Publications

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use of an in vitro culture system to detect theileria equi strains from infected equids and/or reservoirs.a horse erythrocyte culture technique, partly modifying that originally developed by holman, was used to detect the presence of theileria equi strains in 12 horse and 2 mule blood samples. the animals were placed into four groups on the basis of their case history and laboratory test results: the mules and two horses were considered as infected and included in the 'recent infection' group, four horses with a history of past infection were included in the 'past infection' group and four animals s ...200620429061
seroprevalence and risk factors associated with babesia caballi and theileria equi infection in equids.a cross-sectional study was carried out on equids (horses, mules and donkeys) in andalusia, southern spain, to assess the level of exposure to equine piroplasmosis and to investigate risk factors associated with these infections. at least one animal seropositive for theileria equi and/or babesia caballi was detected in 222/380 (58.4%) herds sampled by competitive inhibition elisas. the seroprevalences for b. caballi and t. equi were 13.2% and 56.1%, respectively; there was serological evidence o ...201322784418
seroprevalence of babesia caballi and theileria equi in five draught equine populated metropolises of punjab, pakistan.equine piroplasmosis (ep) caused by intraerythrocytic parasites (theileria equi and babesia caballi) is an emerging equine disease of world-wide distribution. in pakistan, the prevalence and incidence of ep are unknown. in order to obtain the first insights into the prevalence of the disease, a total of 430 equids, including 33 mules, 65 horses and 332 donkeys, aging from ≤ 5 to ≥ 10 years of either sex, from five metropolises of punjab, pakistan, were serologically tested for the presence of an ...201424582524
multiplex pcr for detection of trypanosoma evansi and theileria equi in equids of punjab, india.multiplex pcr for simultaneous detection of trypanosoma evansi and theileria equi in single-step reaction was optimized and employed on 108 equids (99 horses and 9 donkeys/mules) blood samples collected from two agro-climatic zones (sub-mountain undulating zone and undulating plain zone) of punjab to evaluate the status of concurrent infection and associated risk factors. the amplification products of 257 and 709 bp targeting repetitive nucleotide sequence of variable surface glycoproteins of t. ...201526070973
seroprevalence of equine piroplasms and host-related factors associated with infection in greece.serum samples were collected from a total of 544 equids that included 524 horses, 13 mules, and 7 ponies from various regions of mainland greece and were examined by competitive-inhibition elisa (celisa) to evaluate the level of exposure of greek equids to theileria (babesia) equi and/or babesia caballi, the causative agents of piroplasmosis. association between seropositivity and host-related factors of species, gender, age, origin, activity and location were investigated. the overall seropreva ...201020138434
seroprevalence of equine babesiosis in the black sea region of turkey.the prevalence of theileria equi and babesia caballi was determined in equid blood samples in five provinces of the black sea region of turkey by using the indirect fluorescent antibody test (ifat). of 153 samples, 53 (34.6%) and 33 (21.5%) were seropositive to b. caballi and t. equi, respectively. in addition, 8 (5.2%) of samples were seropositive to both t. equi and b. caballi. anti t. equi and b. caballi antibodies were detected in all five regions. the prevalence of b. caballi was higher tha ...200818234550
spatial distribution, risk factors and haemato-biochemical alterations associated with theileria equi infected equids of punjab (india) diagnosed by indirect elisa and nested pcr.equine piroplasmosis is a febrile, tick-borne disease of equids predominately caused by obligatory intra-erythrocytic protozoa theileria equi in the indian sub-continent. a cross-sectional study was carried out on 464 equids (426 horses and 38 donkeys/mules) in punjab, india to assess the level of exposure to equine piroplasmosis by 18s rrna gene nested polymerase chain reaction (npcr) and equine merozoite antigen-2 (ema2) indirect-elisa (enzyme linked immunosorbent assay), to investigate risk f ...201626747007
vector ecology of equine piroplasmosis.equine piroplasmosis is a disease of equidae, including horses, donkeys, mules, and zebras, caused by either of two protozoan parasites, theileria equi or babesia caballi. these parasites are biologically transmitted between hosts via tick vectors, and although they have inherent differences they are categorized together because they cause similar pathology and have similar morphologies, life cycles, and vector relationships. to complete their life cycle, these parasites must undergo a complex s ...201525564746
babesiosis in china.the importance of babesiosis in livestock in china is discussed and mainly focused on bovine and equine babesiosis. babesiosis is still one of the most important diseases affecting livestock and has caused great economic loss. nine species of babesia have been recognized in livestock: b. bigemina, b. bovis, b. major, b. motasi, b. ovis, b. perroncitoi, b. trautmanni, b. equi (theileria equi), b. caballi. the distribution of babesia follows the distribution of the tick vectors. the main vectors o ...19979512738
theileria equi and babesia caballi infection of equids in punjab, india: a serological and molecular survey.a cross-sectional study was conducted in submountain undulating, undulating plain, western and western plain agro-climatic zones of punjab province, india, to determine the prevalence, agreement between diagnostic tests and associated related risk factors of theileria equi and babesia caballi infection in equids (horses, donkey, mules). an overall prevalence of 14.14 and 0.0% of t. equi and b. caballi was recorded by multiplex polymerase chain reaction targeting 18s ribosomal rna (rrna) for both ...201626387094
microscopic and molecular detection of theileria (babesia) equi infection in equids of kurdistan province, iran.equine piroplasmosis (ep) is the cause of persistent tick-borne infection with no symptoms, but the most important problem of ep is due to the persistent carrier state. carrier animals to babesia (theileria) equi (laveran 1901) and b. caballi (nuttall, 1910) infestation could be identified by extremely sensitive pcr-based method. the purpose of this study was to identify the causative agents of equine piroplasmosis based on molecular and microscopic assays in equids from kurdistan province, iran ...027095973
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