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the molecular biology of the morbillivirus (measles) group.the morbilliviruses are a closely related group of important human and animal pathogens. the best known members of the group are measles virus in man and canine distemper virus in dogs. the group also includes two other serious animal diseases, rinderpest or cattle plague and peste des petits ruminants in sheep and goats. the latter viruses are of great economic importance in africa, asia and the middle east. persistence of these viruses in some form is a possible mechanism whereby life-long imm ...19873332767
peste des petits ruminants antibodies in east africa. 19957754596
peste des petits ruminants in ethiopian goats.an outbreak of disease characterised by fever, ocular and nasal discharge, coughing and sneezing, oral necrosis, diarrhoea, enteritis and pneumonia in goats was shown by the use of specific cdna probes to have been peste des petits ruminants, confirmed for the first time in ethiopia. both morbidity and mortality rates were high in goats but sheep were not affected.19947941031
serological evidence of the absence of peste des petits ruminants in tanzania. 200010819135
immune responses in goats to recombinant hemagglutinin-neuraminidase glycoprotein of peste des petits ruminants virus: identification of a t cell determinant.peste des petits ruminants virus (pprv), a member of the genus morbillivirus within the family paramyxoviridae, causes a fatal disease 'peste des petits ruminants' in goats and sheep. this enveloped virus is antigenically closely related to rinderpest virus (rpv), which causes a similar but distinct disease in large ruminants. pprv harbors two major surface glycoproteins, the hemagglutinin-neuraminidase (hn) and the fusion (f) proteins. the surface glycoproteins of morbilliviruses are highly imm ...200111535334
prevalence, distribution, and host range of peste des petits ruminants virus, turkey.peste des petits ruminants virus (pprv, genus morbillivirus), which causes a severe disease in sheep and goats, has only recently been officially declared to be present in turkey. we carried out a study to determine the prevalence, distribution, and host range of pprv in turkey. a total of 1,607 animals, reared in 18 different locations, were monitored for the presence of antibodies to pprv and the related virus of large ruminants, rinderpest virus (rpv). only two farms had animals that were fre ...200212095439
full genome sequence of peste des petits ruminants virus, a member of the morbillivirus genus.peste des petits ruminants virus (pprv) causes an acute febrile illness in small ruminant species, mostly sheep and goats. pprv is a member of the morbillivirus genus which includes measles, rinderpest (cattle plague), canine distemper, phocine distemper and the morbilliviruses found in whales, porpoises and dolphins. full length genome sequences for these morbilliviruses are available and reverse genetic rescue systems have been developed for the viruses of terrestrial mammals, with the excepti ...200515845262
monkey cv1 cell line expressing the sheep-goat slam protein: a highly sensitive cell line for the isolation of peste des petits ruminants virus from pathological specimens.peste des petits ruminants (ppr) is an important economically transboundary disease of sheep and goats caused by a virus which belongs to the genus morbillivirus. this genus, in the family paramyxoviridae, also includes the measles virus (mv), canine distemper virus (cdv), rinderpest virus (rpv), and marine mammal viruses. one of the main features of these viruses is the severe transient lymphopaenia and immunosuppression they induce in their respective hosts, thereby favouring secondary bacteri ...201121371505
immune responses to hemagglutinin-neuraminidase protein of peste des petits ruminants virus expressed in transgenic peanut plants in sheep.peste des petits ruminants (ppr) is an acute, highly contagious disease of small ruminants caused by a morbillivirus, peste des petits ruminants virus (pprv). the disease is prevalent in equatorial africa, the middle east, and the indian subcontinent. a live attenuated vaccine is in use in some of the countries and has been shown to provide protection for at least three years against ppr. however, the live attenuated vaccine is not robust in terms of thermotolerance. as a step towards developmen ...201021211855
