mutability dynamics of an emergent single stranded dna virus in a naïve host.quasispecies variants and recombination were studied longitudinally in an emergent outbreak of beak and feather disease virus (bfdv) infection in the orange-bellied parrot (neophema chrysogaster). detailed health monitoring and the small population size (<300 individuals) of this critically endangered bird provided an opportunity to longitudinally track viral replication and mutation events occurring in a circular, single-stranded dna virus over a period of four years within a novel bottleneck p ...201424416396
differential expression of two isolates of beak and feather disease virus capsid protein in escherichia coli.expression of recombinant beak and feather disease virus (bfdv) capsid-associated protein (cap) has relied on inefficient techniques that typically produce low yields or use specialized expression systems, which greatly increase the cost and expertise required for mass production. an escherichia coli system was used to express recombinant bfdv cap derived from two isolates of bfdv, from a long-billed corella (cacatua tenuirostris) and an orange-bellied parrot (obp; neophema chrysogaster). purifi ...201323403150
evidence of psittacine beak and feather disease virus spillover into wild critically endangered orange-bellied parrots (neophema chrysogaster).we report the recent emergence of a novel beak and feather disease virus (bfdv) genotype in the last remaining wild population of the critically endangered orange-bellied parrot (neophema chrysogaster). this virus poses a significant threat to the recovery of the species and potentially its survival in the wild. we used pcr to detect bfdv in the blood of three psittacine beak and feather disease (pbfd)-affected wild orange-bellied parrot fledglings captured as founders for an existing captive br ...201424484492
rapid genotyping of beak and feather disease virus using high-resolution dna melt curve analysis.beak and feather disease virus (bfdv) is a significant pathogen both for wild and captive psittacine birds globally. genotypic differentiation of bfdv isolates is crucial to establish effective control strategies for the conservation of endangered species and epidemiological investigations of disease outbreaks. the technique developed in this study is a simple, rapid and inexpensive genotyping method for bfdv using pcr and subsequent high-resolution melt (hrm) curve analysis. this was achieved u ...201425102431
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