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comparative efficacy of house dust mite extermination products.the acaricidal efficacy of nine marketed products, i.e. acardust, acarosan (foam and powder), actelic 50, artilin 3a (spirit and water base), liquid nitrogen, paragerm ak, and tymasil, and of intensive vacuum-cleaning have been compared on four different test surfaces: mattress, tufted carpet, gypsum board and rough wooden board, all covered with artificial house dust. they were inoculated with the house dust mite, dermatophagoides pteronyssinus or the house-dust fungus aspergillus repens for ev ...19921393759
fungi of the house dust in riyadh, saudi arabia.30 species belonging to 18 genera were isolated from floor dust of 30 homes in riyadh. out of them 16 species and 10 genera were isolated from dust of air conditioners of the same homes. the most common genera in floor dust were aspergillus, penicillium and cladosporium. aspergillus repens, a. amstelodami, a. versicolor, a. fumigatus, penicillium purpurogenum, p. crustosum, cladosporium cladosporoides and c. herbarum were frequently isolated. the most abundant genera in air conditioner dust were ...19902330766
odor volatiles associated with microflora in damp ventilated and non-ventilated bin-stored bulk wheat.western hard red spring wheat, stored at 20 and 25% moisture contents for 10 months during 1985-86, was monitored for biotic and abiotic variables in 10 unheated bins in winnipeg, manitoba. the major odor volatiles identified were 3-methyl-1-butanol, 3-octanone and 1-octen-3-ol. the production of these volatiles was associated and correlated with microfloral infection. ventilation, used for cooling and drying of grain, disrupted microfloral growth patterns and production of volatiles. the highes ...19892641886
fungal volatiles associated with moldy grain in ventilated and non-ventilated bin-stored wheat.the fungal odor compounds 3-methyl-1-butanol, 1-octen-3-ol and 3-octanone were monitored in nine experimental bins in winnipeg, manitoba containing a hard red spring wheat during the autumn, winter and summer seasons of 1984-85. quality changes were associated with seed-borne microflora and moisture content in both ventilated and non-ventilated bins containing wheat of 15.6 and 18.2% initial moisture content. all three odor compounds occurred in considerably greater amounts in bulk wheat in non- ...19883352722
purification and properties of extracellular polysaccharide (eps) antigens produced by different mould species.extracellular polysaccharide (eps) antigens produced by different mould species were purified and partially characterized. purification included (nh4)2so4 treatment, sepharose cl-4b column chromatography and con a-sepharose chromatography. the eps of penicillium digitatum, mucor racemosus and cladosporium cladosporioides showed high antigenic capacities. immunologically the eps were partially genus-specific, but cross-reactivity was observed. the eps antigens produced by species of penicillium, ...19873571038
fungal growth and the presence of sterigmatocystin in hard cheese.molds isolated from visibly molded cheeses in shops, households, and warehouses have been identified. mold flora of cheeses in shops and households consisted mainly of penicillium verrucosum var. cyclopium. on cheeses ripening in warehouses, penicillium verrucosum var. cyclopium, aspergillus versicolor, aspergillus repens, and enicillium verrucosum var. verrucosum were the dominant mold species. cheese ripening in warehouses and molded with a. versicolor were examined for sterigmatocystin. nine ...19807380782
fungicidal effect of 15 disinfectants against 25 fungal contaminants commonly found in bread and cheese manufacturing.resistance of 19 mold and 6 yeast species to 15 commercial disinfectants was investigated by using a suspension method in which the fungicidal effect and germination time were determined at 20 degrees c. disinfectants containing 0.5% dodecyldiethylentriaminacetic acid, 10 g of chloramine-t per 1, 2.0% formaldehyde, 0.1% potassium hydroxide, 3.0% hydrogen peroxide, or 0.3% peracetic acid were ineffective as fungicides. the fungicidal effect of quaternary ammonium compounds and chlorine compounds ...199610463445
fungicidal effect of 15 disinfectants against 25 fungal contaminants commonly found in bread and cheese manufacturing.resistance of 19 mold and 6 yeast species to 15 commercial disinfectants was investigated by using a suspension method in which the fungicidal effect and germination time were determined at 20 degrees c. disinfectants containing 0.5% dodecyldiethylentriaminacetic acid, 10 g of chloramine-t per 1, 2.0% formaldehyde, 0.1% potassium hydroxide, 3.0% hydrogen peroxide, or 0.3% peracetic acid were ineffective as fungicides. the fungicidal effect of quaternary ammonium compounds and chlorine compounds ...199610463445
purification and characterization of novel antifungal compounds from the sourdough lactobacillus plantarum strain 21b.sourdough lactic acid bacteria were selected for antifungal activity by a conidial germination assay. the 10-fold-concentrated culture filtrate of lactobacillus plantarum 21b grown in wheat flour hydrolysate almost completely inhibited eurotium repens ibt18000, eurotium rubrum ftdc3228, penicillium corylophilum ibt6978, penicillium roqueforti ibt18687, penicillium expansum idm/fs2, endomyces fibuliger ibt605 and idm3812, aspergillus niger ftdc3227 and idm1, aspergillus flavus ftdc3226, monilia s ...200010966432
