association of unique, isolated treponemes with bovine digital dermatitis lesions.this study used a pcr-based approach targeting 16s rrna gene fragments to determine the occurrence and association of the three bovine digital dermatitis (bdd) treponeme phylogroups within lesions found in cattle from the united kingdom. examination of 51 bdd lesions collected from infected cattle across the united kingdom revealed that bdd treponeme group 1 (treponema medium/treponema vincentii-like), group 2 (treponema phagedenis-like), and group 3 (treponema putidum/treponema denticola-like) ...200919144804
in vitro susceptibility of bovine digital dermatitis associated spirochaetes to antimicrobial agents.bovine digital dermatitis (bdd) is an infectious lameness in cattle, which has a large global impact in terms of animal welfare and cost. the majority of evidence suggests that spirochaetes are the aetiological agent of this disease. the aim of this study was to identify the susceptibility of bdd associated spirochaetes to a range of antimicrobial agents with a view to potential usage in vivo to treat this widespread cattle disease. a microdilution method was adapted to determine the in vitro su ...200919081208
three unique groups of spirochetes isolated from digital dermatitis lesions in uk cattle.bovine digital dermatitis (bdd) is a severe infectious cause of lameness which has spread through dairy cattle populations worldwide, causing serious welfare and agricultural problems. spirochetes are the main organisms implicated and have previously proven difficult to isolate. this study aimed to isolate and characterise the range of spirochetes associated with bdd in the uk. twenty-three spirochete isolates were obtained from 30 bdd lesions, which by 16s rrna gene and flab2 gene analysis clus ...200818243592
treponema putidum sp. nov., a medium-sized proteolytic spirochaete isolated from lesions of human periodontitis and acute necrotizing ulcerative far, little phenotypic heterogeneity has been detected in cultured oral treponemes with trypsin-like proteolytic activity, and all have been assigned to the species treponema denticola. however, comparisons of protein patterns and antigen expression in our collection of proteolytic oral treponemes occasionally identified isolates with a unique phenotype; e.g. strain omz 830 (=atcc 700768), which qualified as a 'pathogen-related oral spirochaete' due to the presence of a approximately 37 kda p ...200415280279
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