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molecular characterization of porcine picobirnaviruses and development of a specific reverse transcription-pcr assay.the molecular characterization of partial- length genomic segment 2 of porcine picobirnavirus (pbv) strains and the development of a specific reverse transcription-pcr (rt-pcr) assay for detection of virus in feces are reported. phylogenetic analysis indicated that the studied porcine isolates were more closely related (>85% identity) to human pbv belonging to genogroup i than to the other porcine pbv described so far. analysis by rt-pcr and polyacrylamide gel electrophoresis of fecal samples co ...200818508933
molecular characterization of picobirnaviruses from new hosts.picobirnaviruses (pbvs) have recently been classified into the picobirnaviridae family. they are small, non-enveloped viruses with bisegmented, double-stranded (ds) rna genomes. although they are found in the feces of a broad range of hosts, information regarding their genomes is limited to viruses detected from humans, rabbits, and porcine. identification of pbvs has been done using page and reverse transcription pcr (rt-pcr). in this study, we present a phylogenetic analysis of pbvs detected i ...200919463731
genogroup i picobirnaviruses in pigs: evidence for genetic diversity and relatedness to human strains.picobirnaviruses (pbvs) are small, non-enveloped viruses with a bisegmented double-stranded rna genome. their pathogenic potential, ecology, and evolutionary features are largely unexplored. here, we describe the molecular analysis of porcine pbvs identified in the intestinal content of dead pigs. six of 13 positive samples were cloned and then subjected to single-strand conformation polymorphism analysis and nucleotide sequencing. all clones belonged to genogroup i pbvs and almost all clones cl ...200818198385
serological and genomic characterization of porcine rotaviruses in thailand: detection of a g10 porcine rotavirus.a total of 557 fecal specimens collected from piglets with diarrhea in thailand were examined for rotavirus rna by polyacrylamide gel electrophoresis. twenty-three, one, and two samples were positive for group a, group b, and group c rotaviruses, respectively. two samples exhibited two segments found in picobirnavirus rna. rna electropherotyping of 23 group a rotaviruses showed that they were classified into five patterns. by serotyping by enzyme-linked immunosorbent assay and pcr, viruses in 3 ...19968727874
genogroup i and ii picobirnaviruses in respiratory tracts of pigs.sequence-independent amplification and specific reverse transcription pcrs identified genogroup i and ii picobirnaviruses in respiratory tracts of pigs. these data expand knowledge of picobirnavirus diversity and tropism. genetic relationships between porcine respiratory and human enteric picobirnaviruses suggest cross-species transmission of picobirnaviruses between pigs and humans.201122172405
picobirnavirus causes persistent infection in pigs.a study aimed to further understand the biology of porcine picobirnaviruses (pbv) was conducted between november 2003 and january 2008, on a farm located in the outskirts of córdoba city, argentina. pbv prevalence was examined by polyacrylamide gel electrophoresis and silver staining (page s/s) on a total of 265 samples collected from pigs divided into four groups, according to age and physiological status. pbv detection rate was highest in the group of sows sampled within the lactogenic period ...201020601172
porcine picobirnavirus in uk? 19902356603
evidence of closely related picobirnavirus strains circulating in humans and pigs in argentina.on the basis of the published literature, it is still difficult to draw conclusions as to whether picobirnavirus (pbv) circulation is influenced by host species restriction.201020888858
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