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isolation of a coronavirus from a green-cheeked amazon parrot (amazon viridigenalis cassin).a virus (av71/99) was isolated from a green-cheeked amazon parrot by propagation and passage in both primary embryo liver cells derived from blue and yellow macaw (ara ararauna) embryos and chicken embryo liver cells. electron microscopic examination of cytopathic agents derived from both types of cell cultures suggested that it was a coronavirus. this was confirmed using a pan-coronavirus reverse transcriptase polymerase chain reaction that amplified part of gene 1 that encodes the rna-dependen ...200616595304
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