| the mode of cauliflower mosaic virus propagation in the plant allows rapid amplification of viable mutant strains. | we inoculated the leaves of turnip plants (brassica campestris spp. rapa cv. just right) with two cauliflower mosaic viruses (camvs) with different small mutations in a dispensable region of the viral genome, and followed the spread of the virus infection through the plant. surprisingly, analysis of viral dna in single primary chlorotic lesions revealed the presence of both mutants. in contrast, the secondary chlorotic lesions and systemically infected leaves contained virus molecules of either ... | 1992 | 1607862 |
| genetic transformation of brassica campestris var. rapa protoplasts with an engineered cauliflower mosaic virus genome. | a hybrid cauliflower mosaic virus (camv) genome containing a selectable marker gene was constructed by replacing the gene vi coding region with the aminoglycoside (neomycin) phosphotransferase type ii [aph(3')ii] gene from tn5. this modified viral genome was tested for its infectivity both in planta and in a protoplast transformation system of brassica campestris var. rapa. stable, genetically transformed cell lines of b. campestris var. rapa were obtained after transformation. dna of the hybrid ... | 1986 | 24307380 |
| expression of mammalian metallothionein suppresses glucosinolate synthesis in brassica campestris. | transfection of brassica campestris leaves with cauliflower mosaic virus (camv) harboring a mammalian metallothionein (mt) cdna at the orfii position lowered the glucosinolate (gs) concentration to approximately one-half the level in leaves infected with wild-type camv. this suppression was independent of the plant's sulfate status, suggesting that the pathways for protein (mt) and gs biosynthesis were competing for s on an equal basis. the expression of mt may have lowered the endogenous levels ... | 1990 | 16667497 |
| genetic analysis of determinants of disease severity and virus concentration in cauliflower mosaic virus. | cauliflower mosaic virus (camv) strains cm1841 and w260 produced markedly different symptoms when inoculated onto turnips (brassica campestris l. 'just right'). the cm1841 strain induced a mild degree of stunting of infected plants while strain w260 caused moderate to severe stunting. although cm1841 was significantly milder than w260, it accumulated to a significantly higher concentration than w260 in systemically infected leaves. we constructed a series of hybrid viruses in order to map region ... | 1991 | 2014640 |
| detection of cleavage products from an in vivo transcribed cis hairpin ribozyme in turnips using the camv plant virus. | in order to examine ribozyme (rz) activity in vivo, we have adapted a virus to deliver rz to plants. dna fragments that code for both active and mutant cis-hairpin rz were cloned into the double-stranded dna plant virus, cauliflower mosaic virus (camv). these rz constructs successfully infected brassica campestris rapa (turnip). the plants that were infected with the active-rz construct showed, on average, a one-week delay in the appearance of viral symptoms, when compared to the mutant-rz contr ... | 1995 | 7622041 |
| the function of the flowering time gene agl20 is conserved in crucifers. | the mads box gene, agamous-like 20 (agl20), integrates environmental and endogenous flowering signals in arabidopsis thaliana. in order to determine if its role is conserved in other plants, we isolated agl20 orthologs from brassica campestris, cardamine flexuosa and draba nemorosa. the putative amino acid sequences of the orthologs were 94 to 97% identical. we analyzed the flowering phenotype and expression level of the agl20 ortholog in c. flexuosa, a long day plant that does not respond to ve ... | 2003 | 14503858 |
| agrobacterium-mediated transformation and regeneration of fertile transgenic plants of chinese cabbage (brassica campestris ssp. pekinensis cv. 'spring flavor'). | a procedure for the regeneration of fertile transgenic chinese cabbage (brassica campestris ssp. pekinensis cv. 'spring flavor') is presented in this report. the protocol is based on infection of cotyledon explants of 5-d-old seedlings with an agrobacterium tumefaciens strain lba4404 carrying a disarmed binary vector ptok/bks-1. the t-dna region of this binary vector contains the nopaline synthase/neomycin phosphotransferase ii (nptii) chimeric gene for kanamycin resistance and the cauliflower m ... | 1995 | 24194308 |
| point mutations in cauliflower mosaic virus gene vi confer host-specific symptom changes. | mutants of cauliflower mosaic virus (camv) strain d4 have been characterized with regard to host-specific phenotypes that resulted from specific changes in the viral dna sequence. both the mutant and the wild-type viruses infect a brassicaceous host, brassica campestris, systemically, giving indistinguishable symptoms. however, in the solanaceous host datura stramonium, which was systemically infectible by the wild-type virus, mutants induced necrotic local lesions at 21 degrees c and above, and ... | 2007 | 2134858 |
| characterization of a stamen-specific cdna encoding a novel plant defensin in chinese cabbage. | we isolated a stamen-specific cdna, bsd1 (brassica stamen specific plant defensin 1) that encodes a novel plant defensin peptide in chinese cabbage (brassica campestris l. ssp. pekinensis). plant defensins are antimicrobial peptides containing eight highly conserved cysteine residues linked by disulfide bridges. in bsd1, the eight cysteine residues and a glutamate residue at position 29 are conserved whereas other amino acid residues of the plant defensins consensus sequence are substituted. bsd ... | 2002 | 12139009 |