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igg antibody reactivity with trypanosoma cruzi and leishmania antigens in sera of patients with chagas' disease and leishmaniasis.sera from 38 paraguayans with positive skin tests to leishmania braziliensis panamensis and 51 sera from paraguayan patients in different stages of chagas' disease were analyzed by western blotting using antigen from trypanosoma cruzi y strain epimastigotes and from l. b. panamensis promastigotes. using a single serum sample, distinctive patterns of igg antibody response to both antigens were identified allowing the differentiation between t. cruzi infection, leishmania infection, and probable d ...19902125177
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