purification and characterization of novel antifungal compounds from the sourdough lactobacillus plantarum strain 21b.sourdough lactic acid bacteria were selected for antifungal activity by a conidial germination assay. the 10-fold-concentrated culture filtrate of lactobacillus plantarum 21b grown in wheat flour hydrolysate almost completely inhibited eurotium repens ibt18000, eurotium rubrum ftdc3228, penicillium corylophilum ibt6978, penicillium roqueforti ibt18687, penicillium expansum idm/fs2, endomyces fibuliger ibt605 and idm3812, aspergillus niger ftdc3227 and idm1, aspergillus flavus ftdc3226, monilia s ...200010966432
modified atmosphere packaging for prevention of mold spoilage of bakery products with different ph and water activity levels.a sponge cake analog was used to study the influence of ph, water activity (aw), and carbon dioxide (co2) levels on the growth of seven fungal species commonly causing bakery product spoilage (eurotium amstelodami, eurotium herbariorum, eurotium repens, eurotium rubrum, aspergillus niger, aspergillus flavus, and penicillium corylophilum). a full factorial design was used. water activity, co2, and their interaction were the main factors significantly affecting fungal growth. water activity at lev ...200314572225
modified atmosphere packaging for prevention of mold spoilage of bakery products with different ph and water activity levels.a sponge cake analog was used to study the influence of ph, water activity (aw), and carbon dioxide (co2) levels on the growth of seven fungal species commonly causing bakery product spoilage (eurotium amstelodami, eurotium herbariorum, eurotium repens, eurotium rubrum, aspergillus niger, aspergillus flavus, and penicillium corylophilum). a full factorial design was used. water activity, co2, and their interaction were the main factors significantly affecting fungal growth. water activity at lev ...200314572225
early detection of fungal growth in bakery products by use of an electronic nose based on mass spectrometry.this paper presents the design, optimization, and evaluation of a mass spectrometry-based electronic nose (ms e-nose) for early detection of unwanted fungal growth in bakery products. seven fungal species (aspergillus flavus, aspergillus niger, eurotium amstelodami, eurotium herbariorum, eurotium rubrum, eurotium repens, and penicillium corylophillum) were isolated from bakery products and used for the study. two sampling headspace techniques were tested: static headspace (sh) and solid-phase mi ...200415453668
early detection of fungal growth in bakery products by use of an electronic nose based on mass spectrometry.this paper presents the design, optimization, and evaluation of a mass spectrometry-based electronic nose (ms e-nose) for early detection of unwanted fungal growth in bakery products. seven fungal species (aspergillus flavus, aspergillus niger, eurotium amstelodami, eurotium herbariorum, eurotium rubrum, eurotium repens, and penicillium corylophillum) were isolated from bakery products and used for the study. two sampling headspace techniques were tested: static headspace (sh) and solid-phase mi ...200415453668
mites and fungi in heavily infested stores in the czech republic.toxigenic and allergen-producing fungi represent a serious hazard to human food and animal feed safety. ninety-four fungal species were isolated from mite-infested samples of seeds taken from czech seed stores. fungi were isolated from the surface of four kinds of seeds (wheat, poppy, lettuce, and mustard) and from the gut and external surface of five species of mites (i.e., acarus siro l., 1758, caloglyphus rhizoglyphoides (zachvatkin, 1973), lepidoglyphus destructor (schrank, 1781), tyrophagus ...200415666776
mites and fungi in heavily infested stores in the czech republic.toxigenic and allergen-producing fungi represent a serious hazard to human food and animal feed safety. ninety-four fungal species were isolated from mite-infested samples of seeds taken from czech seed stores. fungi were isolated from the surface of four kinds of seeds (wheat, poppy, lettuce, and mustard) and from the gut and external surface of five species of mites (i.e., acarus siro l., 1758, caloglyphus rhizoglyphoides (zachvatkin, 1973), lepidoglyphus destructor (schrank, 1781), tyrophagus ...200415666776
evaluation of mycological contamination of dental unit waterlines.the quality of dental unit water is of great importance since patients and dental staff are regularly exposed to water from aerosols generated during work. the main purpose of this investigation was mycological evaluation of dental unit waterlines (duwl). the author determined the number and species of fungi present in the water from a unit reservoir which is the source of water for a dental unit, in the water flowing from a high-speed handpiece of a unit, and in the swab sample collected from t ...200516028882
