[decontamination of continual cell lines spontaneously infected with mycoplasmas].the continual cell lines of bovine kidneys mdbk and aubek, and porcine kidneys rpd and ibrs, spontaneously infected with mycoplasma arginini and acholeplasma laidlawii, were decontaminated by the method of selective elimination. two elimination procedures were modified to be used for the decontamination: one based on the reduction of infection by the light treatment of the cultures, the other based on the selection of mycoplasma-free cell population through cell clonation. on the basis of a long ...19863090766
structural relatedness of three ion-transport adenosine triphosphatases around their active sites of phosphorylation.three membrane-bound adenosine triphosphatases were investigated for homology in the sequence of four amino acids about the active site of phosphorylation. the atpases were as follows: sodium-potassium-dependent atpase from dog kidney, na,k-atpase; hydrogen-potassium-dependent atpase from hog gastric mucosa, h,k-atpase, an atpase similar to na,k-atpase; and an atpase activity in the plasma membrane of corn, zea mays, roots (cr-atpase), a higher plant atpase. a membrane preparation containing an ...19853156136
rapid detection of mycoplasma contamination in cell cultures using sybr green-based real-time polymerase chain reaction.we have developed a simple method for rapid detection of mycoplasma contamination in cell cultures using sybr green-based real-time polymerase chain reaction (pcr). to detect eight common contaminant mollicutes, including mycoplasma (m. arginini, m. fermentans, m. orale, m. hyorhinis, m. hominis, m. salivarium, m. pirum) and acholeplasma laidlawii, four primers were prepared based on the 23s rrna regions. using these primers and a minimum of 100 fg of mycoplasma genomic dna, the 23s rrna regions ...200616759150
comparative pcr analysis for detection of mycoplasma infections in continuous cell lines.mycoplasma contamination of cell lines is one of the major problems in cell culturing. about 15-35% of all cell lines are infected with a limited number of mycoplasma species of predominantly human, swine, or bovine origin. we examined the mycoplasma contamination status in 495 cell cultures by polymerase chain reaction (pcr) assay, microbiological culture method, and deoxyribonucleic acid-ribonucleic acid (dna-rna) hybridization, and in 103 cell cultures by pcr and dna-rna hybridization, in ord ...200211928999
tubular structures of mycoplasma gallisepticum and their possible participation in cell five strains of mycoplasma gallisepticum, a protein with a molecular mass of about 40 kda was detected by immunoblotting with anti-pig brain tubulin polyclonal and monoclonal antibodies. in eight other mycoplasma species similarly tested no reaction was observed. thin serial sections of m. gallisepticum and acholeplasma laidlawii cells examined by transmission electron microscopy revealed a submembrane system of tubules in m. gallisepticum but not in a. laidlawii. the intracellular spatial di ...19948012588
lipid-phase transition of phosphatidylglycerol in acholeplasma laidlawii membranes studied with phospholipase a2.phospholipase a2 was applied to follow the lipid phase transition of phosphatidylglycerol in the membrane of acholeplasma laidlawii. the conclusions were identical with those obtained with physicochemical techniques. in addition, it was shown that phosphatidylglycerol undergoes the lipid phase transition in the same temperature range as the bulk of the membrane lipid; phase segregation between various molecular species of phosphatidylglycerol occurs; exchange of this phospholipid between rigid a ...19826889758
identification of mycoplasma hyopneumoniae in formalin-fixed porcine lung, using an indirect immunoperoxidase indirect immunoperoxidase staining technique was evaluated for detection of mycoplasma hyopneumoniae in formalin-fixed, paraffin-embedded porcine lung. lungs from swine with induced (n = 4) or naturally occurring m hyopneumoniae infection (n = 31) were examined grossly, by light and immunofluorescent microscopy, and by an indirect immunoperoxidase test, using antibody raised in swine against m hyopneumoniae as the primary antibody. organisms stained by the indirect immunoperoxidase method wer ...19883056138
mystery swine disease in the netherlands: the isolation of lelystad early 1991, the dutch pig-industry was struck by the so-called mystery swine disease. large-scale laboratory investigations were undertaken to search for the etiological agent. we focused on isolating viruses and mycoplasmas, and we tested paired sera of affected sows for antibodies against ten known pig viruses. the mycoplasmas m. hyosynoviae, m. hyopneumoniae, and acholeplasma laidlawii, and the viruses encephalomyocarditis virus and porcine enterovirus types 2 and 7 were isolated from indi ...19911835211
[development of specific technics for the prevention of mycoplasma infections in swine].four experimental series were run in 2 experiments with 44 unweaned piglets to test non-inactivated vaccine from ts-mutant m-60 of mycoplasma (m.) arginini and from attenuated strains of ch-2 m. hyorhinis, ep-29 m. hyosynoviae, m. suipneumoniae, and b-1 acholeplasma laidlawii. similar deviations of clinical and immunological parameters were recorded from piglets inoculated with the above vaccine and infected with pathogenic mycoplasma cultures. these deviations, however, were less strongly prono ...19892619458
interspecies and intraspecies dna homology among established species of acholeplasma: a review.radiolabeled dna probes prepared in vitro by the nick translation method were used to determine the nucleotide sequence homology among the eight established and one unclassified species of acholeplasma. very little dna homology (2 to 21 percent) was found among these nine distinct species and the heteroduplexes showed at least 15 percent mismatching as determined by thermal elution endpoints. the data obtained by hybridization analyses paralleled the results obtained by the growth inhibition and ...19836433583
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