development of a novel hand-held immunoassay for the detection of enterohemorrhagic escherichia coli o157:h7.escherichia coli o157:h7 is an important human pathogen responsible for numerous outbreaks of hemorrhagic colitis and hemolytic uremic syndrome world-wide. a portable detection device is needed for field testing and point-of-care testing. poly(dimethylsiloxane) (pdms) is a solid substrate well suited for adsorption of macromolecules. the purpose of this study was to develop a hand-held enzyme-linked immunosorbent assay (elisa) for the detection of e. coli o157:h7 antigens. the prototype consiste ...200415320634
antibacterial peptides derived from caprine whey proteins, by digestion with human gastrointestinal juice.peptides in caprine whey were identified after in vitro digestion with human gastrointestinal enzymes in order to determine their antibacterial effect. the digestion was performed in two continuing steps using human gastric juice (ph 2·5) and human duodenal juice (ph 8) at 37°c. after digestion the hydrolysate was fractionated and 106 peptides were identified. from these results, twenty-two peptides, located in the protein molecules, were synthesised and antibacterial activity examined. strong a ...201121554806
effect of pepsin-treated bovine and goat caseinomacropeptide on escherichia coli and lactobacillus rhamnosus in acidic conditions.caseinomacropeptide (cmp) is a 7-kda phosphoglycopolypeptide released from κ-casein during milk digestion and in the cheesemaking process. the objective of the study was to analyze the effect of pepsin-treated cmp from cow and goat milk on the resistance of escherichia coli and lactobacillus rhamnosus during acid stress. bacterial cells in the exponential growth phase were suspended in acidified phosphate buffered saline with or without pepsin-treated cmp. viability was determined during a 90-mi ...201222192178
development of a fermented goats' milk containing lactobacillus rhamnosus: in vivo study of health benefits.background: lactobacillus rhamnosus crl1505, a strain of goats' milk origin, is able to stimulate mucosal immunity and protect immunocompetent mice from intestinal and respiratory infections. results: in this work we developed and characterized a fermented goats' milk containing l. rhamnosus crl1505, and we demonstrated in a model of immunosuppression in mice that the final dairy product preserves the immunomodulatory properties of the strain. l. rhamnosus crl1505 survived the manufacturing proc ...201121604277
immunomodulatory activity of lactobacillus rhamnosus strains isolated from goat milk: impact on intestinal and respiratory infections.the immune stimulation induced by lactobacillus rhamnosus crl1505 (lr05) and l. rhamnosus crl1506 (lr06) on the resistance to infection with an intestinal pathogen (salmonella typhimurium) and a respiratory pathogen (streptococcus pneumoniae) was studied in swiss-albine mice experimental models. the cytokine profiles that induced the innate and specific immune response in both infectious processes were investigated. both strains were able to improve resistance against the intestinal pathogen. on ...201020395002
goat whey fermentation by kluyveromyces marxianus and lactobacillus rhamnosus release tryptophan and tryptophan-lactokinin from a cryptic zone of alpha-lactalbumin.angiotensin-i-converting enzyme (ace) inhibitors peptides were produced from unsupplemented acid goat whey fermented aerobically for 168 h by kluyveromyces marxianus and lactobacillus rhamnosus. this yeast-lactobacillus association is gras. two novel lactokinins were identified: nyw and w with ic50 of 20 and 0.86 mum respectively. they both were resistant toward simulated gastrointestinal digestion. in addition, wlahk was found in the hydrolysate. these three sequences belong to f(99-110) of alp ...200919650957
crude goat whey fermentation by kluyveromyces marxianus and lactobacillus rhamnosus: contribution to proteolysis and ace inhibitory activity.unsupplemented acid goat whey containing 0.96% protein and 2.76% lactose was fermented aerobically with 32 microflora extracted from various raw milk cheeses and dairy products. these microflora were screened for their ability to hydrolyse whey proteins (alpha-lactalbumin and/or beta-lactoglobulin) and to generate peptides inhibitors of angiotensin i converting enzyme. five microflora were able to degrade whey protein. the most efficient microflora was able to fully hydrolyse alpha-lactalbumin a ...200919121243
in vitro studies of the digestion of caprine whey proteins by human gastric and duodenal juice and the effects on selected microorganisms.the in vitro digestion of caprine whey proteins was investigated by a two-step degradation assay, using human gastric juice (hgj) at ph 2.5 and human duodenal juice (hdj) at ph 7.5. different protein and peptide profiles were observed after the first (hgj) and second (hdj) enzymatic degradation. the minor whey proteins serum albumin, lactoferrin and ig were rapidly degraded by hgj, while alpha-lactalbumin (alpha-la) and beta-lactoglobulin (beta-lg) were more resistant and survived both 30 and 45 ...200616925863
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