antibodies against certain bluetongue and epizootic haemorrhagic disease viral serotypes in indonesian ruminants.the orbiviruses contain several important viruses of livestock including bluetongue (bt) and epizootic haemorrhagic disease of deer (ehd) which share some group antigens. preliminary screening of sera for antibodies to orbiviruses by the agar gel immunodiffusion (agid) test has previously revealed widespread infections with the bt group in indonesia. however serum neutralization (sn) tests give a more accurate estimate of exposure to each serotype in the bt and ehd groups, and in this study were ...19911679575
coding strategy of the s rna segment of dugbe virus (nairovirus; bunyaviridae).the s rna segment of dugbe (dug) virus (nairovirus; bunyaviridae) was sequenced from three overlapping cdna clones and by primer extension. the s rna is 1712 nucleotides in length and contains one large open reading frame (orf) of 1326 nucleotides coding for a 49.4-kda protein on viral complementary (vc) rna. this protein in size corresponds to the dug nucleocapsid (n) protein (p. cash, 1985, j. gen. virol. 66, 141-148). the 49.4-kda product was expressed as a fusion protein with beta-galactosid ...19902327076
comparison of immunofluorescence and enzyme-linked immunosorbent assays for the serology of hantaan virus infections.three enzyme-linked immunosorbent assay (elisa) systems based upon different principles were developed for the serology of hantaan virus infections and compared with an indirect immunofluorescence assay (ifa). the indirect ifa was carried out with gamma-irradiated hantaan virus-infected and uninfected vero e6 cells fixed with ethanol (-70 degrees c) or acetone (20 degrees c) on drop slides and a fitc-coupled sheep anti-human ig preparation. atypical staining in the ifa was avoided by using ethan ...19892566619
simple procedure for preparation of bluetongue virus and epizootic hemorrhagic disease virus antigens for agar gel immunodiffusion.a simplified procedure was developed for preparing soluble antigen from two related orbiviruses, bluetongue and epizootic hemorrhagic disease viruses, for agar gel immunodiffusion. the antigens gave excellent results in both micro-agar gel diffusion (agar gel precipitin) and macro-agar gel diffusion (bluetongue immunodiffusion). minor modification in the spatial arrangement of reference antisera, commonly utilized in the agar gel immunodiffusion tests, was employed to reduce the possible develop ...19836140269
the public health laboratory service (phls) and its role in the control of zoonotic disease.the aetiology of zoonotic infections embraces organisms from every branch of the microbial world. in addition, zoonoses must be considered as a truly global problem, both in terms of their distribution and the measures required for their control. within the uk, zoonotic disease is considered to be less frequent than in some less developed parts of the world. however, its contribution to overall morbidity and mortality within the uk population is not well established. in an attempt to define the ...200010913770
[detection for anti-hantavirus igm in patient serum with silver enhanced dot immunogold filtration assay].to establish a more rapid and simple method for detecting anti-hantavirus igm in patient' s serum.200011498694
serological evidence for borna disease virus infection in humans, wild rodents and other vertebrates in finland.borna disease virus (bdv) can infect many vertebrate species, including humans. bdv infection may lead to meningoencephalomyelitis in animals. an association with human neuropsychiatric diseases has been reported, but the causal relationship between bdv and human disease remains unclear.200717129759
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