| genomic variance of the 2019-ncov coronavirus. | there is a rising global concern for the recently emerged novel coronavirus (2019-ncov). full genomic sequences have been released by the worldwide scientific community in the last few weeks to understand the evolutionary origin and molecular characteristics of this virus. taking advantage of all the genomic information currently available, we constructed a phylogenetic tree including also representatives of other coronaviridae, such as bat coronavirus (bcov) and severe acute respiratory syndrom ... | 2020 | 32027036 |
| cross-species transmission of the newly identified coronavirus 2019-ncov. | the current outbreak of viral pneumonia in the city of wuhan, china, was caused by a novel coronavirus designated 2019-ncov by the world health organization, as determined by sequencing the viral rna genome. many initial patients were exposed to wildlife animals at the huanan seafood wholesale market, where poultry, snake, bats, and other farm animals were also sold. to investigate possible virus reservoir, we have carried out comprehensive sequence analysis and comparison in conjunction with re ... | 2020 | 31967321 |
| epitope mapping and cellular localization of swine acute diarrhea syndrome coronavirus nucleocapsid protein using a novel monoclonal antibody. | a swine acute diarrhea syndrome coronavirus (sads-cov) that causes severe diarrhea in suckling piglets was identified in southern china in 2017. to develop an antigen that is specific, sensitive, and easy to prepare for serological diagnosis, antigenic sites in the sads-cov nucleocapsid (n) protein were screened. we generated and characterized an n-reactive monoclonal antibody (mab) 3e9 from mice immunized with recombinant n protein. through fine epitope mapping of mab 3e9 using a panel of eukar ... | 2019 | 31518629 |
| characterization of a novel bat-hku2-like swine enteric alphacoronavirus (seacov) infection in cultured cells and development of a seacov infectious clone. | swine enteric alphacoronavirus (seacov), also known as swine acute diarrhea syndrome coronavirus (sads-cov), belongs to the species rhinolophus bat coronavirus hku2. herein, we report on the primary characterization of seacov in vitro. four antibodies against the seacov spike, membrane, nucleocapsid and nonstructural protein 3 capable of reacting with viral antigens in seacov-infected vero cells were generated. we established a dna-launched seacov infectious clone based on the cell adapted passa ... | 2019 | 31419711 |
| retrospective detection and phylogenetic analysis of swine acute diarrhoea syndrome coronavirus in pigs in southern china. | swine acute diarrhoea syndrome coronavirus (sads-cov), a novel coronavirus, was first discovered in southern china in january 2017 and caused a large scale outbreak of fatal diarrheal disease in piglets. here, we conducted a retrospective investigation of 236 samples from 45 swine farms with a clinical history of diarrheal disease to evaluate the emergence and the distribution of sads-cov in pigs in china. our results suggest that sads-cov has emerged in china at least since august 2016. meanwhi ... | 2019 | 30171801 |