fungi isolated from flue-cured tobacco at time of sale and after storage.the fungi isolated from 100 samples of flue-cured tobacco from 12 markets in 2 tobacco belts comprised 11 genera, including 10 species of aspergillus. the mean percentage per sample isolated from 62 samples of tobacco from middle belt markets was alternaria, 40.6%; aspergillus niger, 47.8%; aspergillus repens, 38.0%; and penicillium, 25.8%. the mean percentage per sample isolated from 38 samples of tobacco from old belt markets was alternaria, 74.0%; penicillium, 52.5%; aspergillus repens, 38.0% ...196916349841
growth of aspergillus repens in flue-cured tobacco.in laboratory tests, flue-cured tobacco inoculated with aspergillus repens was stored at 75, 80, 85, 87, and 95% relative humidity at 20 and 30 c. samples were taken weekly for 4 weeks and evaluated for mold growth (colony count) and moisture content (mc). the weekly rate of fungus increase was slower at 20 c than at 30 c. tobacco at 20 c with mc between 25 to 30% supported a slight to moderate increase in a. repens after 3 weeks of storage. however, tobacco at the same mc stored at 30 c was sub ...197116349905
development of flexible antimicrobial films using essential oils as active agents.the antimicrobial activity in the vapor-phase of laboratory-made flexible films of polypropylene (pp) and polyethylene/ethylene vinyl alcohol copolymer (pe/evoh) incorporating essential oil of cinnamon ( cinnamomum zeylanicum), oregano ( origanum vulgare), clove ( syzygium aromaticum), or cinnamon fortified with cinnamaldehyde was evaluated against a wide range of microorganisms: the gram-negative bacteria escherichia coli, yersinia enterocolitica, pseudomonas aeruginosa, and salmonella cholerae ...200717880148
development of flexible antimicrobial films using essential oils as active agents.the antimicrobial activity in the vapor-phase of laboratory-made flexible films of polypropylene (pp) and polyethylene/ethylene vinyl alcohol copolymer (pe/evoh) incorporating essential oil of cinnamon ( cinnamomum zeylanicum), oregano ( origanum vulgare), clove ( syzygium aromaticum), or cinnamon fortified with cinnamaldehyde was evaluated against a wide range of microorganisms: the gram-negative bacteria escherichia coli, yersinia enterocolitica, pseudomonas aeruginosa, and salmonella cholerae ...200717880148
fungal growth inhibitory properties of new phytosphingolipid analogues.to study the growth inhibitory properties of a series of phytosphingosine (phs) and phytoceramide (phc) analogues.200818005346
effect of mixed antimicrobial agents and flavors in active packaging films.active packaging is an emerging food technology to improve the quality and safety of food products. many works have been developed to study the antimicrobial activity of essential oils. essential oils have been traditionally used as flavorings in food, so they have an important odor impact but they have as well antimicrobial properties that could be used to protect the food. recent developments in antimicrobial active packaging showed the efficiency of essential oils versus bread and bakery prod ...200919711918
effect of mixed antimicrobial agents and flavors in active packaging films.active packaging is an emerging food technology to improve the quality and safety of food products. many works have been developed to study the antimicrobial activity of essential oils. essential oils have been traditionally used as flavorings in food, so they have an important odor impact but they have as well antimicrobial properties that could be used to protect the food. recent developments in antimicrobial active packaging showed the efficiency of essential oils versus bread and bakery prod ...200919711918
benzyl derivatives with in vitro binding affinity for human opioid and cannabinoid receptors from the fungus eurotium repens.bioassay-guided fractionation of the fungus eurotium repens resulted in the isolation of two new benzyl derivatives, (e)-2-(hept-1-enyl)-3-(hydroxymethyl)-5-(3-methylbut-2-enyl)benzene-1,4-diol (1) and (e)-4-(hept-1-enyl)-7-(3-methylbut-2-enyl)-2,3-dihydrobenzofuran-2,5-diol (2), along with seven known compounds (3-9) including five benzaldehyde compounds, flavoglaucin (3), tetrahydroauroglaucin (4), dihydroauroglaucin (5), auroglaucin (6), and 2-(2',3-epoxy-1',3'- heptadienyl)-6-hydroxy-5-(3-me ...201121667972
benzyl derivatives with in vitro binding affinity for human opioid and cannabinoid receptors from the fungus eurotium repens.bioassay-guided fractionation of the fungus eurotium repens resulted in the isolation of two new benzyl derivatives, (e)-2-(hept-1-enyl)-3-(hydroxymethyl)-5-(3-methylbut-2-enyl)benzene-1,4-diol (1) and (e)-4-(hept-1-enyl)-7-(3-methylbut-2-enyl)-2,3-dihydrobenzofuran-2,5-diol (2), along with seven known compounds (3-9) including five benzaldehyde compounds, flavoglaucin (3), tetrahydroauroglaucin (4), dihydroauroglaucin (5), auroglaucin (6), and 2-(2',3-epoxy-1',3'- heptadienyl)-6-hydroxy-5-(3-me ...201121667972
antimicrobial and antiprotozoal activities of secondary metabolites from the fungus eurotium repens.in this study, we examined in vitro antibacterial, antifungal, antimalarial, and antileishmanial activities of secondary metabolites (1-8) isolated from the fungus eurotium repens. all compounds showed mild to moderate antibacterial or antifungal or both activities except 7. the activity of compound 6 was the best of the group tested. the in vitro antimalarial evaluation of these compounds revealed that compounds 1-3, 5, and 6 showed antimalarial activities against both chloroquine-sensitive and ...201123024574
