the esbl genes are plasmid-encoded.201627231369
draft genome sequence of a multidrug-resistant klebsiella quasipneumoniae subsp. similipneumoniae isolate from a clinical source.we report here the draft genome sequence of a multidrug-resistant clinical isolate of klebsiella quasipneumoniae subsp. similipneumoniae, kp_z4175. this strain, isolated as part of a hospital infection-control screening program, is resistant to multiple β-lactam antibiotics, aminoglycosides, and trimethoprim-sulfamethoxazole.201627231362
liver abscess caused by infection with community-acquired klebsiella quasipneumoniae subsp. quasipneumoniae.we report a case of pyogenic liver abscess caused by community-acquired klebsiella quasipneumoniae subsp. quasipneumoniae. the infecting isolate had 2 prominent features of hypervirulent k. pneumoniae strains: the capsular polysaccharide synthesis region for k1 serotype and the integrative and conjugative element icekp1, which encodes the virulence factors yersiniabactin, salmochelin, and rmpa.201626890371
draft genome sequence of the first hypermucoviscous klebsiella quasipneumoniae subsp. quasipneumoniae isolate from a bloodstream infection.klebsiella quasipneumoniae is a recently described species, formerly identified as k. pneumoniae phylogroup kpii. information on pathogenic and virulence potential of this species are lacking. we sequenced the genome of a hypermucoviscous k. quasipneumoniae clinical isolate showing a virulence genes content (allabcdrs, kfuabc, and mrkabcdfhij) peculiar to hypervirulent k. pneumoniae strains.201526383657
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