| ranavirus-associated mass mortality in wild amphibians, the netherlands, 2010: a first report. | in 2010, a mass die-off of over 1000 wild water frogs (pelophylax spp.) and at least 10 common newts (lissotriton vulgaris) occurred in a pond in the netherlands. haemorrhagic disease with hepatomegaly and splenomegaly was evident. microscopically, multiple organs presented cells with multifocal intracytoplasmic inclusion bodies, in which ranavirus-like particles were demonstrated ultrastructurally. all specimens examined tested positive for ranavirus by pcr. the sequence obtained showed a 100% ... | 2011 | 21955440 |