postmortem diagnosis of morbillivirus infection in bottlenose dolphins (tursiops truncatus) in the atlantic and gulf of mexico epizootics by polymerase chain reaction-based assay.lung tissue from 39 bottlenose dolphins (tursiops truncatus) found dead off the u.s. atlantic and gulf of mexico coasts from 1987 to 1994 was examined for the presence of morbillivirus using a reverse transcriptase polymerase chain reaction (rt-pcr) technique. of the atlantic cases examined, six of six were positive using this assay; 18 of 25 gulf of mexico cases with amplifiable rna also were found to be positive, and eight additional specimens had no amplifiable rna. the rt-pcr allowed the dia ...19958592367
serological evidence of morbillivirus infection in small cetaceans from the southeast pacific.the presence of morbillivirus-specific serum antibodies was examined by an indirect enzyme linked immunosorbent assay (ielisa) and virus neutralization tests in serum samples from 30 dusky dolphins (lagenorhynchus obscurus), 8 long-snouted common dolphins (delphinus capensis), 2 inshore and 6 offshore bottlenose dolphins (tursiops truncatus) and 20 burmeister's porpoises (phocoena spinipinnis) taken in fisheries off central peru in 1993-1995. the sera from six dusky dolphins, one common dolphin ...19989549850
dermatitis with invasive ciliated protozoa in dolphins that died during the 1987-1988 atlantic bottlenose dolphin morbilliviral epizootic.dermatitis with intradermal cilated protozoa was identified in 18 of 95 (19%) atlantic bottlenose dolphins (tursiops truncatus) that died during the 1987-1988 atlantic-dolphin morbillivirus epizootic. the lesions were characterized by focally extensive suppurative and histiocytic dermatitis and cellulitis with ulceration and variable numbers of dermal and hypodermal ciliates. vasculitis, thrombosis, and/or intravascular ciliates were rarely present. in one dolphin, there was an associated lympha ...199910098649
an insight into the epidemiology of dolphin morbillivirus worldwide.serum samples from 288 cetaceans representing 25 species and originating from 11 different countries were collected between 1995 and 1999 and examined for the presence of dolphin morbillivirus (dmv)-specific antibodies by an indirect elisa (ielisa) (n = 267) or a plaque reduction assay (n = 21). a total of 35 odontocetes were seropositive: three harbour porpoises (phocoena phocoena) and a common dolphin (delphinus delphis) from the northeastern (ne) atlantic, a bottlenose dolphin (tursiops trunc ...200111390111
a dna vaccine against dolphin morbillivirus is immunogenic in bottlenose dolphins.the immunization of exotic species presents considerable challenges. nevertheless, for facilities like zoos, animal parks, government facilities and non-profit conservation groups, the protection of valuable and endangered species from infectious disease is a growing concern. the rationale for immunization in these species parallels that for human and companion animals; to decrease the incidence of disease. the u.s. navy marine mammal program, in collaboration with industry and academic partners ...200717706293
fatal cetacean morbillivirus infection in an australian offshore bottlenose dolphin (tursiops truncatus).a juvenile offshore bottlenose dolphin (tursiops truncatus) was found stranded with neurological signs and unable to swim or float unassisted. it subsequently died, succumbing to a combination of severe pneumonia and encephalitis. morbillivirus serum neutralisation test serology was positive (titre 1:16) for cetacean morbillivirus and negative for both phocine distemper virus and canine distemper virus. there was concurrent thymic and lymph node lymphoid depletion and necrosis, together with int ...201122008125
clinicoimmunopathologic findings in atlantic bottlenose dolphins tursiops truncatus with positive cetacean morbillivirus antibody titers.sera from free-ranging atlantic bottlenose dolphins tursiops truncatus inhabiting the indian river lagoon (irl), florida were tested for antibodies to cetacean morbilliviruses from 2003 to 2007 as part of a multidisciplinary study of individual and population health. a suite of clinicoimmunopathologic variables were evaluated in morbillivirus-seropositive dolphins (n = 14) and seronegative healthy dolphins (n = 49). several important differences were found. serum alkaline phosphatase, creatine p ...201122303627
diagnosis of cetacean morbillivirus: a sensitive one step real time rt fast-pcr method based on sybr(®) green.cetacean morbillivirus (cemv) (family paramyxoviridae, genus morbillivirus) is considered the most pathogenic virus of cetaceans. it was first implicated in the bottlenose dolphin (tursiops truncatus) mass stranding episode along the northwestern atlantic coast in the late 1980s, and in several more recent worldwide epizootics in different odontoceti species. this study describes a new one step real-time reverse transcription fast polymerase chain reaction (real-time rt-fast pcr) method based on ...201526454114
morbillivirus infection in cetaceans stranded along the italian coastline: pathological, immunohistochemical and biomolecular findings.morbilliviruses are recognized as biological agents highly impacting the health and conservation status of free-ranging cetaceans worldwide, as clearly exemplified by the two dolphin morbillivirus (dmv) epidemics of 1990-1992 and 2006-2008 among mediterranean striped dolphins (stenella coeruleoalba). after these two epidemics, morbilliviral infection (mi) cases with peculiar neurobiological features were reported in striped dolphins stranded along the spanish coastline. affected cetaceans showed ...201322921372
dolphin morbillivirus in a cuvier's beaked whale (ziphius cavirostris), italy.dolphin morbillivirus (dmv) has caused several mortality events in mediterranean striped (stenella coeruleoalba) and bottlenose (tursiops truncatus) dolphins populations since 19; in the last 5 years, the virus was reported to infect new hosts in this basin, such as fin whales (balaenoptera physalus), sperm whales (physeter macrocephalus), and even a harbor seal (phoca vitulina). very recently, a calf cuvier's beaked whale (ziphius cavirostris) calf stranded on the southern italian coastline wit ...201728197145
western blot expression of 5-lipoxygenase in the brain from striped dolphins (stenella coeruleoalba) and bottlenose dolphins (tursiops truncatus) with or without encephalitis/meningo-encephalitis of infectious nature.dolphin morbillivirus (dmv), toxoplasma gondii and brucella ceti are pathogens of major concern for wild cetaceans. although a more or less severe encephalitis/meningo-encephalitis may occur in striped dolphins (stenella coeruleoalba) and bottlenose dolphins (tursiops truncatus) infected by the aforementioned agents, almost no information is available on the neuropathogenesis of brain lesions, including the neuronal and non-neuronal cells targeted during infection, along with the mechanisms unde ...201525864766
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