| immunokinetics of equine herpesvirus 1 in donkey mares: suppression of secondary cell-mediated response. | to study the immunokinetics of equine herpesvirus 1 (ehv1), donkey mares were immunised with a laboratory strain of ehv1, or with recommended doses of pneumabort-k vaccine (ehv1 army 183 strain, formalin-inactivated, with an oil adjuvant) and a booster was given after three months. humoral immune responses were studied by employing a virus neutralisation (vn) test. a leucocyte migration inhibition test (lmit) was employed for the assay of cellular immune responses. the vn antibody titre reached ... | 1992 | 1335311 |
| comparative studies of the proteins of equine herpesviruses 4 and 1 and asinine herpesvirus 3: antibody response of the natural hosts. | proteins of purified virions of equine herpesvirus 4 (ehv-4; equine rhinopneumonitis), ehv-1 (equine abortion virus) and asinine herpesvirus 3 (ahv-3) were compared by metabolic labelling with [35s]methionine or [14c]glucosamine during growth of low passage virus in natural host cells (horse or donkey) and high passage virus in an appropriate cell line and analysis by sds-page. approximately 25 different proteins (mr 300k to 21.5k) were clearly resolved for each virus. the three viruses had simi ... | 1990 | 2170572 |
| the nucleotide sequence of asinine herpesvirus 3 glycoprotein g indicates that the donkey virus is closely related to equine herpesvirus 1. | the nucleotide sequence of the glycoprotein g (gg) homologue of asinine herpesvirus 3 (ahv3), a respiratory alphaherpesvirus of donkeys, was determined. the ahv3 gg gene consists of 1233 base pairs (bp) and codes for a predicted protein of 411 amino acids. this is identical in size to the equine herpesvirus 1 (ehv1) gg gene and 6 amino acids longer than the equine herpesvirus 4 (ehv4) gg gene. the predicted amino acid sequence of ahv3 gg has characteristics of a class 1 membrane protein. the ami ... | 1995 | 7487497 |
| serological relationship between a donkey alphaherpesvirus (isolate m7/91) and equid herpesvirus type 1 and 4. | rabbit hyperimmune serum prepared against a donkey alphaherpesvirus isolate (m7/91), and against ehv-1 and ehv-4 was used to characterise the antigenic relationship between these 3 viruses. serum from immunised rabbits was always more specific for homologous virus and showed different cross reactivity for heterologous virus. it was concluded that the immunologic relationship between the m7/91 isolate and ehv-1, was closer than that between this isolate and ehv-4. a serological survey of donkeys ... | 1994 | 7776336 |
| prevalence of equine herpesvirus-1 and equine herpesvirus-4 infections in equidae species in turkey as determined by elisa and multiplex nested pcr. | in this report we examined the presence of specific antibodies against equine herpesvirus type 1 (ehv-1), and equine herpesvirus type 4 (ehv-4) in several equidae, including mules, donkeys, horses. the presence of ehv-1 and ehv-4 in respiratory diseases of equids, and ability of multiplex nested polymerase chain reaction (pcr) screening in simultaneous diagnosis of horses acutely infected by ehv-1 and ehv-4 were also investigated. sera from 504 horses, mules and donkeys sampled were tested for t ... | 2009 | 18649902 |
| surveillance programme for important equine infectious respiratory pathogens in the usa. | the prevalence and epidemiology of important viral (equine influenza virus [eiv], equine herpesvirus type 1 [ehv-1] and ehv-4) and bacterial (streptococcus equi subspecies equi) respiratory pathogens shed by horses presented to equine veterinarians with upper respiratory tract signs and/or acute febrile neurological disease were studied. veterinarians from throughout the usa were enrolled in a surveillance programme and were asked to collect blood and nasal secretions from equine cases with acut ... | 2011 | 21676986 |
| amiata donkey milk chain: animal health evaluation and milk quality. | this study presents an investigation of amiata donkey health and quality of milk for human consumption. thirty-one lactating dairy jennies were examined. the following samples were collected: faecal samples from the rectum of animals for parasitological examination; cervical swabs for the detection of bacteria causing reproductive disorders; and blood samples for serological diagnosis of main zoonotic (brucella spp., leptospira spp.) and donkey abortion agents (brucella spp., leptospira spp., sa ... | 2016 | 27853717 |
| equine herpes virus type 1 (ehv-1) and 4 (ehv-4) infections in horses and donkeys in northeastern turkey. | the herpesviruses infections in equides are caused by five different serotypes of viruses, belonging to family herpesviridae. the goal of this study was to conduct a seroepidemiological investigation of equine herpesvirus type 1 (ehv-1) and type 4 (ehv-4) in horses and donkeys raised in two provinces and their villages in northeastern turkey. a total of 666 samples from 423 horses and 243 donkeys that were not immunized against these infections were tested with elisa. while 52.48% of tested hors ... | 2015 | 27175200 |
| equine herpesvirus-1 myeloencephalopathy, an emerging threat of working equids in ethiopia. | although equine herpesvirus myeloencephalopathy (ehm) is a sporadic and relatively uncommon manifestation of equine herpesvirus-1 (ehv-1), it has the potential for causing devastating outbreaks in horses. up till now, there were no reported ehm outbreaks in donkeys and mules. this study describes the isolation and molecular characterization of ehv-1 from clinically ehm-affected horses (n = 6), mules (n = 3) and donkeys (n = 82) in ethiopia during outbreaks from may 2011 to december 2013. the inc ... | 2017 | 26010868 |
| development of a single-tube duplex evagreen real-time pcr for the detection and identification of ehv-1 and ehv-4. | the objective of this study was to develop a novel evagreen (eg) based real-time pcr technique for the simultaneous detection of equine herpesvirus 1 (ehv-1) and equine herpesvirus 4 (ehv-4) genomes from equine nasal swabs. viral genomes were identified based on their specific melting temperatures (t m), which are 88.0 and 84.4 °c for ehv-1 and ehv-4, respectively. the detection limitation of this method was 50 copies/μl or 0.15 pg/μl for ehv-1 and 5 copies/μl or 2.5 fg/μl for ehv-4. this assay ... | 2014 | 24615388 |