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enterovirus replication in porcine ileal explants.organ explants of porcine ileum were cultured in different media for up to 48 h. tissue preservation was evaluated by light microscopy and by transmission and scanning electron microscopy. cellular structure was well maintained after incubation for 48 h in cmrl-1066 supplemented with insulin and cortisone. explants of absorptive or lymphoid tissue from young or adult pigs were incubated with either coxsackievirus b5 (which is infectious for swine) or human poliovirus type 1 (which served as a co ...19872821187
coxsackievirus-cell interactions that initiate infection in porcine ileal explants.coxsackievirus b5 (cb5) labeled with tritiated uridine was used to trace the interaction of the virus with explant cultures of porcine ileum. similarly labeled human poliovirus 1 (po 1), which is not specifically retained by porcine tissue, was used as a control. the explant procedure employed could maintain ileal tissue in a differentiated state for up to 48 hours. porcine ileum was acquired from both young (4-6 week-old) and adult (9-11 month-old) animals. inoculated explants of either absorpt ...19882843148
complete nucleotide sequence of a coxsackie b5 virus and its relationship to swine vesicular disease virus.we report the first complete nucleotide sequence of the picornavirus coxsackievirus b5 (cb5), strain 1954/uk/85, an isolate from a case of hand-foot-and-mouth disease. we have compared the sequence with those of other coxsackie b viruses, coxsackievirus a9, poliovirus and swine vesicular disease virus (svdv). the genes encoding the three major capsid proteins are most closely related to those of svdv but the 5' and 3' noncoding regions and the p3 gene are more similar to the corresponding region ...19938388019
genotypic variation in coxsackievirus b5 isolates from three different outbreaks in the united states.genomic sequences in vp1/2a and 5'-non-coding region of 10 isolates of coxsackievirus b5 from three outbreaks were compared with published sequences of another coxsackievirus b5, swine vesicular disease virus, coxsackievirus b1, coxsackievirus b3, and coxsackievirus b4. isolates of coxsackievirus b5 from the same outbreak showed close relations, not exceeding 7.2% in nucleotide differences. differences were greater between isolates from different outbreaks, varying between 8.4 and 16%. we have a ...19958578854
swine vesicular disease: an overview.swine vesicular disease (svd) is a notifiable viral disease of pigs included on the office international des epizooties list a. the first outbreak of the disease was recognized in italy in 1966. subsequently, the disease has been reported in many european and asian countries. the causative agent of the disease is svd virus which is currently classified as a porcine variant of human coxsackievirus b5 and a member of the genus enterovirus in the family picornaviridae. from a clinical point of view ...200011061956
isolation of coxsackievirus b5 from pigs.a cytopathic virus was isolated from young pigs suffering from severe diarrhea in okinawa, japan in 1986. the disease was highly contagious among young pigs. the physico-chemical properties of the virus indicated an enterovirus, but, no of serological relationship was detected with reference strains of porcine enteroviruses. with the aid of genbank for genomic sequence data, rt-pcr and hybridization method was performed. the viral isolate was identified as the coxsackievirus (cv) b5 of human ent ...200111497077
swine vesicular disease virus. pathology of the disease and molecular characteristics of the virion.swine vesicular disease is a highly contagious disease of pigs that is caused by an enterovirus of the family picornaviridae. the virus is a relatively recent derivative of the human coxsackievirus b5, with which it has high molecular and antigenic homology. the disease is not severe, and affected animals usually show moderate general weakening and slight weight loss that is recovered in few days, as well as vesicular lesions in the mucosa of the mouth and nose and in the interdigital spaces of ...200011708597
crystal structure of swine vesicular disease virus and implications for host adaptation.swine vesicular disease virus (svdv) is an enterovirus of the family picornaviridae that causes symptoms indistinguishable from those of foot-and-mouth disease virus. phylogenetic studies suggest that it is a recently evolved genetic sublineage of the important human pathogen coxsackievirus b5 (cbv5), and in agreement with this, it has been shown to utilize the coxsackie and adenovirus receptor (car) for cell entry. the 3.0-a crystal structure of strain uk/27/72 svdv (highly virulent) reveals th ...200312692248
sequence analysis of the 5' untranslated region of swine vesicular disease virus reveals block deletions between the end of the internal ribosomal entry site and the initiation codon.swine vesicular disease virus (svdv) is a picornavirus closely related to the human pathogen coxsackievirus b5. in common with other picornaviruses, the 5' untranslated region (5' utr) of svdv contains an internal ribosomal entry site (ires) that plays an important role in cap-independent translation. the aim of this study was to use rt-pcr and sequencing to characterize a fragment of the 5' utr encompassing the entire ires. sequence analysis demonstrated high nucleotide identities within the ir ...200516186229