antimicrobial and antiprotozoal activities of secondary metabolites from the fungus eurotium repens.in this study, we examined in vitro antibacterial, antifungal, antimalarial, and antileishmanial activities of secondary metabolites (1-8) isolated from the fungus eurotium repens. all compounds showed mild to moderate antibacterial or antifungal or both activities except 7. the activity of compound 6 was the best of the group tested. the in vitro antimalarial evaluation of these compounds revealed that compounds 1-3, 5, and 6 showed antimalarial activities against both chloroquine-sensitive and ...201123024574
microbial diversity in the gut of cashew stem girdler, analeptes trifasciata fabricius (coleoptera: cerambycidae), in ibadan, nigeria.the cashew stem girdler, analeptes trifasciata, is a major insect pest of cashew in nigeria causing economic damage in cashew plantations even at low density. in this study, newly emerged adults of a. trifasciata reared from field-infested cashew stems were collected from the rearing cages, sexed, and dissected to reveal the internal structures of the insects. the gut was excised and separated into the foregut, midgut, and hindgut. the dissected gut compartments were blotted dry by sandwiching i ...201627147898
on the problem of nonsense correlations in allergological tests after routine extraction.the influence of extraction procedures and culturing methods of material used for the preparation of allergenic extracts on correlation patterns found in allergological testing (skin test and rast) was investigated. in our laboratory a short extraction procedure performed at o degrees c was used for aspergillus repens. a. penicilloides, wallemia sebi, their rearing media and non-inoculated medium. for the commercially available extracts from house dust, house-dust mite, pollen of dactylus glomer ...19817027777
fungi isolated from flue-cured tobacco sold in southeast united states, 1968-1970.flue-cured tobacco leaves, from low- and middle-stalk positions, offered for sale in each of two markets, within each of five tobacco types, were evaluated for moisture content (mc) and filamentous fungi during august through october in 1968, 1969, and 1970. alternaria alternata, penicillium cyclopium, aspergillus niger, aspergillus repens, and aspergillus flavus were most frequently isolated from cultured tissue. other filamentous fungi that grew from the tissue included species from four gener ...19724627970
modeling growth of three bakery product spoilage molds as a function of water activity, temperature and ph.the objective of this study was to quantify the effect of water activity, ph and storage temperature on the growth of eurotium repens, aspergillus niger and penicillium corylophilum, isolated from spoiled bakery products. moreover, the behaviors of these three mold species were compared to assess whether a general modeling framework may be set and re-used in future research on bakery spoilage molds. the mold growth was modeled by building two distinct gamma-type secondary models: one on the lag ...201425016208
fungal diversity of rice straw for meju fermentation.rice straw is closely associated with meju fermentation and it is generally known that the rice straw provides meju with many kinds of microorganisms. in order to elucidate the origin of meju fungi, the fungal diversity of rice straw was examined. rice straw was collected from 12 jang factories where meju are produced, and were incubated under nine different conditions by altering the media (mea, drbc, and dg18), and temperature (15°c, 25°c, and 35°c). in total, 937 strains were isolated and ide ...201324043125
antimicrobial constituents from three endophytic fungi.to evaluate the antimicrobial potential of extracts of the endophytic fungi plectophomella sp., physalospora sp., and crataegus monogyna (c. monogyna) and study the tentative identification of their active constituents.201425312126
chlorine inactivation of fungal spores on cereal grains.although 0.4% chlorine for 2 min has been recommended for surface disinfection of food samples before direct plating for fungal enumeration, this procedure may not be adequate for highly contaminated products. the effectiveness of a range of chlorine solutions was investigated using barley samples artificially contaminated with four different concentrations of aspergillus flavus. a. niger, a. ochraceus, eurotium repens, penicillium brevicompactum p. chrysogenum and cladosporium cladosporioides. ...19979105923
heterologous expression and characterization of the aspergillus aspartic protease involved in the hydrolysis and decolorization of red-pigmented proteins.aspergillus repens strain mk82 produces an aspartic protease (pepa_mk82) that efficiently decolorizes red-pigmented proteins during dried bonito fermentation. however, further expansion of the industrial applications of pepa_mk82 requires the high-level production and efficient preparation of the recombinant enzyme.201626919469
