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safety evaluation of nuclear polyhedrosis virus replication in pigs.to evaluate the hygienic risk involved in using baculoviruses for insect pest control, safety studies are required. pigs were chosen as representative test animals of commercial and agricultural importance. the tests were aimed at detecting virus propagation, immune reactions, and signs of acute infection (changes in body temperature and hematology profile, swelling of lymph nodes). four of five animals inoculated with nuclear polyhedrosis virus showed a slight temperature rise at day 2 postinfe ...19836344789
uses of flow cytometry in virology.this article reviews some of the published applications of flow cytometry for in vitro and in vivo detection and enumeration of virus-infected cells. sample preparation, fixation, and permeabilization techniques for a number of virus-cell systems are evaluated. the use of flow cytometry for multiparameter analysis of virus-cell interactions for simian virus 40, herpes simplex viruses, human cytomegalovirus, and human immunodeficiency virus and its use for determining the effect of antiviral comp ...19947530594
construction and insect larval expression of recombinant vesicular stomatitis nucleocapsid protein and its use in competitive elisa.the gene encoding the nucleocapsid (n) protein of indiana 1 serotype vesicular stomatitis virus (vsv-in1) was transferred into the genome of autographa californica nuclear polyhedrosis virus (baculovirus) as a full-length non-fusion construct under the control of the polyhedrin gene promoter. recombinant n protein was obtained from trichoplusia ni insect larvae inoculated 72-96 h previously with the recombinant baculovirus. polyclonal antibody (pab) against vsv-in1 was produced in mice using vsv ...19958530566
detection of antibodies against porcine parvovirus nonstructural protein ns1 may distinguish between vaccinated and infected pigs.the humoral antibody response against the nonstructural protein ns1 and the structural protein vp2 of porcine parvovirus (ppv) was evaluated by immuno-peroxidase test (ipt) and enzyme linked immuno sorbent assay (elisa) using recombinant ppv antigens. the coding sequence for ns1 and vp2 was inserted into the baculovirus. autographa californica nuclear polyhedrosis virus (acnpv) genome resulting in two recombinant baculoviruses acnpv-ns1 and acnpv-vp2, respectively. sf9 cells (spodoptora frugidip ...19979050166
baculovirus treatment fully protects mice against a lethal challenge of fmdv.foot-and-mouth disease virus (fmdv) causes a highly contagious and economically devastating disease that affects cattle, swine, goat and sheep among others. fmdv is able to overcome the initial host innate immune response by inhibiting the induction of antiviral molecules at both the transcriptional and the translational levels. it has been demonstrated that fmdv a/arg/2001 causes the death of adult c57bl/6 mice within 72h. we evaluated the capacity of autographa californica nuclear polyhedrosis ...201020580746
large-scale production of porcine mature interleukin-18 (il-18) in silkworms using a hybrid baculovirus expression system.in this report, a hybrid baculovirus expression system, which means a hybrid virus of the autographa californica nuclear polyhedrosis virus and the bombyx mori nuclear polyhedrosis virus, was used for the large-scale production of porcine mature interleukin-18 (il-18) in silkworms. two recombinant hybrid baculoviruses containing cdna of the porcine precursor il-18 and the porcine caspase-1 were constructed and were used to infect silkworm larvae. after the co-infection of the two viruses, porcin ...200312655117
gene transfer into hepatocytes and human liver tissue by baculovirus vectors.gene therapy of liver diseases requires the development of efficient vectors for gene transfer in vivo. retroviral and adenoviral vectors have been shown to deliver genes efficiently into hepatocytes in vitro and in vivo. however, these vectors do not allow for exclusive infection of the liver which would be highly advantageous for in vivo gene therapy strategies. we have recently demonstrated that genetically modified baculoviruses (autographa californica nuclear polyhedrosis virus) efficiently ...19968930653
