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cultivation of leishmania sp. in nutrient broth.bone-marrow aspiration and biopsy material samples obtained from two patients, one diagnosed as visceral and other as cutaneous leishmaniasis, were inoculated in novy, mcneal, nicolle (nnn) medium and nutrient broth (nb), containing fetal calf serum (fcs), penicillin and streptomycine. both media were incubated at 27 degrees c for 10 days and observed daily for l. infantum and l. major promastigotes. promastigotes were observed in nutrient broth after the first day, while in nnn media after the ...19957665939
acquisition of iron from transferrin and lactoferrin by the protozoan leishmania chagasi.leishmania chagasi, the cause of south american visceral leishmaniasis, requires iron for its growth. however, the extent to which different iron sources can be utilized by the parasite is not known. to address this question, we studied acquisition of iron from lactoferrin and transferrin by the extracellular promastigote form of l. chagasi during growth in vitro. a promastigote growth medium based on minimal essential medium supplemented with iron-depleted serum supported promastigote growth on ...19948039896
[coxiella burnetii: what is the reality?].after the second world war, in italy q fever or coxiellosis has been shown a significant relevance, a recrudescence with an epidemic state for over ten years. later, the infectious disease occurred as endemic since the 80s, the outbreaks were just isolated. workflows analysis of some authors has demonstrated the spread out of the infection throughout italian herds with a prevalence ranging from 1.2 per cent to 10 per cent. our survey carried out throughout campania area in cattle has shown a rea ...200415305702
risk factors for canine neosporosis in farm and kennel dogs in southern italy.neosporosis by neospora caninum causes losses to livestock production through abortion in cattle while, in dogs, it induces neuromuscolar disease. this study investigated neosporosis seroprevalence associated risk factors (including the seropositivity to leishmaniosis) in dogs of southern italy, determined the prevalence of n. caninum oocyst shedding and examined the relationship between seroprevalence of neosporosis in farm dogs and cattle. using an inhibition elisa, 20.9% of 306 dogs had perce ...200717257762
kennel dogs as sentinels of leishmania infantum, toxoplasma gondii, and neospora caninum in majorca island, spain.kennel dogs can serve as sentinels and/or reservoirs of diseases of veterinary and zoonotic interest because they have often roamed free and lived outdoors, thus being exposed to pathogens. we tested dogs from the kennel of inca (majorca/mallorca, balearic islands, spain) for evidence of infection with three protozoan parasites: leishmania infantum, toxoplasma gondii, and neospora caninum. exposure to l. infantum was found in 56.3% of 48 dogs (37.5% by western blot, 43.8% by pcr). only 30% of in ...201020689966
nuclear dna polymerase beta from leishmania infantum. cloning, molecular analysis and developmental regulation.we have identified a novel polymerase beta (pol beta)-like enzyme from leishmania infantum, a parasite protozoon causing disease in humans. this protein, named li pol beta, shows a nuclear localization that contrasts with the mitochondrial localization of pol beta from crithidia fasciculata, a closely related parasite, the only polymerase beta described so far in trypanosomatidae. li pol beta, that belongs to the dna polymerase x family, displays an evolutionarily conserved pol beta-type dna pol ...200111557814
cl gene cluster encoding several small nucleolar rnas: a comparison amongst trypanosomatids.small nucleolar rnas (snornas) are small non-coding rnas that modify rna molecules such as rrna and snrna by guiding 2'-o-ribose methylation (c/d box snorna family) and pseudouridylation reactions (h/aca snorna family). h/aca snornas are also involved in trans-splicing in trypanosomatids. the aims of this work were to characterise the cl gene cluster that encodes several snornas in trypanosoma rangeli and compare it with clusters from trypanosoma cruzi, trypanosoma brucei, leishmania major, leis ...200919547875
cutaneous leishmaniasis caused by leishmania infantum transmitted by phlebotomus tobbi.transmission of cutaneous leishmaniasis (cl) caused by leishmania infantum was studied in south anatolia, turkey. small, non-ulcerating lesions prevailed and patients were negative in rk39 tests for antibody detection for human visceral leishmaniasis (vl). the most abundant sand fly species, phlebotomus tobbi, was found positive for leishmania promastigotes with a prevalence of 1.4% (13 out of 898 dissected females). the isolated strains were identical with those obtained from patients with cl a ...200918761342
crude ethanolic extract, lignoid fraction and yangambin from ocotea duckei (lauraceae) show antileishmanial activity.crude ethanolic extract, lignoid fraction and the purified compound yangambin were obtained from ocotea duckei (lauraceae) and their antileishmanial activity was tested against promastigote forms of leishmania chagasi and leishmania amazonensis cultivated in schneider medium, supplemented with 20% of fetal bovine serum. all substances presented antileishmanial activity with ic50 values of 135.7 microg/ml for the crude ethanolic extract, 26.5 microg/ml for the lignoid fraction and 49.0 microg/ml ...200717708438
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