chlorine inactivation of fungal spores on cereal grains.although 0.4% chlorine for 2 min has been recommended for surface disinfection of food samples before direct plating for fungal enumeration, this procedure may not be adequate for highly contaminated products. the effectiveness of a range of chlorine solutions was investigated using barley samples artificially contaminated with four different concentrations of aspergillus flavus. a. niger, a. ochraceus, eurotium repens, penicillium brevicompactum p. chrysogenum and cladosporium cladosporioides. ...19979105923
sodium chloride stress induced morphological and ultrastructural changes in aspergillus repens.halotolerant fungus, a. repens, showed a considerable difference in its growth rate, morphology, ultrastructural and molecular composition under nacl stress as compared to control i.e. non-stressed condition. light microscopic observations revealed significant differences in their mycelial thickness, their branching and septa. transmission electron microscopic observations of both the conditions depicted significant differences in the qualitative and quantitative changes in mitochondria. frequen ...19938406598
aspartic protease from aspergillus (eurotium) repens strain mk82 is involved in the hydrolysis and decolourisation of dried bonito (katsuobushi).katsuobushi is a dried, smoked and fermented bonito used in japanese cuisine. during the fermentation process with several aspergillus species, the colour of katsuobushi gradually changes from a dark reddish-brown derived from haem proteins to pale pink. the change in colour gives katsuobushi a higher ranking and price. this study aimed to elucidate the mechanism of decolourisation of katsuobushi.201323044751
selection of sourdough lactobacilli with antifungal activity for use as biopreservatives in bakery products.two hundred and sixteen lab cultures from sourdoughs and dough for bread and panettone production were screened for in vitro antifungal properties against three indicator cultures ascribed to aspergillus japonicus , eurotium repens , and penicillium roseopurpureum , isolated from bakery environment and moldy panettone. nineteen preselected isolates were subjected to minimum inhibitory concentration determination against the indicator cultures. sourdoughs prepared with the two most promising stra ...201222794119
analyses of fungal community by illumina miseq platforms and characterization of eurotium species on liupao tea, a distinctive post-fermented tea from china.liupao tea is a distinctive chinese dark tea obtained by indigenous tea fermentation facilitated by the symbiotic association of bacteria and fungi. the composition of fungal community in 4 liupao tea samples stored for several years under natural microbial fermentation was evaluated by miseq sequencing. taxonomic analysis revealed 3 phyla, 6 families, 8 genera. the genera eurotium and aspergillus were dominant fungi in almost all the samples. a total of 85 strains found in 41 other tea samples ...201728784527
mycoflora of soybeans used for meju fermentation.diverse fungi are present in korean traditional meju and they are known to play an important role in fermented soybean products. to determine the origin of the fungi in meju, we examined the mycoflora of soybeans from 10 traditional meju factories. the samples were untreated or treated with sodium hypochlorite, and placed on malt extract agar (mea), dichloran 18% glycerol agar (dg18), and dichloran rose bengal chloramphenicol agar (drbc) medium. a total of 794 fungal strains were isolated and th ...201323874133
maxillary sinusitis from microascus cinereus and aspergillus repens.microascus was associated with aspergillus repens in a left maxillary sinus. tissue contained septale filaments of two types, conidia, ostiolate perithecia containing ascospores corresponding to microascus cinereus which was identified by culture. the abundance of sexual fructifications in the tissue indicates that pathogenicity is due to microascus cinereus.19892747787
aspergillus associated with meju, a fermented soybean starting material for traditional soy sauce and soybean paste in korea.aspergillus is an important fungal genus used for the fermentation of asian foods; this genus is referred to as koji mold in japan and china. a. oryzae, a. sojae, and a. tamari are used in the production of miso and shoyu in japan, but a comprehensive taxonomic study of aspergillus isolated from meju, a fermented soybean starting material for traditional soy sauce and soybean paste in korea, has not been conducted. in this study, various aspergillus species were isolated during a study of the my ...201526539037
mycological contamination in dental unit waterlines in istanbul, turkey.studies on dental units (dus) are conducted either for the prevention or the reduction of the density of bacterial contamination in dental unit waterlines (duwls). however, the existence of fungi in the these systems requires more attention. during dental treatment, direct contact with water contaminated with fungi such as candida, aspergillus, or inhalation of aerosols from high-speed drill may cause various respiratory infections, such as asthma, allergies, and wounds on mucose membranes, espe ...201324516467
