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detection of antibodies to mycoplasmas in south american camelids.indirect haemagglutination tests on sera from 757 south american camelids (alpacas, llamas and vicunas) carried out in the andean region of peru, revealed evidence of exposure mainly to mycoplasma mycoides subspecies mycoides lc. the incidence of detectable antibodies to this mycoplasma in 554 alpacas was 5.0 per cent and in 141 llamas 15.6 per cent. antibody to mycoplasma capricolum and the f38 biotype was detected in 0.9 per cent and 0.2 per cent of alpacas, respectively. in a group of 62 vicu ...19911780576
[in vitro antibiosensitivity of different strains of mycoplasma capricolum].the in vitro sensitivity of 20 wild strains of m. capricolum and that of the reference strain (california kid) against 14 antibiotics was investigated by means of a micromethod. the technique is based on the determination of the inhibitory minimum concentration (imc) in a liquid medium as revealed by the inhibition of glucose metabolism. the following results were obtained: all strains were sensitive to five antibiotics (tylosin, oxytetracyclin, gentamycin, neomycin, nalidixic acid) with an imc ...19902132784
taxonomy of the mycoplasma mycoides cluster.some of the mycoplasmas found in diseases of ruminants, mycoplasma capricolum, m. mycoides subsp. capri (pg3), bovine group 7 (pg50), strain f38 and strain y goat show various degrees of relationship to m. mycoides subsp. mycoides (pg1) and to one another, such that they are referred to as the m. mycoides cluster. studies using serology, dna homology, isoenzyme analysis and two-dimensional polyacrylamide gel electrophoresis (2-d page) of proteins have been carried out on the type and reference s ...19873312102
pathogenicity of mycoplasma capricolum in sheep after experimental infection.mycoplasma (m.) capricolum has been frequently isolated from diseased as well as from healthy sheep in morocco. in order to determine its pathogenicity for sheep, experiments were performed in three trials with the moroccan isolate 012. the following results were obtained: ewes inoculated intramammarily developed acute mastitis; the organism was transmitted to lambs suckling these ewes. only a mild mastitis appeared after a second inoculum, performed 5 weeks after the first. young lambs, 1 month ...19873660581
infection of sheep in great britain with mycoplasma capricolum. 19836665968
analysis of membrane proteins of mycoplasma capricolum strains by sds-page and immunoblotting.sixteen moroccan and five other mycoplasma (m.) capricolum strains were characterized by sds-page as well as by the immunobinding assay and immunoblotting, using antisera against m. capricolum california kid (ck) and m. mycoides yg. there was a strong reaction of all 21 strains with antiserum against m. capricolum ck and of 18 strains with antiserum against m. mycoides yg in the immunobinding assay, confirming the cross-reactivity between these two species observed earlier. a marked heterogeneit ...19938322544
dna relatedness between field isolates of mycoplasma f38 group, the agent of contagious caprine pleuropneumonia, and strains of mycoplasma capricolum.dna-dna hybridization experiments were carried out in order to clarify the taxonomic relationships between the f38 group of caprine mycoplasmas, the established etiologic agents of classical contagious caprine pleuropneumonia, and mycoplasma capricolum, an organism associated with septicemia, arthritis, and mastitis in goats and sheep. the taxonomic status of the f38 group has been uncertain, principally because of the serological, genomic, and other properties which it shares with m. capricolum ...19938347516
contagious caprine pleuropneumonia in uganda and isolation of mycoplasma capricolum subspecies capripneumoniae from goats and sheep. 19958748175
contagious caprine pleuropneumonia and other pulmonary mycoplasmoses of sheep and goats.contagious caprine pleuropneumonia (ccpp) is now a well-defined disease that is caused by mycoplasma capricolum subsp. capripneumoniae. ccpp is infectious, contagious and fulfils the classic koch postulates that characterise such types of disease. the distribution of the disease is not exactly known, but reports of mycoplasma isolation and official declarations to the office international des epizooties (oie) enable a probable distribution map to be obtained. there are many other mycoplasmas tha ...19969190020
polymerase chain reaction and restriction endonuclease digestion for selected members of the "mycoplasma mycoides cluster" and mycoplasma putrefaciens.a specific diagnostic method using the polymerase chain reaction, together with restriction endonuclease digestion patterns, was developed for members of the "mycoplasma mycoides cluster" that normally occur in the united states (i.e., mycoplasma mycoides subsp. mycoides large colony and mycoplasma capricolum subsp. capricolum in addition to "cluster" mycoplasma, bovine serogroup 7, and mycoplasma putrefaciens. the digestion of "cluster" polymerase chain reaction dna (1,225 bp) amplification pro ...19979211239
genetic and serological analysis of the immunogenic 67-kda lipoprotein of mycoplasma sp. bovine group 7.the gene encoding a lipoprotein of 67 kda, named p67, was cloned from mycoplasma sp. bovine group 7 strain pg50 and expressed in escherichia coli k12. analysis of the amino acid sequence derived from the dna sequence of the p67 gene revealed a typical prokaryotic signal peptidase ii membrane lipoprotein lipid attachment site and a transmembrane structure domain in the leader sequence at the amino-terminal end of the protein. protein p67 showed 91% identical amino acid residues to the lipoprotein ...19989766210
