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human cutaneous leishmaniasis in ecuador: identification of parasites by enzyme electrophoresis.twenty-six strains of leishmania were isolated from cutaneous lesions in humans in 3 different geographical areas of ecuador. the species were identified by enzyme electrophoresis as leishmania braziliensis, l. panamensis, l. guyanensis, l. mexicana, and l. amazonensis.19902339754
natural infections with leishmania promastigotes in lutzomyia ayacuchensis (diptera: psychodidae) in an andean focus of ecuador.in the andean town of paute, ecuador, 2 of 97 (2%) lutzomyia ayacuchensis cáceres and bianchi were found to be naturally infected with leishmania promastigotes. the parasites were confined to the midgut of the sand fly, indicating they did not belong to the subgenus leishmania (viannia).19902388245
evidence for hybridization by multilocus enzyme electrophoresis and random amplified polymorphic dna between leishmania braziliensis and leishmania panamensis/guyanensis in ecuador.the taxonomic attribution of four leishmania stocks isolated from humans in ecuador has been explored by both multilocus enzyme electrophoresis and random amplified polymorphic dna. for three loci, mlee results showed patterns suggesting a heterozygous state for a diploid organism, while the corresponding homozygous states are characteristic of the leishmania panamensis/guyanensis complex and leishmania braziliensis, respectively. other enzyme loci showed characters attributable to either the l. ...19979304809
pcr detection and identification of leishmania parasites in clinical specimens in ecuador: a comparison with classical diagnostic methods.a simplified polymerase chain reaction (pcr)-based assay was used for detection and typing of leishmania parasites in clinical specimens from patients suspected of cutaneous leishmaniasis. using cultures as the reference standard, our pcr detection method was more sensitive (92%) than classical diagnostic techniques, including microscopy (42% sensitivity), histologic staining (33%), and igg enzyme-linked immunosorbent (20%). the pcr assay was also 100% specific. parasites in both lesion biopsies ...199910219292
genetic analysis of leishmania parasites in ecuador: are leishmania (viannia) panamensis and leishmania (v.) guyanensis distinct taxa?in the course of an epidemiologic survey in ecuador, the following collection of leishmania stocks was isolated: 28 from patients with clinical signs of leishmaniasis, 2 from sloths, 1 from a dog, and 4 from sand flies. for genetic characterization of these stocks, multilocus enzyme electrophoresis (mlee) and random amplified polymorphic dna (rapd) were used. twenty six of the 35 stocks were identified as either leishmania (v.) panamensis or l. (v.) guyanensis, 2 stocks were identified as l. (v. ...199910586922
usefulness of sampling with cotton swab for pcr-diagnosis of cutaneous leishmaniasis in the new world.in this study, we tested the polymerase chain reaction (pcr)-method to diagnose cutaneous leishmaniasis (cl) by taking exudate materials from lesions with cotton swabs, using our previously tested (pcr) panel comprised of leishmania (viannia) panamensis, l. (v.) braziliensis, l. (v.) guyanensis, l. (leishmania) mexicana and l. (l.) amazonensis. the objectives of the present study were to improve the sampling method convenient for the patients and to test the usefulness of samples taken with cott ...200211835896
identification of endotrypanum species from a sloth, a squirrel and lutzomyia sandflies in ecuador by pcr amplification and sequencing of the mini-exon gene.pcr amplification and nucleotide sequencing of the mini-exon gene revealed that four strains isolated from a sloth (choloepus hoffmanni), a squirrel (sciurus granatensis) and two sandflies (lutzomyia hartmanni) in ecuador were indistinguishable from endotrypanum monterogeii. another strain isolated from lu. hartmanni showed the high sequence similarity to e. schaudinni. since three of these strains have been previously identified as leishmania (viannia) equatorensis, the results demonstrate that ...200312808222
epidemiology of leishmaniasis in ecuador: current status of knowledge -- a review.although leishmaniasis is regarded as a significant health problem in ecuador by the ministry of health, and the incidence has increased over the last years, an official map on the geographic distribution of disease and sand fly vectors or a control strategy do not exist yet. this article reviews the current situation based on published information to improve our knowledge and understand the epidemiological situation of leishmaniasis in ecuador in order to help future research and to develop a n ...200415654419
