| viral metagenomics analysis of picobirnavirus-positive feces from children with sporadic diarrhea in china. | picobirnaviruses (pbvs) infect humans and a wide range of animals and may cause diarrhea. the aim of this study was to investigate pbv infection and its association with diarrhea. here, seven pbv rt-pcr-positive fecal samples from diarrheic children and four fecal samples from healthy children as controls were analyzed by viral metagenomics. the results indicated that all the seven diarrheic fecal samples contain high titers of pbv sequences, while three of the controls were negative, and one ha ... | 2016 | 26695771 |
| a divergent clade of circular single-stranded dna viruses from pig feces. | using metagenomics and molecular cloning methods, we characterized five novel small, circular viral genomes from pig feces that are distantly related to chimpanzee and porcine stool-associated circular viruses, (chiscv and poscv1). phylogenetic analysis placed these viruses into a highly divergent clade of this rapidly growing new viral family. this new clade of viruses, provisionally named porcine stool-associated circular virus 2 and 3 (poscv2 and poscv3), encodes a stem-loop structure (presum ... | 2013 | 23612924 |
| identification and genetic characterization of a novel circular single-stranded dna virus in a human upper respiratory tract sample. | metagenomic analysis through high-throughput sequencing is a tool for detecting both known and novel viruses. using this technique, a novel circular single-stranded dna (ssdna) virus genome was discovered in respiratory secretions from a febrile traveler. the virus, named human respiratory-associated pscv-5-like virus (hraplv), has a genome comprising 3,018 bases, with two major putative orfs inversely encoding capsid (cap) and replicase (rep) protein and separated by two intergenic regions. one ... | 2017 | 28707271 |
| identification of a novel single-stranded circular dna virus in pig feces. | porcine stool-associated circular virus 5 (poscv5) was detected in the feces of a pig with diarrhea. the complete 3,062-nucleotide genome contains two bidirectionally transcribed open reading frames (orfs). phylogenetic analysis of the deduced replication initiator protein (rep) places poscv5 alone on a deep branch among the small circular rep-encoding single-stranded dna viruses. | 2014 | 24786952 |