ranavirus phylogeny and differentiation based on major capsid protein, dna polymerase and neurofilament triplet h1-like protein this study, we developed new methods for differentiation of ranaviruses based on polymerase chain reaction and restriction enzyme analysis of dna polymerase and neurofilament triplet h1-like (nf-h1) protein gene. using these methods, we were able to differentiate the 6 known ranaviruses--bohle iridovirus (biv), european catfish virus (ecv), epizootic haematopoietic necrosis virus (ehnv), european sheatfish virus (esv), frog virus 3 (fv3) and singapore grouper iridovirus (sgiv)--with 3 less ch ...200919694168
quantitation of ranaviruses in cell culture and tissue samples.a quantitative real-time pcr (qpcr) based on a standard curve was developed for detection and quantitation of ranaviruses. the target gene for the qpcr was viral dna polymerase (dnapol). all ten ranavirus isolates studied (epizootic haematopoietic necrosis virus, ehnv; european catfish virus, ecv; european sheatfish virus, esv; frog virus 3, fv3; bohle iridovirus, biv; doctor fish virus, dfv; guppy virus 6, gv6; pike-perch iridovirus, ppiv; rana esculenta virus italy 282/i02, rev282/i02 and shor ...201021087639
genomic sequencing of ranaviruses isolated from edible frogs (pelophylax esculentus).ranaviruses were isolated from wild edible frogs (pelophylax esculentus) during epizootics in denmark and italy. phylogenomic analyses revealed that these isolates are closely related and belong to a clade of ranaviruses that includes the andrias davidianus ranavirus (adrv), common midwife toad ranavirus (cmtv), testudo hermanni ranavirus (thrv), and pike-perch iridovirus (ppiv).201728935748
susceptibility of the european common frog rana temporaria to a panel of ranavirus isolates from fish and amphibian hosts.ranaviruses are an emerging group of viruses and have been implicated in an increase of epidemics in susceptible species. they have a wide host range, infecting fish, amphibians and reptiles, with some isolates able to infect multiple species from different animal classes. whilst some information exists on the pathogenicity of ranaviruses to novel hosts, there is none on the pathogenicity of fish ranaviruses to amphibians; this information is needed to develop measures to prevent the further spr ...201323574703
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