quantifying effect of lactic, acetic, and propionic acids on growth of molds isolated from spoiled bakery products.the combined effect of undissociated lactic acid (0 to 180 mmol/liter), acetic acid (0 to 60 mmol/liter), and propionic acid (0 to 12 mmol/liter) on growth of the molds aspergillus niger, penicillium corylophilum, and eurotium repens was quantified at ph 3.8 and 25°c on malt extract agar acid medium. the impact of these acids on lag time for growth (λ) was quantified through a gamma model based on the mic. the impact of these acids on radial growth rate (μ) was analyzed statistically through pol ...201526319723
optical analysis of reduction products of 2-methylcyclohexanone by aspergillus repens ma0197.the products of reduction of 2-methylcyclohexanone by aspergillus repens ma0197 were analyzed quantitatively by gc after conversion to corresponding diastereoisomeric ( -)-menthyl carbonate derivatives. although the contents varied considerably with time, the predominant production of s-alcohol was observed, that is, prelog's rule was obeyed.199627299552
polyol concentrations in aspergillus repens grown under salt stress.na(+), k(+) and the ratio of na(+)/k(+) were higher in cells of the halotolerant aspergillus repens grown with 2 m nacl than without nacl. the osmolytes, proline, glycerol, betaine and glutamate, did not affect the na(+)/k(+) ratio, nor the polyol content of cells under any conditions. the concentrations of polyols, consisting of glycerol, arabitol, erythritol and mannitol, changed markedly during growth, indicating that they have a crucial role in osmotic adaptation.199324420204
relations of enzymes inaspergillus repens grown under sodium chloride stress.aspergillus repens, a salt-pan isolate, was halotolerant. when grown for 72 h (log phase) and 144 h (beginning of stationary phase) in a medium containing 2m sodium chloride, the activities of invertase, malate dehydrogenase (mdh), glucose-6-phosphate dehydrogenase (g6pdh), and glutamate dehydrogenase (gdh) were found to have increased. control cultures grown in a medium devoid of 2m nacl failed to show such changes. the activities of mdh, g6pdh, and gdh increased with rising concentrations of n ...199323835748
quantifying the effect of water activity and storage temperature on single spore lag times of three moulds isolated from spoiled bakery products.the inhibitory effect of water activity (aw) and storage temperature on single spore lag times of aspergillus niger, eurotium repens (aspergillus pseudoglaucus) and penicillium corylophilum strains isolated from spoiled bakery products, was quantified. a full factorial design was set up for each strain. data were collected at levels of aw varying from 0.80 to 0.98 and temperature from 15 to 35°c. experiments were performed on malt agar, at ph5.5. when growth was observed, ca 20 individual growth ...201727325576
identification and quantification of antifungal compounds produced by lactic acid bacteria and propionibacteria.fungal growth in bakery products represents the most frequent cause of spoilage and leads to economic losses for industrials and consumers. bacteria, such as lactic acid bacteria and propionibacteria, are commonly known to play an active role in preservation of fermented food, producing a large range of antifungal metabolites. in a previous study (le lay et al., 2016), an extensive screening performed both in vitro and in situ allowed for the selection of bacteria exhibiting an antifungal activi ...201627350657
[effect of water substrate activity and relative air humidity on penicillium chrysogenum thom, aspergillus repens (corda) sacc., trichoderma viride pers., isolated from dwelling premises]. 201121861374
stimulating effect of sorbitol and xylitol on germination and growth of some xerophilic fungi.sorbitol and xylitol are polyols often used in foods as naturally occurring sugar substitutes. they provide sweet taste and reduced calories in products of intermediate moisture. this type of food is susceptible to spoilage by xerophilic molds which affect shelf life of foods and produce significant losses. the aim of the present work was to study the effect of glycerol, sorbitol and xylitol on the germination and growth of four xerophilic fungi at different temperatures and water activity level ...201121925029
stimulating effect of sorbitol and xylitol on germination and growth of some xerophilic fungi.sorbitol and xylitol are polyols often used in foods as naturally occurring sugar substitutes. they provide sweet taste and reduced calories in products of intermediate moisture. this type of food is susceptible to spoilage by xerophilic molds which affect shelf life of foods and produce significant losses. the aim of the present work was to study the effect of glycerol, sorbitol and xylitol on the germination and growth of four xerophilic fungi at different temperatures and water activity level ...201121925029
absolute real-time measurement of particle size distribution with the flying light-scattering indicatrix method.the flying light-scattering indicatrix (flsi, angular dependency of the intensity of light scattered by a moving individual particle) method, based on a scanning flow cytometer (sfc) that permits measurement of individual particle characteristics from light-scattering data, has been used for the determination of size distribution of the following particles: polystyrene latex, milk fat, and spores (penicillium levitum, aspergillus pseudoglaucus). the optical system of the spc and empirical equati ...199621102713