molecular analysis of field strains of mycoplasma capricolum subspecies capripneumoniae and mycoplasma mycoides subspecies mycoides, small colony type isolated from goats in tanzania.a molecular analysis of strains of mycoplasma capricolum subsp. capripneumoniae (m. capripneumoniae) and mycoplasma mycoides subsp. mycoides, small colony type (m. mycoides sc) isolated from goats was performed using the amplified fragment length polymorphism (aflp) and pulsed-field gel electrophoresis (pfge) fingerprinting techniques. among the 11 field strains of m. capripneumoniae from tanzanian goats, two aflp patterns were demonstrated, with 10 of the strains showing indistinguishable patte ...200111423192
contagious caprine pleuropneumonia and mannheimia haemolytica-associated acute respiratory disease of goats and sheep in afar region, ethiopia.in april 2002, an investigation into an outbreak of acute respiratory disease in goats and sheep in milae (afar), ethiopia was conducted. the investigation involved 4 flocks (722 sheep and 750 goats in total) and comprised the disease history, clinical and post-mortem examination, and microbiological analysis of nasal swabs, lung lesions, and pleural fluid samples. clinically diseased animals exhibited severe respiratory distress, and necropsy of two of the goats demonstrated fibrinous pneumonia ...200617361779
the in vitro effect of six antimicrobials against mycoplasma putrefaciens, mycoplasma mycoides subsp. mycoides lc and mycoplasma capricolum subsp. capricolum isolated from sheep and goats in jordan.respiratory disease in sheep and goats is a major problem in jordan and is often associated with mycoplasma species. without effective vaccines, control is mainly by chemotherapy, but the uncontrolled use of antimicrobials has led to concerns about the potential development of antimicrobial resistance. the in vitro effect of chloramphenicol, florfenicol, enrofloxacin, tylosin, erythromycin and oxytetracycline was determined against 32 isolates of mycoplasma species-m. mycoides subsp. mycoides lc ...200617405622
contagious caprine pleuropneumonia outbreak in captive wild ungulates at al wabra wildlife preservation, state of qatar.contagious caprine pleuropneumonia (ccpp) caused by mycoplasma capricolum subsp. capripneumoniae is a highly contagious and serious respiratory disease of domestic goats, characterized by coughing, severe respiratory distress, and high mortality rates. the lesions at necropsy are mainly a fibrinous pleuropneumonia with increased straw-colored pleural fluid. an outbreak of ccpp in wild goat (capra aegagrus), nubian ibex (capra ibex nubiana), laristan mouflon (ovis orientalis laristanica), and ger ...200717469281
in vitro antimycoplasmal activity of six jordanian medicinal plants against three mycoplasma species.the in vitro effect of six jordanian traditional medicine plant methanolic extracts were tested against 32 isolates of mycoplasma species; mycoplasma mycoides subsp. mycoides lc (6), mycoplasma capricolum subsp. capricolum (8) and m. putrefaciens (18), all isolated from either nasal swabs or milk, from sheep and goats in different regions in jordan. all mycoplasma species showed susceptibility to artemisia herba-alba and artemisia arborescens with mic ranges from 3.125-12.5 mg/ml. allium sativum ...200717969714
seroprevalence of contagious caprine pleuropneumonia in kefta humera, alamata (tigray) and aba-'ala (afar), northern ethiopia.a cross sectional study was conducted to determine the sero-prevalence of contagious caprine pleuroneumonia in three districts of tigray and afar regions of ethiopia namely; kefta humera, alamata and aba-'alla. proportions and chi-square test statistics were used to analyze the data. from a total of 863 goats and 137 sheep tested, 282 (32.68%) and 25 (18.25%) were positive for antibodies of mycoplasma capricolum subsp. capripneumoniae respectively using complement fixation test (cft). the seropr ...200918989743
detection of contagious caprine pleuropneumonia in east turkey.a study was implemented to investigate the presence of contagious caprine pleuropneumonia (ccpp) in east turkey. this study was based on clinical surveillance in the field, surveillance at regional slaughterhouses and regular submission of suspected lesions to regional laboratories. the results showed that the agent of ccpp, mycoplasma capricolum subspecies capripneumoniae (mccp), could be detected by culture and specific polymerase chain reaction from 37.5% (12/32) of lung samples taken from go ...200920462161
stimulation of tumor necrosis factor-alpha production by mycoplasmas and inhibition by dexamethasone in cultured astrocytes.elevated levels of tumor necrosis factor-alpha (tnf alpha) and other cytokines and eicosanoids in the central nervous system (cns) have been noted in several human neurologic diseases, including multiple sclerosis and aids dementia. recently it was shown that glial cells, especially astrocytes, are a major source of cytokines and eicosanoids. in the present study we have shown that astrocytes derived from fetal rat brain triggered by mycoplasmas produce tnf alpha and prostaglandin e2 (pge2). add ...19938495362
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