Publications

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The effect of induced queen replacement on Nosema spp. infection in honey bee (Apis mellifera iberiensis) colonies.Microsporidiosis of adult honeybees caused by Nosema apis and Nosema ceranae is a common worldwide disease with negative impacts on colony strength and productivity. Few options are available to control the disease at present. The role of the queen in bee population renewal and the replacement of bee losses due to Nosema infection is vital to maintain colony homeostasis. Younger queens have a greater egg laying potential and they produce a greater proportion of uninfected newly eclosed bees to c ...201122118366
comparative study of nosema ceranae (microsporidia) isolates from two different geographic origins.the intestinal honey bee parasite nosema ceranae (microsporidia) is at the root of colony losses in some regions while in others its presence causes no direct mortality. this is the case for spain and france, respectively. it is hypothesized that differences in honey bee responses to n. ceranae infection could be due to the degree of virulence of n. ceranae strains from different geographic origins. to test this hypothesis, we first performed a study to compare the genetic variability of an rdna ...201323031340
screening alternative therapies to control nosemosis type c in honey bee (apis mellifera iberiensis) colonies.nosemosis type c caused by the microsporidium nosema ceranae is one of the most widespread of the adult honey bee diseases, and due to its detrimental effects on both strength and productivity of honey bee colonies, an appropriate control of this disease is advisable. fumagillin is the only veterinary medicament recommended by the world organization for animal health (oie) to suppress infections by nosema, but the use of this antibiotic is prohibited in the european union and few alternatives ar ...201324148868
virulence and polar tube protein genetic diversity of nosema ceranae (microsporidia) field isolates from northern and southern europe in honeybees (apis mellifera iberiensis).infection of honeybees by the microsporidian nosema ceranae is considered to be one of the factors underlying the increased colony losses and decreased honey production seen in recent years. however, these effects appear to differ in function of the climatic zone, the distinct beekeeping practices and the honeybee species employed. here, we compared the response of apis mellifera iberiensis worker bees to experimental infection with field isolates of n. ceranae from an oceanic climate zone in no ...201424992540
characterization of nosema ceranae genetic variants from different geographic origins.in recent years, large-scale colony losses of honey bees (apis mellifera) have been reported and the infection with the microsporidia nosema ceranae has been involved. however, the effect of n. ceranae at the colony level and its role in colony losses vary in different geographic areas. this difference may be related to the presence of multiple n. ceranae genetic variants resulting in different biological consequences. in this study, we analyzed the genetic diversity of 75 n. ceranae samples obt ...201727837253
liquid chromatography coupled to ion trap-tandem mass spectrometry to evaluate juvenile hormone iii levels in bee hemolymph from nosema spp. infected colonies.it has been described a fast, simple and sensitive liquid chromatography-tandem mass spectrometry (lc-ms/ms) method to measure juvenile hormone iii (jh iii), which was used to study of the effects of nosema spp. infection on jh iii levels in bee hemolymph. honey bee hemolymph was extracted by centrifugation and mixed with a solution of phenylthiourea in methanol. this mixture was then centrifuged and the supernatant removed and evaporated to dryness. the residue was reconstituted in methanol con ...201222664054
nosema spp. infection and its negative effects on honey bees (apis mellifera iberiensis) at the colony level.nosemosis caused by the microsporidia nosema apis and nosema ceranae are among the most common pathologies affecting adult honey bees. n. apis infection has been associated with a reduced lifespan of infected bees and increased winter mortality, and its negative impact on colony strength and productivity has been described in several studies. by contrast, when the effects of nosemosis type c, caused by n. ceranae infection, have been analysed at the colony level, these studies have largely focus ...201323574888
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