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genetic characterization of a phospholipase c gene from candida albicans: presence of homologous sequences in candida species other than candida albicans.phospholipase c (plc) enzymes are essential in regulating several important cellular functions in eukaryotes, including yeasts. in this study, pcr was used to identify a gene encoding plc activity in candida albicans, using oligonucleotide primers complementary to sequences encoding highly conserved amino acid regions within the x domains of previously characterized eukaryotic phospholipase c genes. the nucleotide sequence of the c. albicans gene, caplc1 (2997 bp), was determined from a recombin ...19989467900
candidiasis caused by candida glabrata in the forestomachs of a calf.a female japanese black calf suffering from diarrhoea was treated with six different antibiotics for 9 days. the diarrhoea worsened and the calf was killed when 17 days old. at necropsy, the mucosal surface of the forestomachs showed diffuse haemorrhagic necrosis and pseudomembrane formation. candida glabrata infection was diagnosed histologically, immunohistochemically, and by isolation and examination of the yeast. the intensive antibiotic treatment was considered to have been a predisposing o ...19947836573
effects of bovine parainfluenza-3 virus on phagocytosis and phagosome-lysosome fusion of cultured bovine alveolar macrophages.bovine parainfluenza-3 virus (pi-3v) replicated in cultured bovine alveolar macrophages (bam) in vitro. cytopathic changes included spindle cell and giant cell formation, diffuse cell lysis, and cellular detachment progressing to total destruction of the monolayer. direct immunofluorescent microscopy revealed viral antigen in greater than 90% of bam on post-inoculation day (pid) 3. virus titers in culture supernatants increased by a factor of 1.6 x 10(6) tcid50 by pid 6. candida glabrata was pha ...19836314852
yeasts in the gastrointestinal tract of preweaned calves and possible involvement of candida glabrata in neonatal calf diarrhea.to examine the possibility of a mycotic involvement in neonatal calf diarrhea (ncd) the presence of fungi was assessed in (a) the intestinal contents of dead calves and fecal samples submitted for routine laboratory examination, (b) fecal specimens, sampled once in winter and once in summer, of calves raised on 2 farms with different management systems, and (c) mucosal scrapings of various segments of the digestive tract of a diarrheic calf, massively shedding candida glabrata. c. glabrata was t ...19989725026
the contribution of electrostatic forces to the process of adherence of candida albicans yeast cells to substrates.the contribution of electrostatic interactions to the adherence process of candida albicans and other candida species was investigated by mixing cationic or anionic exchange resins possessing free -coo- or -nh+4 groups, respectively, on their surface. the adherence process of yeast cells to the anionic exchange resin is a saturable event that is essentially complete by 60 min. there is no measurable interaction of yeast cells with the cationic exchange resin. all clinical isolates of c. albicans ...19948076801
fungal infection of the radicular dentin.although fungi have been detected in infected root canals, their precise role as endodontic pathogens has not yet been elucidated. the purpose of this study was to investigate the pattern of radicular dentin colonization by five fungal species. bovine root sections were infected with each of the following fungal species: candida albicans, candida glabrata, candida guilliermondii, candida parapsilosis, and saccharomyces cerevisiae. after 14 days, the sections were fixed in glutaraldehyde, split i ...200212470022
sterol uptake in candida glabrata: rescue of sterol auxotrophic strains.candida glabrata is emerging as a more common and important human pathogen. it is less susceptible to azole antifungals than candida albicans, thus, posing some unique treatment challenges. previously undetected c. glabrata isolates were identified from clinical specimens by adding bile to the growth medium. cholesterol was found to be the responsible ingredient in bile. six bile-dependent isolates were characterized and were found to exhibit wild-type equivalent growth when provided human or bo ...200515893902
influence of diet on the shedding of candida glabrata by experimentally infected preweaned calves.the influence of three diets, comprising dam's milk (dm) from the same farm, commercial milk replacer with (cmrl) or without 3.2% lactose (cmr), on the duration and intensity of candida glabrata shedding in the faeces of preweaned calves following experimental oral infection was examined. shedding of other potential enteric pathogens was also monitored. the duration and intensity of c. glabrata shedding in dm-fed calves were reduced significantly compared with the calves fed the two diets based ...200212505403
yarrowia lipolytica vesicle-mediated protein transport pathways.protein secretion is a universal cellular process involving vesicles which bud and fuse between organelles to bring proteins to their final destination. vesicle budding is mediated by protein coats; vesicle targeting and fusion depend on rab gtpase, tethering factors and snare complexes. the génolevures ii sequencing project made available entire genome sequences of four hemiascomycetous yeasts, yarrowia lipolytica, debaryomyces hansenii, kluyveromyces lactis and candida glabrata. y. lipolytica ...200717997821
identification of candida species isolated from bovine mastitic milk and their in vitro hemolytic activity in western turkey.in this study, identification of 207 candida isolates, previously isolated from mastitic bovine quarter milk samples at the level of genus, was made using api 20 c aux system. the most frequently isolated species were candida krusei (34.8%), followed by candida rugosa (16.4%), candida kefyr (12.6%), candida albicans (10.1%), and candida tropicalis (9.2%). less common isolates were candida zeylanoides (5.8%), candida parapsilosis (4.3%), candida guilliermondii (3.4%), candida famata (1.9%), and c ...201019921462
characterization of a parainfluenza virus isolated from a bottlenose dolphin (tursiops truncatus).a novel member of the parainfluenza virus family was identified in a bottlenose dolphin with respiratory disease. the case animal was a 19-year old male atlantic bottlenose dolphin (tursiops truncatus) that presented with signs of respiratory illness, including raspy, foul-odored breaths and cream-colored exudate from the blowhole. focally extensive pyogranulomatous bronchointerstitial pneumonia with moderate numbers of intralesional yeast organisms was identified on histopathological examinatio ...200818031960
yeasts isolation from bovine mammary glands under different mastitis status in the mexican high plateu.the mastitis subclinical and clinical in cows caused by fungi has been increased specially by yeast of the genus candida.201121349344
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