mycoflora and mycotoxin production in oilseed cakes during farm storage.agricultural activities involve the use of oilseed cakes as a source of proteins for livestock. because the storage of oilseed cakes could induce the development of molds and the production of mycotoxins, a survey was conducted during the 5 months of farm storage. mycoflora was studied by microscopic examinations, and the presence of aspergillus fumigatus was confirmed by polymerase chain reaction. a multimycotoxin method was developed to quantify seven mycotoxins (aflatoxin b(1), alternariol, f ...200919183000
[collagenolytic activity in several species of deuteromycetes under various storage conditions].the ability of deuteromycetes of the genera penicillium, aspergillus, and botrytis to retain collagenolytic activity was studied after both 2 and 10 years of storage on a czapek medium under a layer of mineral oil at 4 degrees c, as well as in silica gel granules at 20 and -60 degrees c. the enzymatic activity of several species, including botrytis terrestris, penicillium janthinellum, penicillium chrysogenum, and penicillium citrinum, was retained under both conditions of storage. aspergillus r ...200617022462
toxic micromycetes in grain raw material during its processing.in 2003-2005 micromycetes were isolated and identified from wheat, barley, rye, buckwheat grain brought into mills or from processing enterprises. contamination of the produced flour with micromycete propagules (cfu g(-1)), changes in micromycete diversity and abundance in the course of flour storage, preparation and baking of bread, production of groats or other food products and fodder were determined. most attention was given to widely distributed micromycetes, known producers of toxins: alte ...200616841886
toxic micromycetes in grain raw material during its processing.in 2003-2005 micromycetes were isolated and identified from wheat, barley, rye, buckwheat grain brought into mills or from processing enterprises. contamination of the produced flour with micromycete propagules (cfu g(-1)), changes in micromycete diversity and abundance in the course of flour storage, preparation and baking of bread, production of groats or other food products and fodder were determined. most attention was given to widely distributed micromycetes, known producers of toxins: alte ...200616841886
analysis of fungi detected in human cadavers.we examined and identified fungi flora found growing on two human cadavers. the main isolate colonized on the surfaces of the skin and bones was morphologically identified as eurotium repens. the genus eurotium usually inhabits soil and exhibits osmophilic properties. because the cadavers contained abundant organic materials, eurotium species formed ascomata and conidia. to our knowledge, this is the first study to describe in detail the species of fungus obtained from human cadavers. because th ...200616516528
analysis of fungi detected in human cadavers.we examined and identified fungi flora found growing on two human cadavers. the main isolate colonized on the surfaces of the skin and bones was morphologically identified as eurotium repens. the genus eurotium usually inhabits soil and exhibits osmophilic properties. because the cadavers contained abundant organic materials, eurotium species formed ascomata and conidia. to our knowledge, this is the first study to describe in detail the species of fungus obtained from human cadavers. because th ...200616516528
influence of activation and germination on high pressure inactivation of ascospores of the mould eurotium repens.we investigated heat activation and germination of eurotium repens ascospores to follow high pressure inactivation. activation energy and entropy values strengthen the idea of protein denaturation as the underlying mechanism of heat activation. preceding activation, germination or a combination of both affected high pressure inactivation in different ways. activation followed immediately by high pressure treatment led to the most efficient improvement in inactivation. however, a pause after acti ...200211867285
influence of activation and germination on high pressure inactivation of ascospores of the mould eurotium repens.we investigated heat activation and germination of eurotium repens ascospores to follow high pressure inactivation. activation energy and entropy values strengthen the idea of protein denaturation as the underlying mechanism of heat activation. preceding activation, germination or a combination of both affected high pressure inactivation in different ways. activation followed immediately by high pressure treatment led to the most efficient improvement in inactivation. however, a pause after acti ...200211867285
detection and identification of xerophilic fungi in belgian chocolate confectionery factories.chocolate confectionery fillings are generally regarded as microbiologically stable. the stability of these fillings is largely due to the general practice of adding either alcohol or preservatives. consumer demands are now stimulating producers to move away from adding alcohol or other preservatives to their confectionery fillings and instead to search for innovative formulations. such changes in composition can influence the shelf life of the product and may lead to spoilage by xerophilic fung ...201525475302