[genome sequencing and analysis of a peste des petits ruminants virus isolate, china/tib/07].peste des petits ruminants virus is a member of morbillivirus paramyxoviridae. the complete genome of a peste des petits ruminants virus (pprv) isolate, china/tib/07 was sequenced and molecular characteristics was analyzed. the internal sequences of the virus genome were amplified by rt-pcr with primers designed according to the published data in genbank, while the sequences of the 3' and 5' ends of the genome were determined by race. amplification products were directly sequenced,assembled and ...201020836387
[sequence analysis of the matrix protein and fusion protein genes of a field peste des petits ruminants virus strain from tibet, china].the nucleotide sequences of m and f genes from a field strain of peste des petits ruminants virus (pprv) ("china/tib/gej/07-30") was firstly determined. the m gene was 1 483 nucleotides in length with a single open reading frame (orf), encoding a protein of 335 amino acids. the f gene was 2411 nucleotides in length, encoding a protein of 546 amino acids. the resulting nucleotide sequence and the deduced amino acid sequences were compared with the homologous regions of other pprv isolates. the nu ...201020836385
sequence analysis of morbillivirus cd150 receptor-signaling lymphocyte activation molecule (slam) of different animal species.signaling lymphocyte activation molecule-slam (cd150) molecule has been reported as a putative receptor for most morbilliviruses for their respective host species. in this study, we determined the complete nucleotide sequence of the gene coding for the morbillivirus receptor-slam from the four species, namely, goat (capra hircus), sheep (ovis aries), indian cattle (bos indicus), and buffalo (bubalus bubalis). the nucleotide (nt) open reading frame sequence of slam gene in all the four species st ...200919669672
peste des petitis ruminants morbillivirus infection in lambs and young goats at qassim region, saudi arabia.an outbreak of peste des petits ruminants (ppr) in lambs and young goats of najdi breed of sheep and goats occurred during winter 2005 at qassim region of central saudi arabia. the ppr infection was confirmed by demonstration of antibodies against the virus in the serum of clinically-ill young sheep and goats using competitive elisa test. clinical examination of infected animals showed fever, salivation, lacrimation, mucopurulent nasal discharge, difficult breathing and diarrhoea. the disease wa ...200918504645
development of one-step real-time rt-pcr assay for detection and quantitation of peste des petits ruminants virus.in this study, a rapid and specific taqman-based, one-step real-time quantitative reverse transcription pcr (qrt-pcr) has been described for the detection of peste des petits ruminants virus (pprv). primers and probe were designed based on the nucleocapsid protein gene sequence. the real-time qrt-pcr assay was able to detect pprv isolates from very distinct geographical areas (africa, middle east and asia). the specificity of the assay was assessed by including rinderpest virus and other morbill ...200818243345
natural peste des petits ruminants virus infection: novel pathologic findings resembling other morbillivirus infections.the present study describes pathologic and virologic findings in 15 sheep and 6 goats that died of natural peste des petits ruminants virus infection in turkey. pathologic findings included erosive-ulcerative stomatitis, fibrino-necrotic tracheitis, bronchointerstitial pneumonia, multifocal coagulation necroses in the liver, and severe lymphocytolysis in lymphoid tissues. syncytial cells were conspicuous, especially in the oral mucosa, pulmonary alveoli, liver, and lymphoid tissues. in addition ...200717606509
the threat of peste des petits ruminants: progress in vaccine development for disease control.peste des petits ruminants (ppr) is a highly contagious animal disease caused by a virus in the genus morbillivirus, family paramyxoviridae. this infection is responsible for high morbidity and mortality in sheep and goats and in some small wild ruminant species. the huge number of small ruminants, which are reared in the endemic areas makes ppr a serious disease threatening the livelihood of poor farmers. taking advantage of the closely relationship between rinderpest and ppr viruses, the atten ...200717399862
clinical and para-clinical findings of a recent outbreaks of peste des petits ruminants in iran.peste des petits ruminants (ppr) is a highly contagious and infectious viral disease of domestic and wild small ruminants characterized by fever, erosive stomatitis, conjunctivitis, gastroenteritis and pneumonia. goats are usually more severely affected than sheep. peste des petits ruminants is caused by a paramyxovirus of the morbillivirus genus. in march 2004, a flock of sheep in tehran province with 430 deaths was visited. according to the history taken from the owner, at disease onset most o ...200617123359
