| rnai-mediated resistance to bean golden mosaic virus in genetically engineered common bean (phaseolus vulgaris). | bean golden mosaic virus (bgmv) is transmitted by the whitefly bemisia tabaci in a persistent, circulative manner, causing the golden mosaic of common bean (phaseolus vulgaris l.). the characteristic symptoms are yellow-green mosaic of leaves, stunted growth, or distorted pods. the disease is the largest constraint to bean production in latin america and causes severe yield losses (40 to 100%). here, we explored the concept of using an rna interference construct to silence the sequence region of ... | 2007 | 17555279 |
| in situ detection of early replication phases of a gemini virus in legume protoplasts. | protoplasts of phaseolus vulgaris l. (top crop), infected with bean golden mosaic virus, were isolated and fixed by various methods for in situ hybridization. an iodine-125 labeled probe was made from the replicative form of the virus. the localization and quantitation was done by autoradiography. cell wall removal lowered the background and allowed a more accurate analysis. rnase was used to eliminate the possibility of hybrids to rna. the evidence suggests a sequence of virus movements startin ... | 1987 | 24248933 |
| stable transformation of phaseolus vulgaris via electric-discharge mediated particle acceleration. | transgenic phaseolus vulgaris or common bean has been produced using electric-discharge particle acceleration. the method uses particle acceleration to introduce dna into bean seed meristems. multiple shoots are then generated and screened to recover transgenic plants at a rate of 0.03% germline transformed plants/shoot. we have been able to recover transgenic plants using both gus and herbicide screening to introduce the gus, bar, and bean golden mosaic virus coat protein genes into the navy be ... | 1993 | 24196855 |
| inheritance of foreign genes in transgenic bean (phaseolus vulgaris l.) co-transformed via particle bombardment. | exploiting the biolistic process we have generated stable transgenic bean (phaseolus vulgaris l.) plants with unlinked and linked foreign genes. co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2s albumin gene isolated from the brazil nut and the antisense sequence of ac1, ac2, ac3 and bc1 genes from the bean golden mosaic geminivirus. the results revealed a co-transformation frequency rang ... | 1996 | 24162211 |
| primers and probes development for specific pcr detection of genetically modified common bean (phaseolus vulgaris) embrapa 5.1. | the genetically modified common bean embrapa 5.1, developed by brazilian agricultural research corporation (embrapa), is the first commercial gm plant produced in latin america. it presents high resistance to the bean golden mosaic virus. in this work, primers and probes targeting a taxon-specific reference dna sequence for the common bean (phaseolus vulgaris l.) and a construct-specific dna sequence of embrapa 5.1 gm common bean were successfully developed. the primers and probes showed high sp ... | 2012 | 22506690 |
| molecular characterization of the first commercial transgenic common bean immune to the bean golden mosaic virus. | golden mosaic of common bean is caused by the bean golden mosaic virus (bgmv). the disease is one of the greatest constraints on bean production in latin america and causes significant yield losses. the rnai concept was explored to silence the rep (ac1) viral gene and a transgenic bean line immune to bgmv upon inoculation at high pressure was previously generated. identification of the transgene insert confirmed the presence of a single locus corresponding to two intact copies of the rnai casset ... | 2013 | 23639387 |
| populational survey of arthropods on transgenic common bean expressing the rep gene from bean golden mosaic virus. | genetically modified (gm) crops is considered the fastest adopted crop technology in the history of modern agriculture. however, possible undesirable and unintended effects must be considered during the research steps toward development of a commercial product. in this report we evaluated effects of a common bean virus resistant line on arthropod populations, considered as non-target organisms. this gm bean line (named m1/4) was modified for resistance against bean golden mosaic virus (bgmv) by ... | 2017 | 24922280 |
| contrasting genetic structure between two begomoviruses infecting the same leguminous hosts. | begomoviruses are whitefly-transmitted, ssdna plant viruses and are among the most damaging pathogens causing epidemics in economically important crops worldwide. wild/non-cultivated plants play a crucial epidemiological role, acting as begomovirus reservoirs and as 'mixing vessels' where recombination can occur. previous work suggests a higher degree of genetic variability in begomovirus populations from non-cultivated hosts compared with cultivated hosts. to assess this supposed host effect on ... | 2014 | 25028472 |
| development of an event-specific hydrolysis probe quantitative real-time polymerase chain reaction assay for embrapa 5.1 genetically modified common bean (phaseolus vulgaris). | a genetically modified (gm) common bean event, namely embrapa 5.1, resistant to the bean golden mosaic virus (bgmv), was approved for commercialization in brazil. brazilian regulation for genetically modified organism (gmo) labeling requires that any food containing more than 1% gmo be labeled. the event-specific polymerase chain reaction (pcr) method has been the primary trend for gmo identification and quantitation because of its high specificity based on the flanking sequence. this work repor ... | 2014 | 25437743 |
| comparative analysis of nutritional compositions of transgenic rnai-mediated virus-resistant bean (event emb-pv051-1) with its non-transgenic counterpart. | golden mosaic is among the most economically important diseases that severely reduce bean production in latin america. in 2011, a transgenic bean event named embrapa 5.1 (emb-pv051-1), resistant to bean golden mosaic virus, was approved for commercial release in brazil. the aim of this study was to measure and evaluate the nutritional components of the beans, as well as the anti-nutrient levels in the primary transgenic line and its derived near-isogenic lines after crosses and backcrosses with ... | 2015 | 25894661 |
