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life cycle of eimeria krijgsmanni-like coccidium in the mouse (mus musculus).life cycle of eimeria krijgsmanni-like coccidium isolated from the feces of naturally infected mice purchased from commercial sources was examined. the parasite was purified by single oocyst isolation and maintained by passage in the mice before experiments. the sporulated oocysts were ovoid or ellipsoid, measuring 19.3 x 14.8 microm on average. one or two small polar granules were present. micropyle and oocyst residuum were absent. sporocysts were ellipsoid, measuring 11.6 x 7.2 microm on avera ...200616679723
evaluation of eimeria krijgsmanni as a murine model for testing the efficacy of anti-parasitic agents.murine eimeria spp. have been used as effective models of disease instead of large mammalian hosts such as cattle. we attempted to establish in vivo and in vitro assays using a murine intestinal protozoan, eimeria krijgsmanni, which we previously isolated, to test anti-parasitic agents. consequently, when mice were treated with sulfur drugs or toltrazuril, which are commercially available for livestock. furthermore, sporulated oocysts and excysted sporozoites of e. krijgsmanni were treated with ...201526203984
course of induced infection by eimeria krijgsmannni in immunocompetent and immunodeficient mice.recently, we have demonstrated the utility of eimeria krijgsmanni as a novel mouse eimerian parasite for elucidating the biological diversity. the parasite showed notable infectivity to mice with various levels of immune status and susceptibility to antimicrobial agents including coccidiostat. however, the detailed lifecycle of e. krijgsmanni had not yet been determined and this information was lacking in discussion of previous findings. in the present study, we clarified the morphological chara ...201626377842
host specificity and in vivo infectivities of the mouse coccidian parasites eimeria krijgsmanni.in the present study, infection experiments of e. krijgsmanni using various hosts were conducted to elucidate the host specificity among some animals and the infectivity to mouse strains. according to the results, the infection was not found in most animals, except for rats, in which some oocyst shedding was detected, and there was no significant difference in infectivity among mouse strains. additionally, oocyst shedding was hardly detectable in a secondary infection to immunocompetent mice, al ...201424827108
molecular and phylogenetic characterizations of an eimeria krijgsmanni yakimoff & gouseff, 1938 (apicomplexa: eimeriidae) mouse intestinal protozoan parasite by partial 18s ribosomal rna gene sequence analysis.previously, we characterized an undocumented strain of eimeria krijgsmanni by morphological and biological features. here, we present a detailed molecular phylogenetic analysis of this organism. namely, 18s ribosomal rna gene (rdna) sequences of e. krijgsmanni were analyzed to incorporate this species into a comprehensive eimeria phylogeny. as a result, partial 18s rdna sequence from e. krijgsmanni was successfully determined, and two different types, type a and type b, that differed by 1 base p ...201424780139
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