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identification and characterization of is1296 in mycoplasma mycoides subsp. mycoides sc and presence in related mycoplasmas.is1296, a new insertion sequence belonging to the is3 family of insertion elements has been identified in mycoplasma mycoides subsp. mycoides (mmm) biotype small colony (sc), the agent of contagious bovine pleuropneumonia (cbpp). is1296 is 1485-bp long and has 30-bp inverted repeats. it contains two open reading frames, orfa and orfb, which show significant similarities to the orfs which encode the transposase function of is elements of the is3 family, in particular is150 of escherichia coli. is ...19957628725
genetic and serological analysis of the immunogenic 67-kda lipoprotein of mycoplasma sp. bovine group 7.the gene encoding a lipoprotein of 67 kda, named p67, was cloned from mycoplasma sp. bovine group 7 strain pg50 and expressed in escherichia coli k12. analysis of the amino acid sequence derived from the dna sequence of the p67 gene revealed a typical prokaryotic signal peptidase ii membrane lipoprotein lipid attachment site and a transmembrane structure domain in the leader sequence at the amino-terminal end of the protein. protein p67 showed 91% identical amino acid residues to the lipoprotein ...19989766210
antigen heterogeneity among mycoplasma mycoides subsp. mycoides sc isolates: discrimination of major surface proteins.the protein and antigen profiles of 60 isolates, strains and the type strain pg1 of mycoplasma mycoides subsp. mycoides s.c. were compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblot analysis. analysis using contagious bovine pleuropneumonia antisera and hyperimmune rabbit sera against several representative strains revealed some differences in protein profiles and variability in antigens among strains from different geographic regions. the most common antigenic ...19989810618
genomic and antigenic differences between the european and african/australian clusters of mycoplasma mycoides subsp. mycoides sc.mycoplasma mycoides subsp. mycoides small-colony type (sc), the aetiological agent of contagious bovine pleuropneumonia (cbpp), can be grouped into two major, epidemiologically distinct, clusters. one cluster contains strains isolated from different european countries since 1980 and a second cluster contains african and australian strains collected over the last 50 years. genetic analysis of representative strains from the two clusters revealed a genomic segment of 8.84 kb, located close to a co ...200010708386
phylogeny of the mycoplasma mycoides cluster as shown by sequencing of a putative membrane protein gene.the mycoplasma mycoides cluster is made of six species that are closely related both genetically and phenotypically. two are of particular importance, m. mycoides subsp. mycoides sc causing contagious bovine pleuropneumonia and m. capricolum subsp. capripneumoniae causing contagious caprine pleuropneumonia. the sequences of a putative membrane protein gene and partial flanking open reading frames have been obtained from various strains in this cluster, including all reference strains. sequence a ...200010727835
rapid detection of contagious bovine pleuropneumonia by a mycoplasma mycoides subsp. mycoides sc capsular polysaccharide-specific antigen detection latex agglutination test.a latex agglutination test (lat) has been developed for the diagnosis of contagious bovine pleuropneumonia (cbpp). the latex microspheres were coated with mmmsc polyclonal immunoglobulin g antiserum and detected mmmsc antigen in the serum of cattle infected with cbpp and in growth medium containing mmmsc. the specific antigen recognized by this test appeared to be the capsular polysaccharide (cps). the lat recognized all 23 strains of mmmsc examined in this study, with a sensitivity level of 2 n ...200312626448
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