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new foci of haemaphysalis punctata and dermacentor reticulatus in the netherlands.in 2014 haemaphysalis punctata was found in several locations on the mainland of the netherlands for the first time since 1897. in the same areas dermacentor reticulatus and ixodes ricinus were found. haemaphysalis punctata and d. reticulatus were tested for presence of babesia spp. and rickettsia spp. by pcr. babesia spp. and spotted fever rickettsiae were not detected in any of the collected h. punctata, while several d. reticulatus (6%) collected from the same areas were found to be positive ...201626726807
isolation of rickettsia helvetica from ticks in slovakia.to date, only three rickettsial species have been found in ticks in slovakia by serological and/or molecular-biological techniques, namely rickettsia slovaca, candidatus rickettsia irs, and rickettsia raoultii. recently, we succeeded in isolation of the forth species, rickettsia helvetica from ixodes ricinus, the most frequent tick in slovakia. the isolation, positive for 10% of tested ticks, was performed on xtc cells by the shell-vial technique, gimenez staining and light microscopy. the infec ...201223043605
new records of spotted fever group rickettsiae in baltic region.tick-borne rickettsiae are considered to be emerging, but there is still a lack of data on the occurrence and prevalence of the spotted fever group rickettsiae across europe, especially in the baltic countries. the aim of the present study was to investigate the presence of rickettsia spp. in dermacentor reticulatus and ixodes ricinus ticks, as well as to determine their prevalence in various regions of lithuania and latvia. the prevalence of rickettsia spp. in geographically distinct population ...201626383013
prevalence and diversity of human pathogenic rickettsiae in urban versus rural habitats, hungary.tick-borne rickettsioses belong to the important emerging infectious diseases worldwide. we investigated the potential human exposure to rickettsiae by determining their presence in questing ticks collected in an urban park of budapest and a popular hunting and recreational forest area in southern hungary. differences were found in the infectious risk between the two habitats. rickettsia monacensis and rickettsia helvetica were identified with sequencing in questing ixodes ricinus, the only tick ...201626613759
the role of particular tick developmental stages in the circulation of tick-borne pathogens affecting humans in central europe. 3. rickettsiae.ixodes ricinus, dermacentor reticulatus and d. marginatus ticks are the most important vector for rickettsia spp. in central europe. ticks sustain rickettsial transmission cycles transovarially and transstadially, it makes enable the rickettsial circulation in the tick population in the absence of vertebrate competent reservoir. rickettsia helvetica is transmitted by i. ricinus tick; the highest rates of infection are noted in adult females, lower in males and in nymphs. all tick developmental s ...201727614472
isolation and maintenance of rickettsia raoultii in a rhipicephalus sanguineus tick cell line.rickettsia raoultii, a member of the spotted fever group rickettsiae, has been implicated in cases of debonel/tibola/senlat, and has been detected in dermacentor spp. and rhipicephalus pumilio ticks by pcr. r. raoultii has been isolated in mammalian and tick cell lines. this study aimed to isolate r. raoultii from spanish dermacentor marginatus in tick cell lines. a single adult d. marginatus collected from vegetation in la rioja (northen spain) in october 2012 was surface-sterilised, triturated ...201626428859
sympatric occurrence of ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks and rickettsia and babesia species in slovakia.vojka nad dunajom in the south-west of the slovak republic is a locality with sympatric occurrence of 3 species of ticks. this study investigated the spatial distribution of dermacentor reticulatus, ixodes ricinus, and haemaphysalis concinna ticks in this area and determined the prevalence of babesia and rickettsia species in questing adults of these tick species considered as potential risk for humans and animals. ticks were collected by blanket dragging over the vegetation from september 2011 ...201424973275
molecular detection of rickettsia conorii and other zoonotic spotted fever group rickettsiae in ticks, romania.the diverse tick fauna as well as the abundance of tick populations in romania represent potential risks for both human and animal health. spotted fever group (sfg) rickettsiae are recognized as important agents of emerging human tick-borne diseases worldwide. however, the epidemiology of rickettsial diseases has been poorly investigated in romania. in urban habitats, companion animals which are frequently exposed to tick infestation, play a role in maintenance of tick populations and as reservo ...201626507182
molecular evidence for bacterial and protozoan pathogens in hard ticks from romania.the aim of the present study was to provide a preliminary insight into the diversity of tick-borne pathogens circulating at the domestic host-tick interface in romania. for this, feeding and questing ticks were analyzed by real-time polymerase chain reaction (pcr) for the presence of anaplasma phagocytophilum, anaplasma platys, ehrlichia canis, borrelia burgdorferi sensu latu, and by pcr and subsequent sequencing for rickettsia spp., babesia spp. and theileria spp. a total of 382 ticks, encompas ...201323428204