| phaseolus immature embryo rescue technology. | predominant among the production constraints of the common bean phaseolus vulgaris are infestation of ascochyta blight, bean golden mosaic virus (bgmv), and bean fly. interbreeding with phaseolus -coccineus l. and/or phaseolus polyanthus greenm has been shown to provide p. vulgaris with greater resistance to these diseases. for interspecific crosses to be successful, it is important to use p. coccineus and p. polyanthus as female parents; this prevents rapid reversal to the recurrent parent p. v ... | 2011 | 21207266 |
| backcross assisted by microsatellite markers in common bean. | the objectives of the present study were to monitor the effect of backcrossing through microsatellite markers and to compare different marker assisted selection strategies. four populations were developed using donor parents resistant to the bean golden mosaic virus and, for all crosses, only individuals resistant to the bean golden mosaic virus were backcrossed. for crosses arc100-4 x dor303 and arc100-4 x phas8328, assisted selection was carried out in the f(2) and f(2)bc(1) generations, while ... | 2008 | 19048479 |
| variability in geminivirus isolates associated with phaseolus spp. in brazil. | abstract bean golden mosaic geminivirus (bgmv) is the single most devastating virus of common beans in the tropical and subtropical americas and the caribbean basin. the bgmv from brazil, named bgmv-bz, is considered distinct from bgmv-pr isolates from puerto rico, guatemala, and the dominican republic because of dna sequence data, the ability to form pseudorecombinants, and mechanical transmissibility properties. in bean-growing areas of brazil, samples were collected from beans, lima beans, an ... | 1999 | 18944768 |
| trans-dominant inhibition of geminiviral dna replication by bean golden mosaic geminivirus rep gene mutants. | abstract geminiviruses are a group of single-stranded dna viruses that cause major losses on a number of important crops throughout the world. bean golden mosaic virus (bgmv) is a typical bipartite, whitefly-transmitted geminivirus that causes a severe disease on beans (phaseolus vulgaris) in the western hemisphere. the lack of natural resistance to geminiviruses has led to attempts to engineer resistance, particularly through the use of pathogen-derived resistance strategies. the rep gene conta ... | 1999 | 18944720 |
| [biological aspects of bemisia tabaci (genn.) b biotype (hemiptera: aleyrodidae) on six bean genotypes]. | the silverleaf whitefly is one of the most harmful pests that attack bean crops, mainly for extracting large quantities of phloem sap and transmitting the bean golden mosaic virus. resistant germoplasm plants can be an important method for controlling this pest. the biological aspects of bemisia tabaci b biotype on bean (phaseolus vulgaris) genotypes were evaluated. the tests were conducted under laboratory conditions, with the following genotypes: arc 1, arc 3s, arc 5s, g13028, g11056 and porri ... | 2008 | 18506300 |
| isolation and characterization of virus-specific double-stranded dna from tissues infected by bean golden mosaic virus. | a double-stranded (ds) dna which may be a replication intermediate was isolated from bean (phaseolus vulgaris l. "top crop") leaves systemically infected with bean golden mosaic virus, a whitefly-transmitted plant virus with a genome of circular single-stranded (ss) dna. the isolation method used phenol/chloroform extraction, hydroxyapatite column chromatography, and rate-zonal centrifugation. the dsdna had sequences complementary to those of viral dna. the guanine-plus-cytosine content was 35%, ... | 1981 | 16593050 |
| bipartite geminivirus host adaptation determined cooperatively by coding and noncoding sequences of the genome. | bipartite geminiviruses are small, plant-infecting viruses with genomes composed of circular, single-stranded dna molecules, designated a and b. although they are closely related genetically, individual bipartite geminiviruses frequently exhibit host-specific adaptation. two such viruses are bean golden mosaic virus (bgmv) and tomato golden mosaic virus (tgmv), which are well adapted to common bean (phaseolus vulgaris) and nicotiana benthamiana, respectively. in previous studies, partial host ad ... | 2000 | 11080490 |
| effect of silver reflective mulch and a summer squash trap crop on densities of immature bemisia argentifolii (homoptera: aleyrodidae) on organic bean. | polyethylene mulch with a reflective silver stripe and a yellow summer squash, cucurbita pepo l., trap crop were tested alone and in combination as tactics to reduce densities of bemisia argentifolii bellows & perring eggs and nymphs, and incidence of bean golden mosaic geminivirus on snap bean, phaseolus vulgaris l. egg densities were consistently higher on squash than on bean, but egg densities and virus incidence were not lower on bean grown with squash than on bean grown in monoculture. silv ... | 2000 | 10902322 |
| mutational analysis of the putative nicking motif in the replication-associated protein (ac1) of bean golden mosaic geminivirus. | geminiviruses are circular single-stranded dna viruses that replicate by a rolling circle mechanism involving the viral-encoded ac1 protein. dna nicking is necessary both for initiating replication of the covalently closed double-stranded dna templates and for releasing unit-length monomers. the effects of mutations in a putative nicking motif (k101 a y i d k106; e. v. koonin and t. v. ilyina, j. gen. virol. 73:2763-2766, 1992) of the ac1-derived protein for bean golden mosaic geminivirus isolat ... | 1996 | 8810074 |
| whitefly transmission and efficient ssdna accumulation of bean golden mosaic geminivirus require functional coat protein. | bean golden mosaic geminivirus (bgmv) has a bipartite genome composed of two circular ssdna components (dna-a and dna-b) and is transmitted by the whitefly, bemisia tabaci. dna-a encodes the viral replication proteins and the coat protein. to determine the role of bgmv coat protein systemic infection and whitefly transmission, two deletions and a restriction fragment inversion were introduced into the bgmv coat protein gene. all three coat protein mutants produced systemic infections when coinoc ... | 1994 | 8091659 |
| infection of phaseolus vulgaris by bean golden mosaic virus: ultrastructural aspects. | | 1978 | 685179 |