identification of a bisegmented double-stranded rna virus (picobirnavirus) in calf determine the incidence of rotavirus infection among dairy herds in the state of são paulo, brazil, 576 faecal samples obtained from calves aged 1-45 days with and without diarrhoea, reared on 63 dairy cattle farms, were analyzed. polyacrylamide gel electrophoresis (page) identified 28 samples positive for group a rotavirus, while four samples, two diarrhoeic and two non-diarrhoeic, showed a bisegmented genome with a typical picobirnavirus pattern. electron microscopy revealed spherical virus ...200312902184
serological and genomic characterization of porcine rotaviruses in thailand: detection of a g10 porcine rotavirus.a total of 557 fecal specimens collected from piglets with diarrhea in thailand were examined for rotavirus rna by polyacrylamide gel electrophoresis. twenty-three, one, and two samples were positive for group a, group b, and group c rotaviruses, respectively. two samples exhibited two segments found in picobirnavirus rna. rna electropherotyping of 23 group a rotaviruses showed that they were classified into five patterns. by serotyping by enzyme-linked immunosorbent assay and pcr, viruses in 3 ...19968727874
[viral gastroenteritis in domestic animals and zoonoses].etiologic investigations of infectious diarrhea were long limited to bacteria and protozoa. the advent of electron microscopy and molecular biology showed that diarrhea could also be caused by viruses, both in humans and in other animals. in 1969, electron microcopy was used to show, for the first time, the responsibility of a virus in a case of calf diarrhea. this "reo-like virus "was subsequently identified as a rotavirus, and was shown only four years later to be responsible for severe diarrh ...201022046708
molecular characterization of full-length genomic segment 2 of a bovine picobirnavirus (pbv) strain: evidence for high genetic diversity with genogroup i pbvs.we report here the molecular characterization of a bovine genogroup i picobirnavirus strain rubv-p detected from a 1-month-old diarrhoeic calf in eastern india. sequence comparisons and phylogenetic analysis of a short stretch of gene segment 2 of rubv-p revealed low nucleotide identities (51.2-64.9%) with and distant genetic relatedness to other genogroup i picobirnaviruses. the complete gene segment 2 sequence of rubv-p was obtained by the single primer amplification method with modifications. ...200919587136
picobirnavirus detection in bovine and buffalo calves from foothills of himalaya and central india.the present study describes detection of picobirnavirus (pbv) in faecal samples from bovine and buffalo calves employing the polyacrylamide gel electrophoresis (page). a total of 136 faecal samples from buffalo (nā€‰=ā€‰122) and cow calves (nā€‰=ā€‰14) exhibiting clinical signs of diarrhoea and from healthy calves were collected during 2007-2010 from subtropical (central india) and tarai area of western temperate himalayan foothills (uttarakhand). the dsrna nature of the virus was confirmed by nuclease ...201121479844
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