control of peste des petits ruminants: classical and new generation vaccines.peste des petits ruminants is a highly contagious disease of domestic and wild small ruminants. it seems to be the major constraint in the development of small ruminant production in areas where it is endemic. included in the list a of the international zoosanitary code, it is also part of the fao empress programme. classically, it is characterised by fever, nasal and ocular discharges, diarrhoea, respiratory distress, mucosal erosive lesions and death in 40-80% of acute cases. all these clinica ...200314677682
monoclonal antibody-based competitive elisa for simultaneous detection of rinderpest virus and peste des petits ruminants virus antibodies.an experimental competitive enzyme-linked immunosorbent assay (morbillivirus celisa) using a recombinant n antigen (rrpv n) expressed in a baculovirus and a ruminant morbillivirus (rpv and pprv)-specific monoclonal antibody (p-13a9) was developed for simultaneous detection of rinderpest virus (rpv) and peste des petits ruminants virus (pprv) antibodies and its diagnostic performance was evaluated. a set of known reference antisera against rpv and pprv belonging to different lineages, experimenta ...200314516703
economics of prophylaxis against peste des petits ruminants and gastrointestinal helminthosis in small ruminants in north cameroon.data on reproduction and mortality were collected over one year from 5100 sheep and 13,300 goats in treated and control flocks. the treated animals received vaccination against peste des petits ruminants (ppr) and anthelmintics twice a year. productivity parameters (fecundity and mortality rates) obtained with and without prophylaxis were fitted into a benefit-cost economic analysis model and run for project lifespans varying from one to five years. at a 7% discount rate, the overall benefits fo ...200011147279
improved technique for transient expression and negative strand virus rescue using fowlpox t7 recombinant virus in mammalian cells.the suitability of recombinant t7 polymerase produced using either the highly attenuated mva strain of vaccinia (mva-t7) or fowlpox virus (fp-t7) for transient expression and negative strand virus rescue was compared in two mammalian cell lines (mdbk and vero) and in primary cells of bovine, ovine and caprine origin. such primary cells are more permissive for the growth of wild type strains of morbilliviruses, such as rinderpest virus and peste des petits ruminants virus. mva-t7 was found to be ...200010996645
immunohistochemical detection of peste des petits ruminants viral antigen in formalin-fixed, paraffin-embedded tissues from cases of naturally occurring infection. 199910353363
the isolation of peste des petits ruminants virus from northern india.the aetiological agent responsible for an epizootic of a rinderpest-like disease afflicting sheep and goats in three states of northern india was confirmed as peste des petits ruminants virus. to differentiate the virus from rinderpest a number of diagnostic tests were used, including immunocapture elisa, specific oligonucleotide primers in a reverse transcriptase polymerase chain reaction, immunofluorescence with virus specific monoclonal antibodies and virus isolation. the virulence profile of ...19968870184
evaluation of polymerase chain reaction for the detection and characterisation of rinderpest and peste des petits ruminants viruses for epidemiological studies.the high sequence variability found in rna viruses makes it difficult to design primers for reverse transcription-polymerase chain reaction amplification which will be certain to work with all new field isolates. to overcome this problem for the detection and differential diagnosis of rinderpest (rp) and peste des petits ruminants (ppr) viruses (v), we have designed several sets of primers, based on well-conserved sequences in the p and f genes. analysis of a large number of field isolates from ...19958837881
protection of goats against peste des petits ruminants with a vaccinia virus double recombinant expressing the f and h genes of rinderpest virus.peste des petits ruminants (ppr) is a viral disease of goats and sheep characterized by necrotizing and erosive stomatitis, enteritis and pneumonia. the causative agent, pprv, is a member of the family paramyxoviridae and the genus morbillivirus. other members of the genus include rinderpest (rpv), measles, canine distemper and phocid distemper viruses. ppr has a very high rate of morbidity and mortality, and effective control of this disease is of economic importance in africa, asia and the mid ...19938212844