nucleocapsid protein n of lelystad virus: expression by recombinant baculovirus, immunological properties, and suitability for detection of serum antibodies.the orf7 gene, encoding the nucleocapsid protein n of lelystad virus (lv), was inserted downstream of the p10 promoter into autographa californica nuclear polyhedrosis virus (baculovirus). the resulting recombinant baculovirus, designated bac-orf7, expressed a 15-kda protein in insect cells. this protein was similar in size to the n protein expressed by lv in cl2621 cells when it was analyzed on sodium dodecyl sulfate-polyacrylamide gels. the n protein expressed by bac-orf7 was immunoprecipitate ...19958574824
cloning and expression of bovine and porcine interleukin-2 in baculovirus and analysis of species cross-reactivity.the cdnas encoding bovine and porcine interleukin-2 (il-2) have been expressed using the baculovirus autographa californica nuclear polyhedrosis virus as a vector in insect cells. insect cells infected with recombinant viruses secreted bovine and porcine il-2 into the culture medium, with biological activities for maintaining the proliferation of homologous cells. when the activities of these two il-2 proteins and commercially available human il-2 were tested on heterologous cells differences we ...19948042283
expression of the lassa virus nucleocapsid protein in insect cells infected with a recombinant baculovirus: application to diagnostic assays for lassa virus infection.the coding region of the gene for the nucleocapsid protein of lassa virus has been inserted into the genome of autographa californica nuclear polyhedrosis virus (acnpv) using the transfer vector pacym1, so that expression of the foreign dna is under the control of the promoter of the acnpv polyhedrin gene. infection of cultured spodoptera frugiperda cells with recombinant virus resulted in the synthesis of high levels of a protein that was indistinguishable from the authentic lassa virus protein ...19902406367
expression of htlv-i envelope protein fused to hydrophobic amino-terminal peptide of baculovirus polyhedrin in insect cells and its application for serological assays.the envelope of human t-cell leukemia virus type i (htlv-i) consists of two glycoproteins gp46 and p20e. recombinant envelope proteins were produced by using an expression vector derived from insect baculovirus, bombyx mori nuclear polyhedrosis virus. polyhedrin fusion proteins c182, n147, and n287 contained whole region p20e, c-terminal half of gp46, and almost whole region gp46, respectively. n147 and n287 were suggested to be processed forms resulting from internal cleavage by cellular enzyme ...19902078412
expression of biologically active recombinant porcine gm-csf by baculovirus gene expression system.the full length porcine granulocyte/macrophage colony stimulating factor (gm-csf) cdna, including secretion signal peptide coding region was recloned into baculovirus transfer vector pacym1. the vector was then transfected with autographica californica nuclear polyhedrosis virus (acnpv) dna into sf21ae cells and the recombinant virus acpgm was recovered. recombinant porcine gm-csf (rpgm-csf) was obtained from the serum-free culture medium of tn5 cells infected with the acpgm virus, and was shown ...19989682962
baculovirus as mammalian cell expression vector for gene therapy: an emerging strategy.the monopoly of insect cells to host baculovirus autographa californica multiple nuclear polyhedrosis virus (acmnpv) as a eukaryotic gene expression system has been shattered with the growing evidence that it also infects mammalian cells in culture. although acmnpv fails to replicate in vertebrate cells, it does express alien genes with levels of expression that are dependent on the strength of the promoter used to drive transcription of the foreign gene. it also has been reported that the recom ...200212095297
the use of a protein a conjugate in an indirect enzyme-linked immunosorbent assay (elisa) of four closely related baculoviruses from spodoptera species.the virus particles from four closely related insect nuclear polyhedrosis virus (npvs) isolated from their homologous spodoptera spp. hosts were examined using an indirect enzyme-linked immunosorbent assay (elisa), employing both swine anti-rabbit and protein a conjugate. results from both systems indicated that s. littoralis npv is serologically distinct from the other three viruses, s. frugiperda npv, s. exempta npv and s. exigua npv, which are closely related. the relatedness of these viruses ...19826754866
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