comparison of proliferation and cytopathogenicity of swine vesicular virus and coxsackievirus b5.sequential appearance of both swine vesicular disease virus and coxsackievirus b5 antigens in a pig kidney cell line was studied by immunofluorescence and electron microscopy. the replication cycle of each virus was approximately 3-4 h. viral antigens were demonstrable in the cytoplasm 2 h after inoculation. a compact mass of fluorescence was seen when cells showed cytopathogenic effect at 5.5 h. after 3 h, a few viral particles, seen by electron microscopy, were in the cytoplasm. morphological ...19816269809
pathogenesis of coxsackievirus-b5 acquired from intra-renal porcine islet cell xenografts in diabetic mice.we previously demonstrated the ability of a human isolate of coxsackievirus-b5 (cvb5) to infect productively adult porcine islet cells (pics) in vitro. pics infected with cvb5 remain viable, and upon transplantation reversed diabetes in c56bl/6 mice for up to 5 days.200919392724
development of a real-time pcr assay based on primer-probe energy transfer for the detection of swine vesicular disease virus.a real-time pcr assay based on primer-probe energy transfer (priproet) was developed to detect swine vesicular disease virus (svdv). specificity tests of svdv and heterologous virus showed specific amplification of svdv strains only. the amplification plot for the closely related coxsackievirus b5 remained negative. the sensitivity of assay was five copies of viral genome equivalents. a key point of the assay is tolerance toward mutations in the probe region. melting curve analysis directly afte ...200616835700
mechanisms of beta cell death during restricted and unrestricted enterovirus infection.coxsackie b virus (cvb-5) infections potentially trigger and accelerate pancreatic beta cell damage leading to type 1 diabetes. in vivo, all viruses face natural resistance mediated by various host factors which restrict the progression of infection. thus, the aims of this study were to generate a tissue culture model of restricted coxsackie b virus infection in primary islet cells by preventing the production of viral progeny with a selective inhibitor of viral rna replication and to investigat ...200414748069
coxsackievirus b5 and the relationship to swine vesicular disease virus. 19979294928
replicase gene of coxsackievirus b3.a cdna copy covering two-thirds of the coxsackievirus b3 genome was cloned in the psti site of the pbr322 vector. a nucleotide sequence containing the gene for the viral replicase and the 3' noncoding region of the coxsackievirus b3 genome was determined. the predicted amino acid sequence of the coxsackievirus b3 replicase was shown to be remarkably similar to that of the poliovirus 1 replicase. the 3' noncoding region, in contrast, was only weakly homologous to the poliovirus 1 sequence but sho ...19846088796
molecular characterization of coxsackievirus b5 isolates.coxsackie b viruses of serotype 5 are associated frequently with sporadic cases of neurological diseases, epidemics of meningitis, and chronic diseases such as cardiomyopathy and diabetes. in this article, 15 strains of coxsackievirus b5 isolated from patients with neurological disorders and healthy people were investigated by partial sequencing in the 5' half of the vp1 region and compared to other published sequences of coxsackievirus b5, in the same genomic region. all coxsackievirus b5 seque ...200414695669
structure of swine vesicular disease virus: mapping of changes occurring during adaptation of human coxsackie b5 virus to infect swine.the structure of swine vesicular disease virus (svdv) was solved and refined at a 3.0-a resolution by x-ray crystallography to gain information about the role of sequence changes that occurred as this virus evolved from the parental human pathogen coxsackievirus b5 (cvb5). these amino acid substitutions can be clustered in five distinct regions: (i) the antigenic sites, (ii) the hydrophobic pocket of the vp1 beta-sandwich, (iii) the putative car binding site, (iv) the putative heparan sulfate bi ...200312941886
mapping of linear epitopes on the capsid proteins of swine vesicular disease virus using monoclonal antibodies.the antigenic linear map of swine vesicular disease virus (svdv) has been studied using a repertoire of monoclonal antibodies (mabs) raised against a recombinant svdv polyprotein, p1. peptide-scanning analyses, cross-reactivity studies with homologous and heterologous viruses and predicted location on a computer-generated three-dimensional model of the capsid proteins have allowed the identification of five main linear sites. two sites, the n terminus of vp3 and amino acids 51-60 on vp1, corresp ...200212029154
singleton reactors in the diagnosis of swine vesicular disease: the role of coxsackievirus b5.swine vesicular disease virus (svdv) and coxsackie b5 virus (cvb5) are closely related viruses that can infect swine and man and give rise to cross-reacting serum antibodies. it is, therefore, possible that svd antibodies found in serologic screenings of pigs are induced by cvb5. single positive animals found in screening programmes are generally referred to as singleton reactors (sr). to determine whether sr in svdv screenings are induced by cvb5 infection, virus neutralisation tests (vnts) and ...200010973703
molecular analysis of the prototype coxsackievirus b5 genome.to facilitate studies of the phylogenetic relationship between enteroviruses, in particular the prototype strain (faulkner) of coxsackievirus b5 (cvb5f) and other cvb5 isolates and to facilitate studies of the interactions between cvb5f and the target cell, the complete nucleotide sequence of the prototype has been determined. the complete sequence was collected from three overlapping reverse transcription polymerase chain reaction (rt-pcr) generated amplicons. molecular analysis of the cvb5f ge ...200010752549
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