Publications

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susceptibility of black bullhead ameiurus melas to a panel of ranavirus isolates.ranaviruses are considered a serious threat to lower vertebrates, including fish, amphibians and reptiles. however, epidemiological data on these agents are lacking, and further investigations are needed to understand the role of carriers and to update the list of susceptible hosts. we carried out various experimental infections under controlled conditions to contribute to the current knowledge on the susceptibility of black bullhead ameiurus melas to european catfish virus (ecv) and other ranav ...201020815324
quantitation of ranaviruses in cell culture and tissue samples.a quantitative real-time pcr (qpcr) based on a standard curve was developed for detection and quantitation of ranaviruses. the target gene for the qpcr was viral dna polymerase (dnapol). all ten ranavirus isolates studied (epizootic haematopoietic necrosis virus, ehnv; european catfish virus, ecv; european sheatfish virus, esv; frog virus 3, fv3; bohle iridovirus, biv; doctor fish virus, dfv; guppy virus 6, gv6; pike-perch iridovirus, ppiv; rana esculenta virus italy 282/i02, rev282/i02 and shor ...201021087639
selected emerging diseases of amphibia.this review summarizes the most recent updates on emerging infectious diseases of amphibia. a brief summary of batrachochytrium dendrobatidis history, epidemiology, pathogenesis, life cycle, diagnosis, treatment, and biosecurity is provided. ambystoma tigrinum virus, common midwife toad virus, frog virus 3, rana grylio virus, rana catesbeiana ranavirus, mahaffey road virus, rana esculenta virus, bohle iridovirus, and tiger frog virus ranaviruses are extensively reviewed. emerging bacterial patho ...201323642863
susceptibility of the european common frog rana temporaria to a panel of ranavirus isolates from fish and amphibian hosts.ranaviruses are an emerging group of viruses and have been implicated in an increase of epidemics in susceptible species. they have a wide host range, infecting fish, amphibians and reptiles, with some isolates able to infect multiple species from different animal classes. whilst some information exists on the pathogenicity of ranaviruses to novel hosts, there is none on the pathogenicity of fish ranaviruses to amphibians; this information is needed to develop measures to prevent the further spr ...201323574703
ranavirus phylogeny and differentiation based on major capsid protein, dna polymerase and neurofilament triplet h1-like protein genes.in this study, we developed new methods for differentiation of ranaviruses based on polymerase chain reaction and restriction enzyme analysis of dna polymerase and neurofilament triplet h1-like (nf-h1) protein gene. using these methods, we were able to differentiate the 6 known ranaviruses--bohle iridovirus (biv), european catfish virus (ecv), epizootic haematopoietic necrosis virus (ehnv), european sheatfish virus (esv), frog virus 3 (fv3) and singapore grouper iridovirus (sgiv)--with 3 less ch ...200919694168
comparison of european systemic piscine and amphibian iridoviruses with epizootic haematopoietic necrosis virus and frog virus 3.iridovirus-like agents isolated from systemic infected fish (silurus glanis, sfir; ictalurus melas, cfir i, cfir ii, cfir iii) and from frogs (rana esculenta, reir) in europe, epizootic haematopoietic necrosis virus (ehnv) isolated in australia from redfin perch (perca fluviatilis), and frog virus 3 (fv 3) isolated from frogs (rana pipiens) in the usa were investigated by electron microscopy, polypeptide composition, immunofluorescence, restriction endonuclease digestion, southern-blot hybridiza ...19989719770
characterization of an iridescent virus isolated from gryllus bimaculatus (orthoptera: gryllidae).we have isolated an iridescent virus from commercially produced colonies of gryllus bimaculatus in germany, which showed apparent mortality. transmission electron microscopy studies on adult cricket specimens revealed the paracrystalline assembly of icosahedral virus particles in the cytoplasm of hypertrophied abdominal fat body cells. the infecting agent could be cultivated in the lepidopteran cell line sf-9, where it caused cytopathogenic effects such as cell hypertrophy, cytoplasmic vacuoliza ...200111161994
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