the detection of antibodies against peste des petits ruminants virus in cattle, sheep and goats and the possible implications to rinderpest control programmes.monoclonal antibody-based competitive elisa (c-elisa) have been used for the specific measurement of antibodies to both rinderpest and peste des petits ruminants (ppr) viruses in cattle, sheep and goats. examination of serum samples from sheep and goats in gambia, before and after vaccination with rinderpest vaccine, suggested that antibodies to ppr virus could prevent an immune response to the rinderpest vaccine. cattle sera from nigeria and ghana showed a high prevalence of antibody against pp ...19948119361
cloning and expression of the nucleoprotein of peste des petits ruminants virus in baculovirus for use in serological diagnosis.peste des petits ruminants (ppr) is a viral disease of goats and sheep characterized by erosive stomatitis, enteritis, and pneumonia. the virus is a member of the family paramyxoviridae and the genus morbillivirus. the disease has high morbidity and mortality rates and has a substantial economic impact in developing countries. we have cloned and sequenced the cdna of the nucleocapsid (n) gene of the nigeria 75/1 strain of ppr virus (pprv). a comparison of its nucleotide and deduced amino acid se ...19957747450
development of a competitive elisa for detecting antibodies to the peste des petits ruminants virus using a recombinant nucleoprotein.a competitive elisa based on the reaction between a monoclonal antibody (mab) and a recombinant nucleoprotein of the peste des petits ruminants virus (pprv) was developed. this protein was obtained in large quantities from insect cells infected with a ppr nucleoprotein recombinant baculovirus (n-b). the competitive elisa was compared with the virus neutralisation test (vnt) for detecting specific antibodies to pprv in sheep and goats. the time consuming vnt is the only prescribed test that is ca ...19957709061
differentiation of rinderpest and peste des petits ruminants viruses using specific cdna clones.the morbilliviruses which infect ruminants, rinderpest (rpv) and peste des petits ruminants (pprv), are difficult to distinguish serologically. they can be distinguished by differential neutralisation tests and by the migration of the major virus structural protein, the nucleocapsid protein, on polyacrylamide gels. both these methods are time consuming and require the isolation of live virus for identification; they are not suitable for analysis of material directly from post-mortem specimens. w ...19892723018
ppr virus infection on sheep in blacksea region of turkey: epidemiology and diagnosis by rt-pcr and virus isolation.in this study, a totally 164 materials (lung, spleen, lymph node, nasal and ocular swap, blood and samples from oral lesions) from sheep and lambs (n = 57) in the 34 flocks suspected the pprv infection as clinically and macroscopic pathologic remarks, housed in the 4 different provinces in the middle and eastern blacksea region were used for rt-pcr and virus isolation. additionally, serum samples randomly collected from 892 sheep were tested for the detection of pprv seroprevalance in the same r ...200918787968
reverse genetics for peste-des-petits-ruminants virus (pprv): promoter and protein specificities.peste-des-petits-ruminants virus (pprv) (family paramyxoviridae, genus morbillivirus) causes an acute febrile illness in sheep and goats resulting in significant morbidity and mortality in infected herds. the paramyxoviruses all have negative sense, non-segmented rna genomes and their host range and pathogenic determinants have been extensively studied using reverse genetics. this technology also enables a more rational approach to be taken with respect to vaccine design. in order to initiate th ...200717350130
an outbreak of peste des petits ruminants in a zoological collection.peste des petits ruminants virus was suspected to be the cause of a disease outbreak in a zoological collection at al ain in the arabian gulf. clinically the outbreak affected gazelles (gazellinae), ibex and sheep (caprinae) and gemsbok (hippotraginae); subclinical involvement of nilgai (tragelaphinae) was suspected. a morbillivirus was isolated and using monoclonal antibodies and biological tests in cattle, sheep and goats the virus of peste des petits ruminants was identified.19873424615
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