atypical clinical variants in new world cutaneous leishmaniasis: disseminated, erysipeloid, and recidiva cutis due to leishmania (v.) panamensis.in recent times, there has been an increase in the number of reports for new and rare variants of cutaneous leishmaniasis (cl). here, we describe three unusual clinical forms of cl identified in ecuadorian children. a total of 131 patients with cl were diagnosed over a 2-year period of active search. in 3 (2.29%), the lesions were very unusual; these included erysipeloid, recidiva cutis (lrc), and disseminated leishmaniasis (dl). the erysipeloid case is characterized by erythematous and indurate ...200516103590
leishmaniasis recidiva cutis due to leishmania (viannia) panamensis in subtropical ecuador: isoenzymatic characterization.information regarding leishmaniasis recidiva cutis (lrc), a clinical variant of cutaneous leishmaniasis, in the new world is scarce. lrc is characterized by slowly progressing lesion(s) that appear after a variable period of time, from months to years, in or around the scar of an apparently clinically healed sore.200616445499
natural infection of lutzomyia tortura with leishmania (viannia) naiffi in an amazonian area of ecuador.natural infection of sand flies with leishmania parasites was surveyed in an amazonian area in ecuador where leishmaniasis is endemic. seventy-one female sand flies were dissected and one was positive for leishmania protozoa. the species of this sand fly was identified as lutzomyia (lu.) tortura on the basis of morphologic characteristics. analysis of the cytochrome b gene sequence identified the parasite as l. (viannia) naiffi. we report the distribution of l. (v.) naiffi in ecuador and detecti ...200818784239
a battery of 12 microsatellite markers for genetic analysis of the leishmania (viannia) guyanensis complex.we used 12 microsatellite markers developed for leishmania braziliensis to genotype 28 strains of the main species of the leishmania guyanensis complex (i.e. l. guyanensis and l. panamensis) collected in ecuador and peru. the important heterozygote deficits observed in these populations are similar with the previous data obtained in l. braziliensis and raise again the debate on the reproductive mode of these protozoan parasites. the data showed genetic polymorphism and geographical differentiati ...201020609264
imported new world cutaneous leishmaniasis in a traveller from slovakia.we present a case of imported leishmaniasis in a 31-year-old woman from slovakia, who visited the countries of south america for three months in 2011. on 29 and 31 august 2011, she was probably infected with leishmania parasites in the jungles of ecuador. approximately one week after returning to slovakia, a small papules appeared on patient's left leg. another wound was found after two weeks. both ulcers were enlarging. we proved amastigote forms of leishmania spp. only in repeated dermal scrap ...201525869571
an analysis of reported cases of leishmaniasis in the southern ecuadorian amazon region, 1986-2012.an analysis of reported cases of cutaneous leishmaniasis (cl) was performed using the data registered in the southern ecuadorian amazon region during 27 years from 1986 to 2012. the cases/subjects with both the suspected cl lesions and the amastigote-positive results were recruited for the analysis. the yearly occurrence of cases showed a markedly higher number during the six years, 1988 and 1993. after 1994 when the insecticide spraying campaign using helicopter in 1993-1994, the number dropped ...201525796313
genetic divergence in populations of lutzomyia ayacuchensis, a vector of andean-type cutaneous leishmaniasis, in ecuador and peru.haplotype and gene network analyses were performed on mitochondrial cytochrome oxidase i and cytochrome b gene sequences of lutzomyia (lu.) ayacuchensis populations from andean areas of ecuador and southern peru where the sand fly species transmit leishmania (leishmania) mexicana and leishmania (viannia) peruviana, respectively, and populations from the northern peruvian andes, for which transmission of leishmania by lu. ayacuchensis has not been reported. the haplotype analyses showed higher in ...201525312337
first human cases of leishmania (viannia) naiffi infection in ecuador and identification of its suspected vector species.epidemiological surveillance of leishmaniasis was conducted in a northern amazonian region of ecuador, in which cutaneous leishmaniasis cases were recently reported. sand flies were captured in the military training camp, and the natural infection of sand flies by leishmania species was examined. out of 334 female sand flies dissected, the natural infection by flagellates was microscopically detected in 3.9% of lutzomyia yuilli yuilli and 3.7% of lutzomyia tortura, and the parasite species were ...201324044975
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