molecular detection of emerging tick-borne pathogens in vojvodina, serbia.ticks play an important role in disease transmission globally due to their capability to serve as vectors for human and animal pathogens. the republic of serbia is an endemic area for a large number of tick-borne diseases. however, current knowledge on these diseases in serbia is limited. the aim of this study was to investigate the presence of new emerging tick-borne pathogens in ticks collected from dogs and the vegetation from different parts of vojvodina, serbia. a total of 187 ticks, includ ...201626565929
emergence of tick-borne pathogens (borrelia burgdorferi sensu lato, anaplasma phagocytophilum, ricketsia raoultii and babesia microti) in the kyiv urban parks, ukraine.to date, only limited data about the presence of ticks and circulation of tick-borne pathogens in urban parks of kyiv in northern ukraine are available. in total, 767 ticks (696 ixodes ricinus and 69 dermacentor reticulatus) collected in seven urban parks and one suburban oak wood park in kyiv were individually analyzed by the pcr assays. tick-borne pathogens, namely spirochetes from borrelia burgdorferi sensu lato complex, anaplasma phagocytophilum, and babesia microti, were detected in 11.1% o ...201727923669
prevalence of infections and co-infections with 6 pathogens in dermacentor reticulatus ticks collected in eastern poland.occurrence of co-infections with various pathogens in ixodid ticks creates a risk of increased severity of tick-borne diseases in humans and animals exposed to bite of the ticks carrying multiple pathogens. accordingly, co-infections in ticks were subject of numerous analyses, but almost exclusively with regard to ixodes ricinus complex whereas potential tick vectors belonging to other genera were much less studied. taking into consideration the role of dermacentor reticulatus in the transmissio ...201728378977
kampinos national park: a risk area for spotted fever group rickettsioses, central poland?ixodid ticks are important vectors of a variety of bacterial and protozoan pathogens which cause infections in humans. in this study, altogether 1041 questing ixodes ricinus (n = 305) and dermacentor reticulatus ticks (n = 736), sympatrically occurring in kampinos national park (kpn), central-east poland, were analyzed by pcr for rickettsia species. overall, the pathogen prevalence in ticks was 27.5 % for i. ricinus and 42.8 % for d. reticulatus. sequencing analysis showed that the first tick sp ...201627631765
spotted fever group rickettsiae in dermacentor reticulatus and haemaphysalis punctata ticks in the uk.spotted fever group (sfg) rickettsiae have recently been identified for the first time in uk ticks. this included the findings of rickettsia helvetica in ixodes ricinus and rickettsia raoultii in dermacentor reticulatus. this paper further investigates the occurrence of sfg rickettsiae in additional geographically distinct populations of d. reticulatus, and for the first time, investigates the occurrence of sfg rickettsiae in uk populations of haemaphysalis punctata ticks.201323870197
spotted fever rickettsiosis caused by rickettsia raoultii--case report.we report the case of rickettsial eschar-associated spotted fever, most probable due to rickettsia raoultii, an emerging pathogen, which was previously described in patients with tick-borne lymphadenopathy (tibola), also called dermacentor-borne necrosis erythema and lymphadenopathy (debonel). the pathogenicity of r. raoultii is not well established. the survey of ticks from poland (ixodes ricinus and dermacentor reticulatus) revealed that r. raoultii occur in all regions of poland and predomina ...201223101229
rickettsia slovaca and rickettsia raoultii in dermacentor marginatus and dermacentor reticulatus ticks from slovak republic.rickettsiae, obligate intracellular gram-negative bacteria, responsible for mild to severe diseases in humans are associated with arthropod vectors. dermacentor marginatus and dermacentor reticulatus are known vectors of rickettsia slovaca and rickettsia raoultii distributed across europe. a total of 794 d. marginatus, d. reticulatus and ixodes ricinus adult ticks were collected from the vegetation, removed from horses, sheep, goats and dogs in slovakia. the dna of rickettsia sp. was found in 22 ...201222392435
scalp eschar and neck lymphadenopathy after tick bite: an emerging syndrome with multiple causes.the clinical and epidemiological features of 56 patients with scalp eschar associated with neck lymphadenopathy after a tick bite (senlat) syndrome were evaluated at the national french rickettsial center. eschar swabs, crusts, and biopsies as well as ticks and blood samples were acquired for molecular and serological assays. senlat predominantly affects children (pā€‰<ā€‰0.05), followed by 40- to 70-year-olds, and it is found mostly in women (pā€‰<ā€‰0.05). the seasonal distribution has two peaks: one ...201424682865
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