ecological relationships between xerophilic fungi and house-dust mites (acarida: pyroglyphidae).at. 75 and 80% relative humidity (rh), on a wheat germ flake medium, aspergillus penicilloides grew abundantly and suppressed the population growth of dermatophagoides pteronyssiunus. at 71% rh, a. penicilloides grew moderately and was only antagonistic to d. pteronyssinus when the fungus was previously incubated on the medium.on a human dander medium and on mattress dust, a. penicilloides grew moderately at 71% and 75% rh and stimulated the development of d. pteronyssinus populations. also a mo ...197828309597
cytotoxic and genotoxic potencies of single and combined spore extracts of airborne ota-producing and ota-non-producing aspergilli in human lung a549 cells.aspergillus sclerotiorum (as) is a well-known producer of ochratoxin a (ota) while aspergillus pseudoglaucus (ap) produces a wide range of extrolites with poorly investigated toxicity. these species are frequently co-occur in grain mill aeromycota. the aim of this study was to determine ota levels in spore extracts using hplc and immunoaffinity columns, and to examine the cytotoxicity of pure ota, ota-positive (as-ota(+)) and ota-negative (as-ota(-)) spore extracts, as well as of ap spore extrac ...201526086577
studies on the metabolism of five model drugs by fungi colonizing cadavers using lc-esi-ms/ms and gc-ms analysis.it is well-known that cadavers may be colonized by microorganisms, but there is limited information if or to what extent these microbes are capable of metabolizing drugs or poisons, changing the concentrations and metabolic pattern of such compounds in postmortem samples. the aim of the present study was to develop a fungal biotransformation system as an in vitro model to investigate potential postmortem metabolism by fungi. five model drugs (amitriptyline, metoprolol, mirtazapine, promethazine, ...201222825674
eurotium (aspergillus) repens metabolites and their biological activity.eurotium repens mycelium cultivated under static conditions was used to isolate and identify metabolities--echinulin, physcion, erythroglaucin, flavoglaucin and asperentin; the filtrate of the culture yielded asperentin 8-methylether. the broadest biological activity spectrum was displayed by asperentin which had antibacterial and antifungal effects and, at a concentration of 86 microgram/ml, caused 50% mor7 tality in artemia saline larvae. the highest cytotoxicity towards hela cells was found i ...1978105975
eurotium (aspergillus) repens metabolites and their biological activity.eurotium repens mycelium cultivated under static conditions was used to isolate and identify metabolities--echinulin, physcion, erythroglaucin, flavoglaucin and asperentin; the filtrate of the culture yielded asperentin 8-methylether. the broadest biological activity spectrum was displayed by asperentin which had antibacterial and antifungal effects and, at a concentration of 86 microgram/ml, caused 50% mor7 tality in artemia saline larvae. the highest cytotoxicity towards hela cells was found i ...1978105975
molecular screening of xerophilic aspergillus strains producing mycophenolic acid.mycophenolic acid (mpa) is the fungal secondary metabolite displaying several biological properties. up to now, screening of fungal strains producing mpa has mainly been the result of the search of this molecule in their culture medium by chemical methods. here we developed a molecular approach by targeting the expression level of the mpac gene encoding the polyketide synthase, one of the key enzymes involved in the mpa synthesis. thirty xerophilic aspergillus strains were identified using the r ...201728089042
isolation of an antioxidative substance produced by aspergillus repens.the acidic fraction of an extract of the culture liquid of aspergillus repens ma0197 showed strong antioxidative activity when tested by the ferric thiocyanate and tba methods. chromatographic purification of this acidic fraction gave an active substance identified as neoechinulin a. this compound showed higher antioxidative activity than α-tocopherol and could be expected to act as an antioxidant in katsuobushi.199927385574
assemblage of filamentous fungi associated with aculeate hymenopteran brood in reed galls.monotypic stands of common reed and the reed-gall-associated insect assemblages are distributed worldwide. however, fungi associated with these assemblages have not been characterized in detail. here we examined 5200 individuals (12 species) of immature aculeate hymenopterans or their parasitoids collected at 34 sampling sites in central europe. we noticed fungal outgrowth on exoskeletons of 83 (1.60%) larvae and pupae. the most common host was eudominant pemphredon fabricii. however, the less a ...201626706117
effect of water activity and temperature on the growth of eurotium species isolated from animal feeds.xerophilic fungi represent a serious problem due to their ability to grow at low water activities causing the spoiling of low and intermediate moisture foods, stored goods and animal feeds, with the consequent economic losses.201729137926
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