| cdna, genomic sequence cloning and overexpression of glyceraldehyde-3-phosphate dehydrogenase gene (gapdh) from the giant panda. | gapdh (glyceraldehyde-3-phosphate dehydrogenase) is a key enzyme of the glycolytic pathway and it is related to the occurrence of some diseases. the cdna and the genomic sequence of gapdh were cloned successfully from the giant panda (ailuropoda melanoleuca) using the rt-pcr technology and touchdown-pcr, respectively. both sequences were analyzed preliminarily. the cdna of gapdh cloned from the giant panda is 1191 bp in size, contains an open reading frame of 1002 bp encoding 333 amino acids. th ... | 2010 | 20586183 |
| pharmacokinetics of ceftiofur crystalline-free acid in clinically healthy dogs (canis lupus familiaris). | economical, injectable antibiotics are beneficial when clinical manifestations of an animal model prevent the use of oral antibiotics. ceftiofur crystalline-free acid (ccfa) is an injectable, sustained-release form of ceftiofur, a third-generation cephalosporin that is labeled for use in swine, cattle, and horses. because ccfa is an economical, injectable antibiotic that could be of value for use in research dogs, the objective of this study was to determine the pharmacokinetic properties of ccf ... | 2016 | 27025816 |
| comparative proteomics of an extended spectrum β-lactamase producing escherichia coli strain from the iberian wolf. | the iberian wolf (canis lupus signatus) is an endangered species native to the iberian peninsula. due to their predatory and wild nature, these wolves serve as important indicators of environmental contamination by antimicrobial-resistant bacteria. β-lactam antibiotics like cefotaxime are the most commonly used antibacterial agents. bacterial resistance to these antibiotics occurs predominantly through enzymatic inactivation by extended-spectrum beta-lactamases. escherichia coli strain wa57, iso ... | 2014 | 24631823 |
| heterologous expression and functional characterization of matrix metalloproteinase-11 from canine mammary tumor. | matrix metalloproteinases (mmps) are reported to be involved in tumor growth, apoptosis, angiogenesis, invasion, and development of metastases. these are zinc containing metalloproteases, known for their role in extracellular matrix degradation. mmp-11 (stromelysin3) is reported to be highly expressed in breast cancer, therefore it may act as marker enzyme for breast cancer progression. the present work was carried out to produce recombinant canine (canis lupus familiaris) mmp-11 lacking the sig ... | 2013 | 23394368 |
| antimicrobial resistance in faecal enterococci and escherichia coli isolates recovered from iberian wolf. | the aim of this study was to report the antimicrobial resistance, the molecular mechanisms associated and the detection of virulence determinants within faecal enterococcus spp. and escherichia coli isolates of iberian wolf. enterococci (n = 227) and e. coli (n = 195) isolates were obtained from faecal samples of iberian wolf (canis lupus signatus). high rates of resistance were detected for tetracycline and erythromycin among the enterococci isolates, and most of resistant isolates harboured th ... | 2013 | 23336611 |
| cdna, genomic sequence cloning and overexpression of the ribosomal protein s13 gene in the giant panda (ailuropoda melanoleuca). | the cdna and the genomic sequence of ribosomal protein s13 (rps13) of the giant panda (ailuropoda melanoleuca) was cloned using reverse transcription-polymerase chain reaction (rt-pcr) and touchdown-pcr, respectively. these two sequences were sequenced and analyzed, and the cdna of the rps13 gene was overexpressed in escherichia coli bl21. we compared the nucleotide sequences of the coding region and the amino acid sequences with those of seven other mammalian species retrieved from genbank. the ... | 2011 | 21268784 |